The Myoglobin Protein
Sequence Alignment of the Myoglobin Protein (with Clustalw)a
Species Sequence Alignment
of Myoglobin
Mouse MGLSDGEWQLVLNVWGKVEADLAGHGQEVLIGLFKTHPETLDKFDKFKNLKSEEDMKGSE 60
Rat MGLSDGEWQMVLNIWGKVEGDLAGHGQEVLISLFKAHPETLEKFDKFKNLKSEEEMKSSE 60
Human MGLSDGEWQLVLNVWGKVEADIPGHGQEVLIRLFKGHPETLEKFDKFKHLKSEDEMKASE 60
Pig MGLSDGEWQLVLNVWGKVEADVAGHGQEVLIRLFKGHPETLEKFDKFKHLKSEDEMKASE 60
Cow MGLSDGEWQLVLNAWGKVEADVAGHGQEVLIRLFTGHPETLEKFDKFKHLKTEAEMKASE 60
Zebrafish --MAD--HDLVLKCWGAVEADYAANGGEVLNRLFKEYPDTLKLFPKFSGIS-QGDLAGSP 55
::* ::**: ** **.* ..:* *** **. :*:**. * **. :. : :: .*
Mouse DLKKHGCTVLTALGTILKKKGQHAAEIQPLAQSHATKHKIPVKYLEFISEIIIEVLKKRH 120
Rat DLKKHGCTVLTALGTILKKKGQHAAEIQPLAQSHATKHKIPVKYLEFISEVIIQVLKKRY 120
Human DLKKHGATVLTALGGILKKKGHHEAEIKPLAQSHATKHKIPVKYLEFISECIIQVLQSKH 120
Pig DLKKHGNTVLTALGGILKKKGHHEAELTPLAQSHATKHKIPVKYLEFISEAIIQVLQSKH 120
Cow DLKKHGNTVLTALGGILKKKGHHEAEVKHLAESHANKHKIPVKYLEFISDAIIHVLHAKH 120
Zebrafish AVAAHGATVLKKLGELLKAKGDHAALLKPLANTHANIHKVALNNFRLITEVLVKVMAEKA 115
: ** ***. ** :** **.* * : **::**. **:.:: :.:*:: ::.*: :
Mouse SGDFGADAQGAMSKALELFRNDIAAKYKELGFQG 154
Rat SGDFGADAQGAMSKALELFRNDIAAKYKELGFQG 154
Human PGDFGADAQGAMNKALELFRKDMASNYKELGFQG 154
Pig PGDFGADAQGAMSKALELFRNDMAAKYKELGFQG 154
Cow PSDFGADAQAAMSKALELFRNDMAAQYKVLGFHG 154
Zebrafish G--LDAAGQGALRRVMDAVIGDIDGYYKEIGFAG 147
:.* .*.*: :.:: . *: . ** :** *
Accession Numbers of the myoglobin protein sequences used in the protein sequence alignment: Human - NP_976312; Mouse - NP_038621; Rat - NP_067599;
Cow - NP_776306; Pig - NP_999401; Zebrafish - NP_956880
Predicted General Properties of the Myoglobin Protein (Homo sapiens) - Primary Sequence Analysis (with ProtParam)b
Number of amino acids: 154
Molecular weight: 17183.8
Theoretical pI: 7.14
Total number of negatively charged residues (Asp + Glu): 22
Total number of positively charged residues (Arg + Lys): 22
Atomic composition: Carbon C 774 Hydrogen H 1224 Nitrogen N 210 Oxygen O 222 Sulfur S 5
Formula: C774H1224N210O222S5
Total number of atoms: 2435
Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 13980 Abs 0.1% (=1 g/l) 0.814, assuming ALL Cys residues appear as half cystines Ext. coefficient 13980 Abs 0.1% (=1 g/l) 0.814, assuming NO Cys residues appear as half cystines
Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo).
Instability index: The instability index (II) is computed to be 21.81 This classifies the protein as stable.
Aliphatic index: 84.29
Grand average of hydropathicity (GRAVY): -0.476
Bioinformatics References
a Higgins D., Thompson J., Gibson T. Thompson J. D., Higgins D. G., Gibson T. J.(1994). CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting,position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 22:4673-4680.
b Gasteiger E., Hoogland C., Gattiker A., Duvaud S., Wilkins M.R., Appel R.D., Bairoch A.; Protein Identification and Analysis Tools on the ExPASy Server;
(In) John M. Walker (ed): The Proteomics Protocols Handbook, Humana Press (2005). pp. 571-607 Full text - Copyright Humana Press.
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