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The PTEN (Phosphatase and Tensin Homolog) Protein

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Sequence Alignment of the PTEN (Phosphatase and Tensin Homolog) Protein (with Clustalw)a

Species Sequence Alignment of PTEN

Human     MTAIIKEIVSRNKRRYQEDGFDLDLTYIYPNIIAMGFPAERLEGVYRNNIDDVVRFLDSK 60
Dog       MTAIIKEIVSRNKRRYQEDGFDLDLTYIYPNIIAMGFPAERLEGVYRNNIDDVVRFLDSK 60
Mouse     MTAIIKEIVSRNKRRYQEDGFDLDLTYIYPNIIAMGFPAERLEGVYRNNIDDVVRFLDSK 60
Rat       MTAIIKEIVSRNKRRYQEDGFDLDLTYIYPNIIAMGFPAERLEGVYRNNIDDVVRFLDSK 60
Zebrafish MTAKLKEIVSRNKRRYQEDGFDLDLTYIYPNIIAMGFPAERLEGVYRNNIDDVVRFLDSK 60
          *** :*******************************************************

Human     HKNHYKIYNLCAERHYDTAKFNCRVAQYPFEDHNPPQLELIKPFCEDLDQWLSEDDNHVA 120
Dog       HKNHYKIYNLCAERHYDTAKFNCRVAQYPFEDHNPPQLELIKPFCEDLDQWLSEDDNHVA 120
Mouse     HKNHYKIYNLCAERHYDTAKFNCRVAQYPFEDHNPPQLELIKPFCEDLDQWLSEDDNHVA 120
Rat       HKNHYKIYNLCAERHYDTAKFNCRVAQYPFEDHNPPQLELIKPFCEDLDQWLSEDDNHVA 120
Zebrafish HKNHYKIYNLCAERHYDASKFNCRVAQYPFEDHNPPQLELIKPFCEDLDQWLSEDENHVA 120
          *****************::************************************:****

Human     AIHCKAGKGRTGVMICAYLLHRGKFLKAQEALDFYGEVRTRDKKGVTIPSQRRYVYYYSY 180
Dog       AIHCKAGKGRTGVMICAYLLHRGKFLKAQEALDFYGEVRTRDKKGVTIPSQRRYVYYYSY 180
Mouse     AIHCKAGKGRTGVMICAYLLHRGKFLKAQEALDFYGEVRTRDKKGVTIPSQRRYVYYYSY 180
Rat       AIHCKAGKGRTGVMICAYLLHRGKFLKAQEALDFYGEVRTRDKKGVTIPSQRRYVYYYSY 180
Zebrafish AIHCKAGKGRTGVMICAYLLHRKKFAEAQEALDFYGEVRTRDKKGVTIPSQRRYVYYYSY 180
          ********************** ** :*********************************

Human     LLKNHLDYRPVALLFHKMMFETIPMFSGGTCN-----------------------PQFVV 217
Dog       LLKNHLDYRPVALLFHKMMFETIPMFSGGTCN-----------------------PQFVV 217
Mouse     LLKNHLDYRPVALLFHKMMFETIPMFSGGTCN-----------------------PQFVV 217
Rat       LLKNHLDYRPVALLFHKMMFETIPMFSGGTCN-----------------------PQFVV 217
Zebrafish LLKNKLDYKPVALLFHKMVFQTLPMFSGGTCRDSTVKNRSEVNPQGFKKGSWHWDPQFVV 240
          ****:***:*********:*:*:********.                       *****

Human     CQLKVKIYSSNSGPTRREDKFMYFEFPQPLPVCGDIKVEFFHKQNKMLKKDKMFHFWVNT 277
Dog       CQLKVKIYSSNSGPTRREDKFMYFEFPQPLPVCGDIKVEFFHKQNKMLKKDKMFHFWVNT 277
Mouse     CQLKVKIYSSNSGPTRREDKFMYFEFPQPLPVCGDIKVEFFHKQNKMLKKDKMFHFWVNT 277
Rat       CQLKVKIYSSNSGPTRREDKLMYFEFPQPLPVCGDIKVEFFHKQNKMLKKDKMFHFWVNT 277
Zebrafish YQLKVKIHTSNPAHTRREEKHMIFEFPQPLPVCGDIKVEFFHKQSKMMKKDKMFHFWINT 300
           ******::**.. ****:* * *********************.**:*********:**

Human     FFIPGPEETSEKVENGSLCDQEIDSICS---------------------------IERAD 310
Dog       FFIPGPEETSEKVENGSLCDQEIDSICS---------------------------IERAD 310
Mouse     FFIPGPEETSEKVENGSLCDQEIDSICS---------------------------IERAD 310
Rat       FFIPGPEETSEKVENGSLCDQEIDSICS---------------------------IERAD 310
Zebrafish FFIPGPEEAGDKVENGSVVN-DVDSLSCQSQSSPAERDREKERAVAAAALDRERGGERAD 359
          ********:.:******: : ::**:..                            ****

Human     NDKEYLVLTLTKNDLDKANKDKANRYFSPNFKVKLYFTKTVEEPSNPEASSSTSVTPDVS 370
Dog       NDKEYLVLTLTKNDLDKANKDKANRYFSPNFKVKLYFTKTVEEPSNPEASSSTSVTPDVS 370
Mouse     NDKEYLVLTLTKNDLDKANKDKANRYFSPNFKVKLYFTKTVEEPSNPEASSSTSVTPDVS 370
Rat       NDKEYLVLTLTKNDLDKANKDKANRYFSPNFKVKLYFTKTVEEPSNPEASSSTSVTPDVS 370
Zebrafish GEKDYLILTLTKNDLDKANKDKANRYFSPNFKVKLYFTKTVEEPSNSEASTSTSVTPDVS 419
          .:*:**:***************************************.***:*********

Human     DNEPDHYRYSDTTDSDPENEPFDEDQHTQITKV 403
Dog       DNEPDHYRYSDTTDSDPENEPFDEDQHTQITKV 403
Mouse     DNEPDHYRYSDTTDSDPENEPFDEDQHSQITKV 403
Rat       DNEPDHYRYSDTTDSDPENEPFDEDQHSQITKV 403
Zebrafish DNEPDHYRYSDTTDSDPENEPFDEEQHAQITKV 452
          ************************:**:*****

Accession numbers of the PTEN protein sequences used in the protein sequence alignment:  human - NP_000305; mouse - NP_032986; rat - NP_113794;
dog - NP_00100319; zebrafish - NP_957002

Predicted General Properties of the PTEN (Phosphatase and Tensin Homolog) Protein  (Homo sapiens) - Primary Sequence Analysis (with ProtParam)b

Number of amino acids: 403

Molecular weight: 47166.2

Theoretical pI: 5.94

Total number of negatively charged residues (Asp + Glu): 62

Total number of positively charged residues (Arg + Lys): 54

Atomic composition: Carbon C 2113 Hydrogen H 3215 Nitrogen N 561 Oxygen O 630 Sulfur S 19

Formula: C2113H3215N561O630S19

Total number of atoms: 6538

Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 45895 Abs 0.1% (=1 g/l) 0.973, assuming ALL Cys residues appear as half cystines Ext. coefficient 45270 Abs 0.1% (=1 g/l) 0.960, assuming NO Cys residues appear as half cystines

Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo).

Instability index: The instability index (II) is computed to be 44.21 This classifies the protein as unstable.

Aliphatic index: 66.97

Grand average of hydropathicity (GRAVY): -0.690

Bioinformatics References

a Higgins D., Thompson J., Gibson T. Thompson J. D., Higgins D. G., Gibson T. J.(1994). CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting,position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 22:4673-4680.

b Gasteiger E., Hoogland C., Gattiker A., Duvaud S., Wilkins M.R., Appel R.D., Bairoch A.; Protein Identification and Analysis Tools on the ExPASy Server;
(In) John M. Walker (ed): The Proteomics Protocols Handbook, Humana Press (2005). pp. 571-607  Full text - Copyright Humana Press.

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