The Myoglobin Protein

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Sequence Alignment of the Myoglobin Protein (with Clustalw) a

Species Sequence Alignment 

Mouse        MGLSDGEWQLVLNVWGKVEADLAGHGQEVLIGLFKTHPETLDKFDKFKNLKSEEDMKGSE 60
Rat          MGLSDGEWQMVLNIWGKVEGDLAGHGQEVLISLFKAHPETLEKFDKFKNLKSEEEMKSSE 60
Human        MGLSDGEWQLVLNVWGKVEADIPGHGQEVLIRLFKGHPETLEKFDKFKHLKSEDEMKASE 60
Pig          MGLSDGEWQLVLNVWGKVEADVAGHGQEVLIRLFKGHPETLEKFDKFKHLKSEDEMKASE 60
Cow          MGLSDGEWQLVLNAWGKVEADVAGHGQEVLIRLFTGHPETLEKFDKFKHLKTEAEMKASE 60
Zebrafish    --MAD--HDLVLKCWGAVEADYAANGGEVLNRLFKEYPDTLKLFPKFSGIS-QGDLAGSP 55
               ::*   ::**: ** **.* ..:* ***  **. :*:**. * **. :. : :: .* 

Mouse        DLKKHGCTVLTALGTILKKKGQHAAEIQPLAQSHATKHKIPVKYLEFISEIIIEVLKKRH 120
Rat          DLKKHGCTVLTALGTILKKKGQHAAEIQPLAQSHATKHKIPVKYLEFISEVIIQVLKKRY 120
Human        DLKKHGATVLTALGGILKKKGHHEAEIKPLAQSHATKHKIPVKYLEFISECIIQVLQSKH 120
Pig          DLKKHGNTVLTALGGILKKKGHHEAELTPLAQSHATKHKIPVKYLEFISEAIIQVLQSKH 120
Cow          DLKKHGNTVLTALGGILKKKGHHEAEVKHLAESHANKHKIPVKYLEFISDAIIHVLHAKH 120
Zebrafish    AVAAHGATVLKKLGELLKAKGDHAALLKPLANTHANIHKVALNNFRLITEVLVKVMAEKA 115
              :  ** ***. ** :** **.* * :  **::**. **:.:: :.:*:: ::.*:  : 

Mouse        SGDFGADAQGAMSKALELFRNDIAAKYKELGFQG 154
Rat          SGDFGADAQGAMSKALELFRNDIAAKYKELGFQG 154
Human        PGDFGADAQGAMNKALELFRKDMASNYKELGFQG 154
Pig          PGDFGADAQGAMSKALELFRNDMAAKYKELGFQG 154
Cow          PSDFGADAQAAMSKALELFRNDMAAQYKVLGFHG 154
Zebrafish    G--LDAAGQGALRRVMDAVIGDIDGYYKEIGFAG 147
                :.* .*.*: :.:: .  *: . ** :** *

Accession Numbers: Human - NP_976312; Mouse - NP_038621; Rat - NP_067599; 
Cow - NP_776306; Pig - NP_999401; Zebrafish - NP_956880 

Predicted General Properties of the Myoglobin Protein  (Homo sapiens) - Primary Sequence Analysis (with ProtParam) b

Number of amino acids: 154 Molecular weight: 17183.8 Theoretical pI: 7.14
Amino acid composition: 
Ala (A) 12 7.8% Arg (R) 2 1.3% Asn (N) 3 1.9% Asp (D) 8 5.2% Cys (C) 1 0.6% Gln (Q) 7 4.5% Glu (E) 14 9.1% Gly (G) 15 9.7% His (H) 9 5.8% Ile (I) 8 5.2% Leu (L) 17 11.0% Lys (K) 20 13.0% Met (M) 4 2.6% Phe (F) 7 4.5% Pro (P) 5 3.2% Ser (S) 7 4.5% Thr (T) 4 2.6% Trp (W) 2 1.3% Tyr (Y) 2 1.3% Val (V) 7 4.5% Asx (B) 0 0.0% Glx (Z) 0 0.0% Xaa (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 22 Total number of positively charged residues (Arg + Lys): 22 Atomic composition: Carbon C 774 Hydrogen H 1224 Nitrogen N 210 Oxygen O 222 Sulfur S 5 Formula: C774H1224N210O222S5 Total number of atoms: 2435 Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 13980 Abs 0.1% (=1 g/l) 0.814, assuming ALL Cys residues appear as half cystines Ext. coefficient 13980 Abs 0.1% (=1 g/l) 0.814, assuming NO Cys residues appear as half cystines Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 21.81 This classifies the protein as stable. Aliphatic index: 84.29 Grand average of hydropathicity (GRAVY): -0.476

Bioinformatics References

a Higgins D., Thompson J., Gibson T. Thompson J. D., Higgins D. G., Gibson T. J.(1994). CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting,position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 22:4673-4680.

b Gasteiger E., Hoogland C., Gattiker A., Duvaud S., Wilkins M.R., Appel R.D., Bairoch A.; Protein Identification and Analysis Tools on the ExPASy Server;
(In) John M. Walker (ed): The Proteomics Protocols Handbook, Humana Press (2005). pp. 571-607  Full text - Copyright Humana Press.

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Recent Articles on the Myoglobin Protein

pubmed: myoglobin
Sertraline-Induced Rhabdomyolysis in an Elderly Patient with Dementia and Comorbidities (July/August).
Gareri P, Segura-García C, De Fazio P, De Fazio S, De Sarro G
Ann Pharmacother 


 

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