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The Retinoblastoma (Rb1) Tumor Suppressor Protein

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Sequence Alignment of the Retinoblastoma (Rb1) Tumor Suppressor Protein (with Clustalw)a

Species Sequence Alignment of the Retinoblastoma Tumor Suppressor Rb1

Human       MPPKTPRKTAATAAAAAAEPPAPPPPPPPEEDPEQDSGPEDLPLVRLEFEETEEPDFTAL 60
Mouse       MPPKAPRR------AAAAEPPPPPPPPPREDDPAQDSGPEELPLARLEFEEIEEPEFIAL 54
Chicken     MPPKPLRR------AGAARSQRTSP----EGGAGTASPPGGTRL------EVGEAEFVAL 44
            ****. *:      *.**..  ..*    * ..   * *    *      *  *.:* **
Human       CQKLKIPDHVRERAWLTWEKVSSVDGVLGGYIQKKKELWGICIFIAAVDLDEMSFTFTEL 120
Mouse       CQKLKVPDHVRERAWLTWEKVSSVDGILEGYIQKKKELWGICIFIAAVDLDEMPFTFTEL 114
Chicken     CDALKAPDSVREKAWMTYQSLAAADGAS-AYNKKKKETWGVCIFIVAIDLDEMTFTFTEL 103
            *: ** ** ***:**:*::.:::.**   .* :**** **:****.*:*****.******
Human       QKNIEISVHKFFNLLKEIDT-----STKVDNAMSRLLKKYDVLFALFSKLERTCELIYLT 175
Mouse       QKSIETSVYKFFDLLKEIDT-----STKVDNAMSRLLKKYNVLCALYSKLERTCELIYLT 169
Chicken     LKSLSISVCTFFQFLKEVDVNMDTVSTKVDSTVSRLKKKYDVLLALYHKFERTCGLIYLE 163
             *.:. ** .**::***:*.     *****.::*** ***:** **: *:**** **** 
Human       QPSSSISTEINSALVLKVSWITFLLAKGEVLQMEDDLVISFQLMLCVLDYFIKLSPPMLL 235
Mouse       QPSSALSTEINSMLVLKISWITFLLAKGEVLQMEDDLVISFQLMLCVVDYFIKFSPPALL 229
Chicken     QPSSEISAELSSVLVLKNYWITFLLAKGKVLQMEDDLVISFQLLLCVLDYFIKLSPPAML 223
            **** :*:*:.* ****  *********:**************:***:*****:*** :*
Human       KEPYKTAV--IPINGSPRTPRRGQNRSARIAKQLENDTRIIEVLCKEHECNIDEVKNVYF 293
Mouse       REPYKTAA--IPINGSPRTPRRGQNRSARIAKQLENDTRIIEVLCKEHECNIDEVKNVYF 287
Chicken     KEPYKSAVTALTVNGSTRTPRRGQNRNARASKQIDTDTKVIEILCKEHDCNLDEVKNVYF 283
            :****:*.  :.:***.*********.** :**::.**::**:*****:**:********
Human       KNFIPFMNSLGLVTSNGLPEVENLSKRYEEIYLKNKDLDARLFLDHDKTLQTDSIDSFET 353
Mouse       KNFIPFINSLGIVSSNGLPEVESLSKRYEEVYLKNKDLDARLFLDHDKTLQTDPIDSFET 347
Chicken     TSFIPFLNSLGVVASNGLPEVDVLSKQYDELYLKNKDIDARLFLDHDETLQPDVIACSQL 343
            ..****:****:*:*******: ***:*:*:******:*********:***.* * . : 
Human       QRTPRKSNLDEEVNVIPPHTPVRTVMNTIQQLMMILNSASDQPSENLISYFNNCTVNPKE 413
Mouse       ERTPRKNNPDEEANVVTPHTPVRTVMNTIQQLMVILNSASDQPSENLISYFNNCTVNPKE 407
Chicken     ERTPRKNNPDEEVNHVLPQTPVRAAMNTIQQLMMILNSATDKPSDTLIAYFNNCTVNPED 403
            :*****.* ***.* : *:****:.********:*****:*:**:.**:*********::
Human       SILKRVKDIGYIFKEKFAKAVGQGCVEIGSQRYKLGVRLYYRVMESMLKSEEERLSIQNF 473
Mouse       NILKRVKDVGHIFKEKFANAVGQGCVDIGVQRYKLGVRLYYRVMESMLKSEEERLSIQNF 467
Chicken     SILKRVESLGHIFKKKFAEAVGQGCAEIGSQRYQLGVRLYYRVMESMLKSEEERLSVHNF 463
            .*****:.:*:***:***:******.:** ***:**********************::**
Human       SKLLNDNIFHMSLLACALEVVMATYSRSTSQN--LDSGTDLSFPWILNVLNLKAFDFYKV 531
Mouse       SKLLNDNIFHMSLLACALEVVMATYSRSTLQH--LDSGTDLSFPWILNVLNLKAFDFYKV 525
Chicken     SKLLNDNIFHTSLLACALEIVMATYGRTASQSDGTSAETDLSFPWILNVFDLKAFDFYKV 523
            ********** ********:*****.*:: *    .: ***********::*********
Human       IESFIKAEGNLTREMIKHLERCEHRIMESLAWLSDSPLFDLIKQSKDREGPTDHLESACP 591
Mouse       IESFIKVEANLTREMIKHLERCEHRIMESLAWLSDSPLFDLIKQSKDGEGP-DNLEPACP 584
Chicken     IESFIKVEPSLTRDMIKHLERCEHRIMESLAWQSDSPLFDLIKQSKEREGQTDQPEPTST 583
            ******.* .***:****************** *************: **  *: *.:..
          
     Human       LNLPLQNNHTAADMYLSPVRSPKKKGSTTRVNSTANAETQATSAFQTQKPLKSTSLSLFY 651
     Mouse       LSLPLQGNHTAADMYLSPLRSPKKRTSTTRVNSAANTETQAASAFHTQKPLKSTSLALFY 644
     Chicken     LNLPLQHNHTAADLYLSPVRSPKKKASGHPQSGTSNPDAQPSATSQTQKPQKSTSLSLFY 643
                 *.**** ******:****:*****: *    ..::*.::*.::: :**** *****:***
Human       KKVYRLAYLRLNTLCERLLSEHPELEHIIWTLFQHTLQNEYELMRDRHLDQIMMCSMYGI 711
Mouse       KKVYRLAYLRLNTLCARLLSDHPELEHIIWTLFQHTLQNEYELMRDRHLDQIMMCSMYGI 704
Chicken     KKVFRLAYLRLHTLFFRLLSEHPDLEPLIWTLFQHTLQNESELMRDRHLDQIMMCSMYGI 703
            ***:*******:**  ****:**:** :************ *******************
Human       CKVKNIDLKFKIIVTAYKDLPHAVQETFKRVLIKEEEYDSIIVFYNSVFMQRLKTNILQY 771
Mouse       CKVKNIDLKFKIIVTAYKDLPHAAQETFKRVLIREEEFDSIIVFYNSVFMQRLKTNILQY 764
Chicken     CKVKNVDLRFKTIVSAYKELPNTNQETFKRVLIREEQYDSIIVFYNLVFMQKLKTNILQY 763
            *****:**:** **:***:**:: *********:**::******** ****:********
Human       ASTRPPTLSPIPHIPRSPYKFPSSPLRIPGGN-IYISPLKSPYKISEGLPTPTKMTPRSR 830
Mouse       ASTRPPTLSPIPHIPRSPYKFSSSPLRIPGGN-IYISPLKSPYKISEGLPTPTKMTPRSR 823
Chicken     ASNRPPTLSPIPHIPRSPYQFSNSPRRVPAGNNIYISPLKSPYKFSDGFHSPTKMTPRSR 823
            **.****************:*..** *:*.** ***********:*:*: :*********
Human       ILVSIGESFGTSEKFQKINQMVCNSDRVLKRSAEGSNPPKPLKKLRFDIEGSDEADGSKH 890
Mouse       ILVSIGESFGTSEKFQKINQMVCNSDRVLKRSAEGGNPPKPLKNVRFDIEGADEADGSKH 883
Chicken     ILVSIGETFGTSEKFQKINQMVCNSESHVKRSAEPSDAPKPLKRLRFDIEGQDEADGGKH 883
            *******:*****************:  :***** .:.*****.:****** *****.**
Human       LPGESKFQQKLAEMTSTRTRMQKQKMNDSMDTSNKEEK 928
Mouse       LPAESKFQQKLAEMTSTRTRMQKQRMNESKDVSNKEEK 921
Chicken     LPQESKFQQKLAEMTSTRTRMQKQKLNDGNDTSANEEK 921
            ** *********************::*:. *.* :***
Accession Numbers of the Rb1 tumor suppressor protein sequences used in the protein sequence alignment: Human - NP_000312; Mouse - NP_033055; Chicken - NP_989750

Predicted General Properties of the Retinoblastoma (Rb1) Tumor Suppressor Protein  (Homo sapiens) - Primary Sequence Analysis (with ProtParam)b

Number of amino acids: 928

Molecular weight: 106159.4

Theoretical pI: 8.13

Total number of negatively charged residues (Asp + Glu): 118

Total number of positively charged residues (Arg + Lys): 122

Atomic composition: Carbon C 4742 Hydrogen H 7563 Nitrogen N 1263 Oxygen O 1407 Sulfur S 43

Formula: C4742H7563N1263O1407S43

Total number of atoms: 15018

Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 81095 Abs 0.1% (=1 g/l) 0.764, assuming ALL Cys residues appear as half cystines Ext. coefficient 80220 Abs 0.1% (=1 g/l) 0.756, assuming NO Cys residues appear as half cystines

Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo).

Instability index: The instability index (II) is computed to be 47.85 This classifies the protein as unstable.

Aliphatic index: 86.78

Grand average of hydropathicity (GRAVY): -0.406

Bioinformatics References

a Higgins D., Thompson J., Gibson T. Thompson J. D., Higgins D. G., Gibson T. J.(1994). CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting,position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 22:4673-4680.

b Gasteiger E., Hoogland C., Gattiker A., Duvaud S., Wilkins M.R., Appel R.D., Bairoch A.; Protein Identification and Analysis Tools on the ExPASy Server;
(In) John M. Walker (ed): The Proteomics Protocols Handbook, Humana Press (2005). pp. 571-607  Full text - Copyright Humana Press.

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