Macromolecule Insights - Protein Bioinformatics Analysis

Privacy Policy

 


The Osteocalcin Protein

Bookmark and Share

Sequence Alignment of the Osteocalcin Protein (with Clustalw)a

Species and Isoform Sequence Alignment of Osteocalcin

Mouse1.1  MRTIFLLTLLTLAALCLSD---LTDAKPSGPESDK--AFMSKQEGNKVVNRLRRYL---- 51
Mouse1.2  MRTIFLLTLLTLAALCLSD---LTDAKPSGPESDK--AFMSKQEGNKVVNRLRRYL---- 51
Mouse2    MRTLSLLTLLALAALCLSD---LTDAKPSGPESDK--AFMSKQEGNKVVNRLRRYL---- 51
Rat       MRTLSLLTLLALTAFCLSD---LAGAKPSDSESDK--AFMSKQEGSKVVNRLRRYLNNGL 55
Cow       MRTPMLLALLALATLCLAG---RADAKPGDAESGKGAAFVSKQEGSEVVKRLRRYLDHWL 57
Sheep     MRTPML-ALLGLATLCLAG---QADAKPGDAESGKGTAFVSKQEGSEVVKRLRRYLDPGL 56
Human     MRALTLLALLALAALCIAG---QAGAKPSGAESSKGAAFVSKQEGSEVVKRPRRYLYQWL 57
Chicken   MKAAALLLLAALLTFSL----CRSAPDGSDARSAK--AFISHRQRAEMVRRQKRHYAQDS 54
Frog      MKLAIVLLLLGLAVLCLGGKDSQHSASAGDSRSSE--AFISRQDSANFARRHKRSYRYNV 58
          *:   :  *  * .:.:        .. ....* :  **:*:::  :...* :*      

Mouse1.1  GASVPSPDPLEPTREQCELNPACDELSDQYGLKTAYKRIYGITI 95
Mouse1.2  G------------------------------------------- 52
Mouse2    GASVPSPDPLEPTREQCELNPACDELSDQYGLKTAYKRIYGITI 95
Rat       GAPAPYPDPLEPHREVCELNPNCDELADHIGFQDAYKRIYGTTV 99
Cow       GAPAPYPDPLEPKREVCELNPDCDELADHIGFQEAYRRFYGPV- 100
Sheep     GAPAPYPDPLEPRREVCELNPDCDELADHIGFQEAYRRFYGPV- 99
Human     GAPVPYPDPLEPRREVCELNPDCDELADHIGFQEAYRRFYGPV- 100
Chicken   GVAGAPPNPLEAQREVCELSPDCDELADQIGFQEAYRRFYGPV- 97
Frog      ARGAAVTSPLESQREVCELNPDCDELADHIGFQEAYRRFYGPV- 101
          .                                           

Accession numbers of the osteocalcin protein sequences used in the protein sequence alignment:  NP_954642 osteocalcin [Human]; NP_031567 - bone gamma
carboxyglutamate protein 1 isoform 1 [Mouse1.1]; NP_001033028 bone gamma
carboxyglutamate protein 1 isoform 2 [Mouse1.2];NP_001027469 bone gamma-
carboxyglutamate protein 2 [Mouse2]; NP_038200 osteocalcin [Rat];
NP_776674 osteocalcin [Cow]; NP_001035098 bone gamma-carboxyglutamate
(gla) protein [Sheep]; NP_990718 osteocalcin [Chicken]; NP_001006689 bone
gamma-carboxyglutamate (gla) protein (osteocalcin) [Frog]

Predicted General Properties of the Osteocalcin Protein  (Homo sapiens) - Primary Sequence Analysis (with ProtParam)b

Number of amino acids: 100 

Molecular weight: 10962.5 

Theoretical pI: 6.56

Total number of negatively charged residues (Asp + Glu): 12

Total number of positively charged residues (Arg + Lys): 12

Atomic composition: Carbon C 491 Hydrogen H 770 Nitrogen N 136 Oxygen O 141 Sulfur S 4

ormula: C491H770N136O141S4

Total number of atoms: 1542

Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 13075 Abs 0.1% (=1 g/l) 1.193, assuming ALL Cys residues appear as half cystines Ext. coefficient 12950 Abs 0.1% (=1 g/l) 1.181, assuming NO Cys residues appear as half cystines

Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo).

Instability index: The instability index (II) is computed to be 54.38 This classifies the protein as unstable.

Aliphatic index: 86.00

Grand average of hydropathicity (GRAVY): -0.212

Bioinformatics References

a Higgins D., Thompson J., Gibson T. Thompson J. D., Higgins D. G., Gibson T. J.(1994). CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting,position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 22:4673-4680.

b Gasteiger E., Hoogland C., Gattiker A., Duvaud S., Wilkins M.R., Appel R.D., Bairoch A.; Protein Identification and Analysis Tools on the ExPASy Server;
(In) John M. Walker (ed): The Proteomics Protocols Handbook, Humana Press (2005). pp. 571-607  Full text - Copyright Humana Press.

Bookmark and Share