Sequence Alignment of the Phenylalanine Hydroxylase Protein (with Clustalw)a
Species Sequence Alignment
of Phenylalanine Hydroxylase
mouse -MAAVVLENGVLSRKLSDFGQETSYIEDNSN-QNGAVSLIFSLKEEVGALAKVLRLFEEN 58
rat -MAAVVLENGVLSRKLSDFGQETSYIEDNSN-QNGAISLIFSLKEEVGALAKVLRLFEEN 58
human -MSTAVLENPGLGRKLSDFGQETSYIEDNCN-QNGAISLIFSLKEEVGALAKVLRLFEEN 58
chicken -------MDAQHCKMNGDSFQESTYTEEPSN-KNGVISLIFSLKEEVGALAKVLRTFEEK 52
fugu MRNMRAARPEVGLNVFVQKRIGSMYLEEEIGRKFEVISCIFSLKQEVGPLTKALKLFEEK 60
zebrafish ---MDAGLTQINGGLERKGSLTSSYLEEPLN-KSGVVSCIFSLKEEVGALVEALKLFEEK 56
. : * *: . : .:* *****:***.*.:.*: ***:
mouse EINLTHIESRPSRLNKDEYEFFTYLDKRSKPVLGSIIKSLRNDIGATVHELSRDKEKNTV 118
rat DINLTHIESRPSRLNKDEYEFFTYLDKRTKPVLGSIIKSLRNDIGATVHELSRDKEKNTV 118
human DVNLTHIESRPSRLKKDEYEFFTHLDKRSLPALTNIIKILRHDIGATVHELSRDKKKDTV 118
chicken GINLTHIESRPSRLNKDEYEFFINLEGKNVPALDKIIKSLRNDIGVTVHELSRTKKKDTV 112
fugu GINLTHIESRPSRTNTGQYEFFINVDTSCSQALDEVIDSLRTEISGHVHELSRNKEKDTV 120
zebrafish GINLTHIESRPSRKNNEEYEFFISVDQASSKVLDEIVDGLRTQIRGQVHELSRNKQKDTV 116
:*********** :. :**** :: .* .::. ** :* ****** *:*:**
mouse PWFPRTIQELDRFANQILSYGAELDADHPGFKDPVYRARRKQFADIAYNYRHGQPIPRVE 178
rat PWFPRTIQELDRFANQILSYGAELDADHPGFKDPVYRARRKQFADIAYNYRHGQPIPRVE 178
human PWFPRTIQELDRFANQILSYGAELDADHPGFKDPVYRARRKQFADIAYNYRHGQPIPRVE 178
chicken PWFPRSIQELDRFANQILSYGAELDADHPGFKDPVYRARRKEFADIAYNYRHGQPIPRVT 172
fugu PWFPNDIQDLDRFANQILSYGSELDSDHPGFTDQVYRLRRKEFADIAYNYRHGQPIPRVE 180
zebrafish PWFPNDIQDLDRFANQILSYGSELDADHPGFTDTVYRARRKEFADIAYNYRHGQPIPRVE 176
****. **:************:***:*****.* *** ***:*****************
mouse YTEEERKTWGTVFRTLKALYKTHACYEHNHIFPLLEKYCGFREDNIPQLEDVSQFLQTCT 238
rat YTEEEKQTWGTVFRTLKALYKTHACYEHNHIFPLLEKYCGFREDNIPQLEDVSQFLQTCT 238
human YMEEEKKTWGTVFKTLKSLYKTHACYEYNHIFPLLEKYCGFHEDNIPQLEDVSQFLQTCT 238
chicken YTEEEKKTWGTVFRELKNLYPTHACYEHNHVFPLLEKYCGYREDNIPQLEDVSKFLQTCT 232
fugu YTEDEKATWGKVFRELKTLYPTHACREHNRVFPLLEKYCGYKEDNIPQLEDVSRFLQSCT 240
zebrafish YTAEEKATWGTVFRELKTLYPTHACREHNRVFPLLEKYCGYREDNIPQLEDISHYLQSCT 236
* :*: ***.**: ** ** **** *:*::*********::*********:*::**:**
mouse GFRLRPVAGLLSSRDFLGGLAFRVFHCTQYIRHGSKPMYTPEPDICHELLGHVPLFSDRS 298
rat GFRLRPVAGLLSSRDFLGGLAFRVFHCTQYIRHGSKPMYTPEPDICHELLGHVPLFSDRS 298
human GFRLRPVAGLLSSRDFLGGLAFRVFHCTQYIRHGSKPMYTPEPDICHELLGHVPLFSDRS 298
chicken GFRLRPVAGLLSSRDFLAGLAFRVFHSTQYIRHASKPMYTPEPDICHELLGHVPLFADPS 292
fugu GFRLRPVAGLLSSRDFLAGLAFRVFHSTQYIRHSSRPTYTPEPDVCHELLGHVPLFADSS 300
zebrafish GFRLRPVAGLLSSRDFLAGLAFRVFHSTQYIRHSSKPMYTPEPDICHELLGHVPLFADPN 296
*****************.********.******.*:* ******:***********:* .
mouse FAQFSQEIGLASLGAPDEYIEKLATIYWFTVEFGLCKEGDSIKAYGAGLLSSFGELQYCL 358
rat FAQFSQEIGLASLGAPDEYIEKLATIYWFTVEFGLCKEGDSIKAYGAGLLSSFGELQYCL 358
human FAQFSQEIGLASLGAPDEYIEKLATIYWFTVEFGLCKQGDSIKAYGAGLLSSFGELQYCL 358
chicken FAQFSQEIGLASLGAPDDFIEKLATVYWFTVEFGLCKEGDSLKAYGAGLLSSFGELQYCL 352
fugu FAQFSQEIGLASLGAPDEYIEKLATVYWFTVEFGLCNQGSEIRAFGAGLLSSFGELQYCL 360
zebrafish FAQFSQEIGLASLGAPDEFIEKLATVYWFTVEFGLCKQGNEVKAYGAGLLSSFGELQYCL 356
*****************::******:**********::*..::*:***************
mouse SDKPKLLPLELEKTACQEYTVTEFRPLYYVAESFNDAKEKVRTFAATIPRPFSVRYDPYT 418
rat SDKPKLLPLELEKTACQEYSVTEFQPLYYVAESFSDAKEKVRTFAATIPRPFSVRYDPYT 418
human SEKPKLLPLELEKTAIQNYTVTEFQPLYYVAESFNDAKEKVRNFAATIPRPFSVRYDPYT 418
chicken SGKPEIRPLVLENTSVQKYSVTEFQPTYFVAESFNDAKEKLRKFAQTIPRPFSVRYNPYT 412
fugu TDKPKILPFDPDKTCLQKYPITEYQPVYFVADSFENAKEKVRKFAATIPKPFSVRYNPYT 420
zebrafish TNEPKLQPFEPEKTCQQKYPITEFQPVYFVAESFEDAKEKVRRFATTIPRPFSVRYNAYT 416
: :*:: *: ::*. *:*.:**::* *:**:**.:****:* ** ***:******:.**
mouse QRVEVLDNTQQLKNLADSINSEVGILCHALQKIKS 453
rat QRVEVLDNTQQLKILADSINSEVGILCNALQKIKS 453
human QRIEVLDNTQQLKILADSINSEIGILCSALQKIK- 452
chicken QRIEVLDNAKQLKNLADTINSEMGILCNALQKIK- 446
fugu QSVEVLDDTKQLTNLADCISSEMGKLCEALRKLQ- 454
zebrafish QRIEMLDNAQQLKNLADSINGEISILCNALRKM-- 449
* :*:**:::**. *** *..*:. ** **:*:
Accession numbers of the phenylalanine hydroxylase protein sequences used in the protein sequence alignment: Human - NP_000268; Mouse - NP_032803; Rat - NP_036751 ; Chicken -
NP_001001298; Fugu - NP_001027850; Zebrafish - NP_956845
Predicted General Properties of the Phenylalanine Hydroxylase Protein (Homo sapiens) - Primary Sequence Analysis (with ProtParam)b
Number of amino acids: 452
Molecular weight: 51862.0
Theoretical pI: 6.15
Total number of negatively charged residues (Asp + Glu): 60
Total number of positively charged residues (Arg + Lys): 54 Atomic composition: Carbon C 2350 Hydrogen H 3625 Nitrogen N 619 Oxygen O 683 Sulfur S 12
Formula: C2350H3625N619O683S12
Total number of atoms: 7289 Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 49780 Abs 0.1% (=1 g/l) 0.960, assuming ALL Cys residues appear as half cystines Ext. coefficient 49280 Abs 0.1% (=1 g/l) 0.950, assuming NO Cys residues appear as half cystines
Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo).
Instability index: The instability index (II) is computed to be 42.23 This classifies the protein as unstable.
Aliphatic index: 85.69
Grand average of hydropathicity (GRAVY): -0.376
Bioinformatics References
a Higgins D., Thompson J., Gibson T. Thompson J. D., Higgins D. G., Gibson T. J.(1994). CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting,position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 22:4673-4680.
b Gasteiger E., Hoogland C., Gattiker A., Duvaud S., Wilkins M.R., Appel R.D., Bairoch A.; Protein Identification and Analysis Tools on the ExPASy Server;
(In) John M. Walker (ed): The Proteomics Protocols Handbook, Humana Press (2005). pp. 571-607 Full text - Copyright Humana Press.
