Macromolecule Insights - Protein Bioinformatics Analysis

Privacy Policy

 


The Myoglobin Protein

Bookmark and Share

Sequence Alignment of the Myoglobin Protein (with Clustalw)a

Species Sequence Alignment of Myoglobin

Mouse        MGLSDGEWQLVLNVWGKVEADLAGHGQEVLIGLFKTHPETLDKFDKFKNLKSEEDMKGSE 60
Rat          MGLSDGEWQMVLNIWGKVEGDLAGHGQEVLISLFKAHPETLEKFDKFKNLKSEEEMKSSE 60
Human        MGLSDGEWQLVLNVWGKVEADIPGHGQEVLIRLFKGHPETLEKFDKFKHLKSEDEMKASE 60
Pig          MGLSDGEWQLVLNVWGKVEADVAGHGQEVLIRLFKGHPETLEKFDKFKHLKSEDEMKASE 60
Cow          MGLSDGEWQLVLNAWGKVEADVAGHGQEVLIRLFTGHPETLEKFDKFKHLKTEAEMKASE 60
Zebrafish    --MAD--HDLVLKCWGAVEADYAANGGEVLNRLFKEYPDTLKLFPKFSGIS-QGDLAGSP 55
               ::*   ::**: ** **.* ..:* ***  **. :*:**. * **. :. : :: .* 
Mouse        DLKKHGCTVLTALGTILKKKGQHAAEIQPLAQSHATKHKIPVKYLEFISEIIIEVLKKRH 120
Rat          DLKKHGCTVLTALGTILKKKGQHAAEIQPLAQSHATKHKIPVKYLEFISEVIIQVLKKRY 120
Human        DLKKHGATVLTALGGILKKKGHHEAEIKPLAQSHATKHKIPVKYLEFISECIIQVLQSKH 120
Pig          DLKKHGNTVLTALGGILKKKGHHEAELTPLAQSHATKHKIPVKYLEFISEAIIQVLQSKH 120
Cow          DLKKHGNTVLTALGGILKKKGHHEAEVKHLAESHANKHKIPVKYLEFISDAIIHVLHAKH 120
Zebrafish    AVAAHGATVLKKLGELLKAKGDHAALLKPLANTHANIHKVALNNFRLITEVLVKVMAEKA 115
              :  ** ***. ** :** **.* * :  **::**. **:.:: :.:*:: ::.*:  : 
Mouse        SGDFGADAQGAMSKALELFRNDIAAKYKELGFQG 154
Rat          SGDFGADAQGAMSKALELFRNDIAAKYKELGFQG 154
Human        PGDFGADAQGAMNKALELFRKDMASNYKELGFQG 154
Pig          PGDFGADAQGAMSKALELFRNDMAAKYKELGFQG 154
Cow          PSDFGADAQAAMSKALELFRNDMAAQYKVLGFHG 154
Zebrafish    G--LDAAGQGALRRVMDAVIGDIDGYYKEIGFAG 147
                :.* .*.*: :.:: .  *: . ** :** *
Accession Numbers of the myoglobin protein sequences used in the protein sequence alignment: Human - NP_976312; Mouse - NP_038621; Rat - NP_067599; Cow - NP_776306; Pig - NP_999401; Zebrafish - NP_956880

Predicted General Properties of the Myoglobin Protein  (Homo sapiens) - Primary Sequence Analysis (with ProtParam)b

Number of amino acids: 154

Molecular weight: 17183.8

Theoretical pI: 7.14

Total number of negatively charged residues (Asp + Glu): 22

Total number of positively charged residues (Arg + Lys): 22

Atomic composition: Carbon C 774 Hydrogen H 1224 Nitrogen N 210 Oxygen O 222 Sulfur S 5

Formula: C774H1224N210O222S5

Total number of atoms: 2435

Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 13980 Abs 0.1% (=1 g/l) 0.814, assuming ALL Cys residues appear as half cystines Ext. coefficient 13980 Abs 0.1% (=1 g/l) 0.814, assuming NO Cys residues appear as half cystines

Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo).

Instability index: The instability index (II) is computed to be 21.81 This classifies the protein as stable.

Aliphatic index: 84.29

Grand average of hydropathicity (GRAVY): -0.476

Bioinformatics References

a Higgins D., Thompson J., Gibson T. Thompson J. D., Higgins D. G., Gibson T. J.(1994). CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting,position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 22:4673-4680.

b Gasteiger E., Hoogland C., Gattiker A., Duvaud S., Wilkins M.R., Appel R.D., Bairoch A.; Protein Identification and Analysis Tools on the ExPASy Server;
(In) John M. Walker (ed): The Proteomics Protocols Handbook, Humana Press (2005). pp. 571-607  Full text - Copyright Humana Press.

Bookmark and Share