The Retinoblastoma (Rb1) Tumor Suppressor Protein
Sequence Alignment of the Retinoblastoma (Rb1) Tumor Suppressor Protein (with Clustalw)a
Species Sequence Alignment
of the Retinoblastoma Tumor Suppressor Rb1
Human MPPKTPRKTAATAAAAAAEPPAPPPPPPPEEDPEQDSGPEDLPLVRLEFEETEEPDFTAL 60
Mouse MPPKAPRR------AAAAEPPPPPPPPPREDDPAQDSGPEELPLARLEFEEIEEPEFIAL 54
Chicken MPPKPLRR------AGAARSQRTSP----EGGAGTASPPGGTRL------EVGEAEFVAL 44
****. *: *.**.. ..* * .. * * * * *.:* **
Human CQKLKIPDHVRERAWLTWEKVSSVDGVLGGYIQKKKELWGICIFIAAVDLDEMSFTFTEL 120
Mouse CQKLKVPDHVRERAWLTWEKVSSVDGILEGYIQKKKELWGICIFIAAVDLDEMPFTFTEL 114
Chicken CDALKAPDSVREKAWMTYQSLAAADGAS-AYNKKKKETWGVCIFIVAIDLDEMTFTFTEL 103
*: ** ** ***:**:*::.:::.** .* :**** **:****.*:*****.******
Human QKNIEISVHKFFNLLKEIDT-----STKVDNAMSRLLKKYDVLFALFSKLERTCELIYLT 175
Mouse QKSIETSVYKFFDLLKEIDT-----STKVDNAMSRLLKKYNVLCALYSKLERTCELIYLT 169
Chicken LKSLSISVCTFFQFLKEVDVNMDTVSTKVDSTVSRLKKKYDVLLALYHKFERTCGLIYLE 163
*.:. ** .**::***:*. *****.::*** ***:** **: *:**** ****
Human QPSSSISTEINSALVLKVSWITFLLAKGEVLQMEDDLVISFQLMLCVLDYFIKLSPPMLL 235
Mouse QPSSALSTEINSMLVLKISWITFLLAKGEVLQMEDDLVISFQLMLCVVDYFIKFSPPALL 229
Chicken QPSSEISAELSSVLVLKNYWITFLLAKGKVLQMEDDLVISFQLLLCVLDYFIKLSPPAML 223
**** :*:*:.* **** *********:**************:***:*****:*** :*
Human KEPYKTAV--IPINGSPRTPRRGQNRSARIAKQLENDTRIIEVLCKEHECNIDEVKNVYF 293
Mouse REPYKTAA--IPINGSPRTPRRGQNRSARIAKQLENDTRIIEVLCKEHECNIDEVKNVYF 287
Chicken KEPYKSAVTALTVNGSTRTPRRGQNRNARASKQIDTDTKVIEILCKEHDCNLDEVKNVYF 283
:****:*. :.:***.*********.** :**::.**::**:*****:**:********
Human KNFIPFMNSLGLVTSNGLPEVENLSKRYEEIYLKNKDLDARLFLDHDKTLQTDSIDSFET 353
Mouse KNFIPFINSLGIVSSNGLPEVESLSKRYEEVYLKNKDLDARLFLDHDKTLQTDPIDSFET 347
Chicken TSFIPFLNSLGVVASNGLPEVDVLSKQYDELYLKNKDIDARLFLDHDETLQPDVIACSQL 343
..****:****:*:*******: ***:*:*:******:*********:***.* * . :
Human QRTPRKSNLDEEVNVIPPHTPVRTVMNTIQQLMMILNSASDQPSENLISYFNNCTVNPKE 413
Mouse ERTPRKNNPDEEANVVTPHTPVRTVMNTIQQLMVILNSASDQPSENLISYFNNCTVNPKE 407
Chicken ERTPRKNNPDEEVNHVLPQTPVRAAMNTIQQLMMILNSATDKPSDTLIAYFNNCTVNPED 403
:*****.* ***.* : *:****:.********:*****:*:**:.**:*********::
Human SILKRVKDIGYIFKEKFAKAVGQGCVEIGSQRYKLGVRLYYRVMESMLKSEEERLSIQNF 473
Mouse NILKRVKDVGHIFKEKFANAVGQGCVDIGVQRYKLGVRLYYRVMESMLKSEEERLSIQNF 467
Chicken SILKRVESLGHIFKKKFAEAVGQGCAEIGSQRYQLGVRLYYRVMESMLKSEEERLSVHNF 463
.*****:.:*:***:***:******.:** ***:**********************::**
Human SKLLNDNIFHMSLLACALEVVMATYSRSTSQN--LDSGTDLSFPWILNVLNLKAFDFYKV 531
Mouse SKLLNDNIFHMSLLACALEVVMATYSRSTLQH--LDSGTDLSFPWILNVLNLKAFDFYKV 525
Chicken SKLLNDNIFHTSLLACALEIVMATYGRTASQSDGTSAETDLSFPWILNVFDLKAFDFYKV 523
********** ********:*****.*:: * .: ***********::*********
Human IESFIKAEGNLTREMIKHLERCEHRIMESLAWLSDSPLFDLIKQSKDREGPTDHLESACP 591
Mouse IESFIKVEANLTREMIKHLERCEHRIMESLAWLSDSPLFDLIKQSKDGEGP-DNLEPACP 584
Chicken IESFIKVEPSLTRDMIKHLERCEHRIMESLAWQSDSPLFDLIKQSKEREGQTDQPEPTST 583
******.* .***:****************** *************: ** *: *.:..
Human LNLPLQNNHTAADMYLSPVRSPKKKGSTTRVNSTANAETQATSAFQTQKPLKSTSLSLFY 651
Mouse LSLPLQGNHTAADMYLSPLRSPKKRTSTTRVNSAANTETQAASAFHTQKPLKSTSLALFY 644
Chicken LNLPLQHNHTAADLYLSPVRSPKKKASGHPQSGTSNPDAQPSATSQTQKPQKSTSLSLFY 643
*.**** ******:****:*****: * ..::*.::*.::: :**** *****:***
Human KKVYRLAYLRLNTLCERLLSEHPELEHIIWTLFQHTLQNEYELMRDRHLDQIMMCSMYGI 711
Mouse KKVYRLAYLRLNTLCARLLSDHPELEHIIWTLFQHTLQNEYELMRDRHLDQIMMCSMYGI 704
Chicken KKVFRLAYLRLHTLFFRLLSEHPDLEPLIWTLFQHTLQNESELMRDRHLDQIMMCSMYGI 703
***:*******:** ****:**:** :************ *******************
Human CKVKNIDLKFKIIVTAYKDLPHAVQETFKRVLIKEEEYDSIIVFYNSVFMQRLKTNILQY 771
Mouse CKVKNIDLKFKIIVTAYKDLPHAAQETFKRVLIREEEFDSIIVFYNSVFMQRLKTNILQY 764
Chicken CKVKNVDLRFKTIVSAYKELPNTNQETFKRVLIREEQYDSIIVFYNLVFMQKLKTNILQY 763
*****:**:** **:***:**:: *********:**::******** ****:********
Human ASTRPPTLSPIPHIPRSPYKFPSSPLRIPGGN-IYISPLKSPYKISEGLPTPTKMTPRSR 830
Mouse ASTRPPTLSPIPHIPRSPYKFSSSPLRIPGGN-IYISPLKSPYKISEGLPTPTKMTPRSR 823
Chicken ASNRPPTLSPIPHIPRSPYQFSNSPRRVPAGNNIYISPLKSPYKFSDGFHSPTKMTPRSR 823
**.****************:*..** *:*.** ***********:*:*: :*********
Human ILVSIGESFGTSEKFQKINQMVCNSDRVLKRSAEGSNPPKPLKKLRFDIEGSDEADGSKH 890
Mouse ILVSIGESFGTSEKFQKINQMVCNSDRVLKRSAEGGNPPKPLKNVRFDIEGADEADGSKH 883
Chicken ILVSIGETFGTSEKFQKINQMVCNSESHVKRSAEPSDAPKPLKRLRFDIEGQDEADGGKH 883
*******:*****************: :***** .:.*****.:****** *****.**
Human LPGESKFQQKLAEMTSTRTRMQKQKMNDSMDTSNKEEK 928
Mouse LPAESKFQQKLAEMTSTRTRMQKQRMNESKDVSNKEEK 921
Chicken LPQESKFQQKLAEMTSTRTRMQKQKLNDGNDTSANEEK 921
** *********************::*:. *.* :***
Accession Numbers of the Rb1 tumor suppressor protein sequences used in the protein sequence alignment: Human - NP_000312; Mouse - NP_033055; Chicken - NP_989750
Predicted General Properties of the Retinoblastoma (Rb1) Tumor Suppressor Protein (Homo sapiens) - Primary Sequence Analysis (with ProtParam)b
Number of amino acids: 928
Molecular weight: 106159.4
Theoretical pI: 8.13
Total number of negatively charged residues (Asp + Glu): 118
Total number of positively charged residues (Arg + Lys): 122
Atomic composition: Carbon C 4742 Hydrogen H 7563 Nitrogen N 1263 Oxygen O 1407 Sulfur S 43
Formula: C4742H7563N1263O1407S43
Total number of atoms: 15018
Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 81095 Abs 0.1% (=1 g/l) 0.764, assuming ALL Cys residues appear as half cystines Ext. coefficient 80220 Abs 0.1% (=1 g/l) 0.756, assuming NO Cys residues appear as half cystines
Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo).
Instability index: The instability index (II) is computed to be 47.85 This classifies the protein as unstable.
Aliphatic index: 86.78
Grand average of hydropathicity (GRAVY): -0.406
Bioinformatics References
a Higgins D., Thompson J., Gibson T. Thompson J. D., Higgins D. G., Gibson T. J.(1994). CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting,position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 22:4673-4680.
b Gasteiger E., Hoogland C., Gattiker A., Duvaud S., Wilkins M.R., Appel R.D., Bairoch A.; Protein Identification and Analysis Tools on the ExPASy Server;
(In) John M. Walker (ed): The Proteomics Protocols Handbook, Humana Press (2005). pp. 571-607 Full text - Copyright Humana Press.
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