Sequence Alignment of the Sodium Potassium ATPase Protein (with Clustalw)a
Isoform Sequence Alignment
of the Sodium Potassium ATPase
alpha subunit
alpha1 MGKGVGRDKYEPAAVSEQGDKK--------GKKGKKDRDMDELKKEVSMDDHKLSLDELH 52
alpha2 MGRGAGREYSPAATTAENGG----------GKKKQKEKELDELKKEVAMDDHKLSLDELG 50
alpha3 MG-----DKKD-----DKDSPK--------KNKGKERRDLDDLKKEVAMTEHKMSVEEVC 42
alpha4 MGLWGKKGTVAPHDQSPRRRPKKGLIKKKMVKREKQKRNMEELKKEVVMDDHKLTLEELS 60
** . :: :: :::::***** * :**::::*:
alpha1 RKYGTDLSRGLTSARAAEILARDGPNALTPPPTTPEWIKFCRQLFGGFSMLLWIGAILCF 112
alpha2 RKYQVDLSKGLTNQRAQDVLARDGPNALTPPPTTPEWVKFCRQLFGGFSILLWIGAILCF 110
alpha3 RKYNTDCVQGLTHSKAQEILARDGPNALTPPPTTPEWVKFCRQLFGGFSILLWIGAILCF 102
alpha4 TKYSVDLTKGHSHQRAKEILTRGGPNTVTPPPTTPEWVKFCKQLFGGFSLLLWTGAILCF 120
** .* :* : :* ::*:*.***::*********:***:*******:*** ******
alpha1 LAYSIQAATEEEPQNDNLYLGVVLSAVVIITGCFSYYQEAKSSKIMESFKNMVPQQALVI 172
alpha2 LAYGIQAAMEDEPSNDNLYLGVVLAAVVIVTGCFSYYQEAKSSKIMDSFKNMVPQQALVI 170
alpha3 LAYGIQAGTEDDPSGDNLYLGIVLAAVVIITGCFSYYQEAKSSKIMESFKNMVPQQALVI 162
alpha4 VAYSIQIYFNEEPTKDNLYLSIVLSVVVIVTGCFSYYQEAKSSKIMESFKNMVPQQALVI 180
:**.** :::* *****.:**:.***:****************:*************
alpha1 RNGEKMSINAEEVVVGDLVEVKGGDRIPADLRIISANGCKVDNSSLTGESEPQTRSPDFT 232
alpha2 REGEKMQINAEEVVVGDLVEVKGGDRVPADLRIISSHGCKVDNSSLTGESEPQTRSPEFT 230
alpha3 REGEKMQVNAEEVVVGDLVEIKGGDRVPADLRIISAHGCKVDNSSLTGESEPQTRSPDCT 222
alpha4 RGGEKMQINVQEVVLGDLVEIKGGDRVPADLRLISAQGCKVDNSSLTGESEPQSRSPDFT 240
* ****.:*.:***:*****:*****:*****:**::****************:***: *
alpha1 NENPLETRNIAFFSTNCVEGTARGIVVYTGDRTVMGRIATLASGLEGGQTPIAAEIEHFI 292
alpha2 HENPLETRNICFFSTNCVEGTARGIVIATGDRTVMGRIATLASGLEVGRTPIAMEIEHFI 290
alpha3 HDNPLETRNITFFSTNCVEGTARGVVVATGDRTVMGRIATLASGLEVGKTPIAIEIEHFI 282
alpha4 HENPLETRNICFFSTNCVEGTARGIVIATGDSTVMGRIASLTSGLAVGQTPIAAEIEHFI 300
::******** *************:*: *** *******:*:*** *:**** ******
alpha1 HIITGVAVFLGVSFFILSLILEYTWLEAVIFLIGIIVANVPEGLLATVTVCLTLTAKRMA 352
alpha2 QLITGVAVFLGVSFFVLSLILGYSWLEAVIFLIGIIVANVPEGLLATVTVCLTLTAKRMA 350
alpha3 QLITGVAVFLGVSFFILSLILGYTWLEAVIFLIGIIVANVPEGLLATVTVCLTLTAKRMA 342
alpha4 HLITVVAVFLGVTFFALSLLLGYGWLEAIIFLIGIIVANVPEGLLATVTVCLTLTAKRMA 360
::** *******:** ***:* * ****:*******************************
alpha1 RKNCLVKNLEAVETLGSTSTICSDKTGTLTQNRMTVAHMWFDNQIHEADTTENQSGVSFD 412
alpha2 RKNCLVKNLEAVETLGSTSTICSDKTGTLTQNRMTVAHMWFDNQIHEADTTEDQSGATFD 410
alpha3 RKNCLVKNLEAVETLGSTSTICSDKTGTLTQNRMTVAHMWFDNQIHEADTTEDQSGTSFD 402
alpha4 RKNCLVKNLEAVETLGSTSTICSDKTGTLTQNRMTVAHMWFDMTVYEADTTEEQTGKTFT 420
****************************************** ::******:*:* :*
alpha1 KTSATWLALSRIAGLCNRAVFQANQENLPILKRAVAGDASESALLKCIELCCGSVKEMRE 472
alpha2 KRSPTWTALSRIAGLCNRAVFKAGQENISVSKRDTAGDASESALLKCIELSCGSVRKMRD 470
alpha3 KSSHTWVALSHIAGLCNRAVFKGGQDNIPVLKRDVAGDASESALLKCIELSSGSVKLMRE 462
alpha4 KSSDTWFMLARIAGLCNRADFKANQEILPIAKRATTGDASESALLKFIEQSYSSVAEMRE 480
* * ** *::******** *:..*: :.: ** .:********** ** . .** **:
alpha1 RYAKIVEIPFNSTNKYQLSIHKNPNTSEPQHLLVMKGAPERILDRCSSILLHGKEQPLDE 532
alpha2 RNPKVAEIPFNSTNKYQLSIHEREDSPQS-HVLVMKGAPERILDRCSTILVQGKEIPLDK 529
alpha3 RNKKVAEIPFNSTNKYQLSIHETEDPNDNRYLLVMKGAPERILDRCSTILLQGKEQPLDE 522
alpha4 KNPKVAEIPFNSTNKYQMSIHLREDSSQT-HVLMMKGAPERILEFCSTFLLNGQEYSMND 539
: *:.***********:*** :. : ::*:*********: **::*::*:* .::.
alpha1 ELKDAFQNAYLELGGLGERVLGFCHLFLPDEQFPEGFQFDTDDVNFPIDNLCFVGLISMI 592
alpha2 EMQDAFQNAYMELGGLGERVLGFCQLNLPSGKFPRGFKFDTDELNFPTEKLCFVGLMSMI 589
alpha3 EMKEAFQNAYLELGGLGERVLGFCHYYLPEEQFPKGFAFDCDDVNFTTDNLCFVGLMSMI 582
alpha4 EMKEAFQNAYLELGGLGERVLGFCFLNLPS-SFSKGFPFNTDEINFPMDNLCFVGLISMI 598
*:::******:************* **. .*..** *: *::**. ::******:***
alpha1 DPPRAAVPDAVGKCRSAGIKVIMVTGDHPITAKAIAKGVGIISEGNETVEDIAARLNIPV 652
alpha2 DPPRAAVPDAVGKCRSAGIKVIMVTGDHPITAKAIAKGVGIISEGNETVEDIAARLNIPM 649
alpha3 DPPRAAVPDAVGKCRSAGIKVIMVTGDHPITAKAIAKGVGIISEGNETVEDIAARLNIPV 642
alpha4 DPPRAAVPDAVSKCRSAGIKVIMVTGDHPITAKAIAKGVGIISEGTETAEEVAARLKIPI 658
***********.*********************************.**.*::****:**:
alpha1 SQVNPRDAKACVVHGSDLKDMTSEQLDDILKYHTEIVFARTSPQQKLIIVEGCQRQGAIV 712
alpha2 SQVNPREAKACVVHGSDLKDMTSEQLDEILKNHTEIVFARTSPQQKLIIVEGCQRQGAIV 709
alpha3 SQVNPRDAKACVIHGTDLKDFTSEQIDEILQNHTEIVFARTSPQQKLIIVEGCQRQGAIV 702
alpha4 SKVDASAAKAIVVHGAELKDIQSKQLDQILQNHPEIVFARTSPQQKLIIVEGCQRLGAVV 718
*:*:. *** *:**::***: *:*:*:**: *.********************* **:*
alpha1 AVTGDGVNDSPALKKADIGVAMGIAGSDVSKQAADMILLDDNFASIVTGVEEGRLIFDNL 772
alpha2 AVTGDGVNDSPALKKADIGIAMGISGSDVSKQAADMILLDDNFASIVTGVEEGRLIFDNL 769
alpha3 AVTGDGVNDSPALKKADIGVAMGIAGSDVSKQAADMILLDDNFASIVTGVEEGRLIFDNL 762
alpha4 AVTGDGVNDSPALKKADIGIAMGISGSDVSKQAADMILLDDNFASIVTGVEEGRLIFDNL 778
*******************:****:***********************************
alpha1 KKSIAYTLTSNIPEITPFLIFIIANIPLPLGTVTILCIDLGTDMVPAISLAYEQAESDIM 832
alpha2 KKSIAYTLTSNIPEITPFLLFIIANIPLPLGTVTILCIDLGTDMVPAISLAYEAAESDIM 829
alpha3 KKSIAYTLTSNIPEITPFLLFIMANIPLPLGTITILCIDLGTDMVPAISLAYEAAESDIM 822
alpha4 KKSIMYTLTSNIPEITPFLMFIILGIPLPLGTITILCIDLGTDMVPAISLAYESAESDIM 838
**** **************:**: .*******:******************** ******
alpha1 KRQPRNPKTDKLVNERLISMAYGQIGMIQALGGFFTYFVILAENGFLPIHLLGLRVDWDD 892
alpha2 KRQPRNSQTDKLVNERLISMAYGQIGMIQALGGFFTYFVILAENGFLPSRLLGIRLDWDD 889
alpha3 KRQPRNPRTDKLVNERLISMAYGQIGMIQALGGFFSYFVILAENGFLPGNLVGIRLNWDD 882
alpha4 KRLPRNPKTDNLVNHRLIGMAYGQIGMIQALAGFFTYFVILAENGFRPVDLLGIRLHWED 898
** ***.:**:***.***.************.***:********** * *:*:*:.*:*
alpha1 RWINDVEDSYGQQWTYEQRKIVEFTCHTAFFVSIVVVQWADLVICKTRRNSVFQQGMKNK 952
alpha2 RTMNDLEDSYGQEWTYEQRKVVEFTCHTAFFASIVVVQWADLIICKTRRNSVFQQGMKNK 949
alpha3 RTVNDLEDSYGQQWTYEQRKVVEFTCHTAFFVSIVVVQWADLIICKTRRNSVFQQGMKNK 942
alpha4 KYLNDLEDSYGQQWTYEQRKVVEFTCQTAFFVTIVVVQWADLIISKTRRNSLFQQGMRNK 958
: :**:******:*******:*****:****.:*********:*.******:*****:**
alpha1 ILIFGLFEETALAAFLSYCPGMGVALRMYPLKPTWWFCAFPYSLLIFVYDEVRKLIIRRR 1012
alpha2 ILIFGLLEETALAAFLSYCPGMGVALRMYPLKVTWWFCAFPYSLLIFIYDEVRKLILRRY 1009
alpha3 ILIFGLFEETALAAFLSYCPGMDVALRMYPLKPSWWFCAFPYSFLIFVYDEIRKLILRRN 1002
alpha4 VLIFGILEETLLAAFLSYTPGMDVALRMYPLKITWWLCAIPYSILIFVYDEIRKLLIRQH 1018
:****::*** ******* ***.********* :**:**:***:***:***:***::*:
alpha1 PGGWVEKETYY 1023
alpha2 PGGWVEKETYY 1020
alpha3 PGGWVEKETYY 1013
alpha4 PDGWVERETYY 1029
*.****:****
Accession numbers of the sodium potassium ATPase alpha subunits used in the protein sequence alignment: alpha1 - P05023; alpha2 - P50993; alpha3 - P13637; alpha4 - Q13733
beta subunit
beta1 -MARGKAKEEGS----WKKFIWNSEKKEFLGRTGGSWFKILLFYVIFYGCLAGIFIGTIQ 55
beta2 MVIQKEKKSCGQVVEEWKEFVWNPRTHQFMGRTGTSWAFILLFYLVFYGFLTAMFTLTMW 60
beta3 -MTKNEKKSLNQSLAEWKLFIYNPTTGEFLGRTAKSWGLILLFYLVFYGFLAALFSFTMW 59
: : : *. .. ** *::*. . :*:***. ** *****::*** *:.:* *:
beta1 VMLLTISEFKPTYQDRVAPPGLTQIPQ-IQKTEISFRPNDPKSYEAYVLNIVRFLEKYKD 114
beta2 VMLQTVSDHTPKYQDRLATPGLMIRPK-TENLDVIVNVSDTESWDQHVQKLNKFLEPYND 119
beta3 VMLQTLNDEVPKYRDQIPSPGLMVFPKPVTALEYTFSRSDPTSYAGYIEDLKKFLKPY-- 117
*** *:.: *.*:*::..*** *: : . .*. *: :: .: :**: *
beta1 SAQRDDMIFEDCGDVPSEPKERGDFNHERGERKVCRFKLEWLGNCSGLND-ETYGYKEGK 173
beta2 SIQAQKN-DVCRPGRYYEQPDNGVLNY---PKRACQFNRTQLGNCSGIGDSTHYGYSTGQ 175
beta3 TLEEQKNLTVCPDGALFEQKGP--------VYVACQFPISLLQACSGMND-PDFGYSQGN 168
: : :. . * .*:* * ***:.* :**. *:
beta1 PCIIIKLNRVLGFKPKPPKNESLETYPVMKYNPNVLPVQCTGKRDEDKDKVGNVEYFGLG 233
beta2 PCVFIKMNRVINFYAGA---------------NQSMNVTCAGKRDEDAENLGNFVMFPAN 220
beta3 PCILVKMNRIIGLKP-----------------EGVPRIDCVSKN----EDIPNVAVYPHN 207
**:::*:**::.: . : *..*. :.: *. : .
beta1 NSPGFPLQYYPYYGKLLQPKYLQPLLAVQFTNLTMD--TEIRIECKAY-GENIG-YSEKD 289
beta2 G--NIDLMYFPYYGKKFHVNYTQPLVAVKFLNVTPN--VEVNVECRIN-AANIATDDERD 275
beta3 G--MIDLKYFPYYGKKLHVGYLQPLVAVQVSFAPNNTGKEVTVECKIDGSANLKSQDDRD 265
. : * *:***** :: * ***:**:. . : *: :**: . *: .::*
beta1 RFQGRFDVKIEVKS- 303
beta2 KFAGRVAFKLRINKT 290
beta3 KFLGRVMFKITARA- 279
:* **. .*: .
Accession numbers of the sodium potassium ATPase beta subunits used in the protein sequence alignment: beta1 - P05026; beta2 - P14415 ; beta3 - P54709
Predicted General Properties of the Sodium Potassium ATPase Protein (Homo sapiens) - Primary Sequence Analysis (with ProtParam)b
alpha1
subunit sodium potassium ATPase
Number of amino acids: 1023
Molecular weight: 112896.1
Theoretical pI: 5.33
Total number of negatively charged residues (Asp + Glu): 125
Total number of positively charged residues (Arg + Lys): 102
Atomic composition: Carbon C 5047 Hydrogen H 8014 Nitrogen N 1338 Oxygen O 1495 Sulfur S 48
Formula: C5047H8014N1338O1495S48
Total number of atoms: 15942
Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 103135 Abs 0.1% (=1 g/l) 0.914, assuming ALL Cys residues appear as half cystines Ext. coefficient 101760 Abs 0.1% (=1 g/l) 0.901, assuming NO Cys residues appear as half cystines
Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo).
Instability index: The instability index (II) is computed to be 32.54 This classifies the protein as stable.
Aliphatic index: 97.42
Grand average of hydropathicity (GRAVY): 0.011
beta1
subunit sodium potassium atpase
Number of amino acids: 303
Molecular weight: 35061.3
Theoretical pI: 8.74
Total number of negatively charged residues (Asp + Glu): 40
Total number of positively charged residues (Arg + Lys): 46
Atomic composition: Carbon C 1598 Hydrogen H 2459 Nitrogen N 411 Oxygen O 453 Sulfur S 12
Formula: C1598H2459N411O453S12
Total number of atoms: 4933
Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 49195 Abs 0.1% (=1 g/l) 1.403, assuming ALL Cys residues appear as half cystines Ext. coefficient 48820 Abs 0.1% (=1 g/l) 1.392, assuming NO Cys residues appear as half cystines
Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo).
Instability index: The instability index (II) is computed to be 32.48 This classifies the protein as stable.
Aliphatic index: 73.30
Grand average of hydropathicity (GRAVY): -0.578
Bioinformatics References
a Higgins D., Thompson J., Gibson T. Thompson J. D., Higgins D. G., Gibson T. J.(1994). CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting,position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 22:4673-4680.
b Gasteiger E., Hoogland C., Gattiker A., Duvaud S., Wilkins M.R., Appel R.D., Bairoch A.; Protein Identification and Analysis Tools on the ExPASy Server;
(In) John M. Walker (ed): The Proteomics Protocols Handbook, Humana Press (2005). pp. 571-607 Full text - Copyright Humana Press.
