Sequence Alignment of the Muscarinic Acetylcholine Receptors (Homo sapiens) (with Clustalw)a
Isoform Sequence Alignment
of Muscarinic Acetylcholine Receptors
M1 -------------MNTSAPPAVSPNITVLAPGKG-------------------------- 21
M5 -------MEGDSYHNATTVNGTPVNHQPLERHR--------------------------- 26
M3 MTLHNNSTTSPLFPNISSSWIHSPSDAGLPPGTVTHFGSYNVSRAAGNFSSPDGTTDDPL 60
M2 ------------MNNST----NSSNNSLALTSP--------------------------- 17
M4 ------------MANFTPVNGSSGNQSVRLVTSSSH------------------------ 24
* : . .
M1 ----PWQVAFIGITTGLLSLATVTGNLLVLISFKVNTELKTVNNYFLLSLACADLIIGTF 77
M5 ----LWEVITIAAVTAVVSLITIVGNVLVMISFKVNSQLKTVNNYYLLSLACADLIIGIF 82
M3 GGHTVWQVVFIAFLTGILALVTIIGNILVIVSFKVNKQLKTVNNYFLLSLACADLIIGVI 120
M2 --YKTFEVVFIVLVAGSLSLVTIIGNILVMVSIKVNRHLQTVNNYFLFSLACADLIIGVF 75
M4 NRYETVEMVFIATVTGSLSLVTVVGNILVMLSIKVNRQLQTVNNYFLFSLACADLIIGAF 84
:: * :. ::* *: **:**::*:*** .*:*****:*:********** :
M1 SMNLYTTYLLMGHWALGTLACDLWLALDYVASNASVMNLLLISFDRYFSVTRPLSYRAKR 137
M5 SMNLYTTYILMGRWALGSLACDLWLALDYVASNASVMNLLVISFDRYFSITRPLTYRAKR 142
M3 SMNLFTTYIIMNRWALGNLACDLWLAIDYVASNASVMNLLVISFDRYFSITRPLTYRAKR 180
M2 SMNLYTLYTVIGYWPLGPVVCDLWLALDYVVSNASVMNLLIISFDRYFCVTKPLTYPVKR 135
M4 SMNLYTVYIIKGYWPLGAVVCDLWLALDYVVSNASVMNLLIISFDRYFCVTKPLTYPARR 144
****:* * : . *.** :.******:***.*********:*******.:*:**:* .:*
M1 TPRRAALMIGLAWLVSFVLWAPAILFWQYLVGERTVLAGQCYIQFLSQPIITFGTAMAAF 197
M5 TPKRAGIMIGLAWLISFILWAPAILCWQYLVGKRTVPLDECQIQFLSEPTITFGTAIAAF 202
M3 TTKRAGVMIGLAWVISFVLWAPAILFWQYFVGKRTVPPGECFIQFLSEPTITFGTAIAAF 240
M2 TTKMAGMMIAAAWVLSFILWAPAILFWQFIVGVRTVEDGECYIQFFSNAAVTFGTAIAAF 195
M4 TTKMAGLMIAAAWVLSFVLWAPAILFWQFVVGKRTVPDNQCFIQFLSNPAVTFGTAIAAF 204
*.: *.:**. **::**:******* **:.** *** .:* ***:*:. :*****:***
M1 YLPVTVMCTLYWRIYRETENRARELAALQGSETPGKG----------------------- 234
M5 YIPVSVMTILYCRIYRETEKRTKDLADLQGSDSVTKAEKRKPAHRALFRSCLRCPRPTLA 262
M3 YMPVTIMTILYWRIYKETEKRTKELAGLQASGTEAETENFVHPTGSS-RSCSSYELQQQS 299
M2 YLPVIIMTVLYWHISRASKSRIKKDKKEPVANQDPVSPSLVQ------------------ 237
M4 YLPVVIMTVLYIHISLASRSRVHKHRPEGPKEKKAKTLAFLK------------------ 246
*:** :* ** :* :..* :.
M1 ----GGSSSS------SERSQPGAEGSPETPPGRCCRCCRAPRLLQAYSWKEEEEED--- 281
M5 QRERNQASWS------SSRRSTSTTGKPSQATGPSANWAKAEQLTTCSSYPSSEDEDKPA 316
M3 MKRSNRRKYGRCHFWFTTKSWKPSSEQMDQDHSSSDSWNNNDAAASLENSASSDEEDIGS 359
M2 ----GRIVKP------NNNNMP---SSDDGLEHNKIQNGKAPRDPVTENCVQGEEKESSN 284
M4 ----SPLMKQ------SVKKPPPGEAAREELRNGKLEEAPPPALPPPPRPVA--DKDTSN 294
. . . . :::
M1 -EGSMESLTS---------SEGEE-PG---SEVVIKMPMVDPEAQAPTKQPP--RSSPNT 325
M5 TDPVLQVVYK---------SQGKESPGEEFSAEETEETFVKAETEKSDYDTPNYLLSPAA 367
M3 ETRAIYSIVLKLPGHSTILNSTKLPSSDNLQVPEEELGMVDLERKADKLQAQKSVDDGGS 419
M2 DSTSVSAVAS----------NMR--DDEITQDENTVSTSLG-----HSKDENSKQTCIRI 327
M4 ESSSGSATQN----------TKERPATELSTTEATTPAMPAPPLQPRALNPASRWSKIQI 344
. :
M1 VKRPTKK-----------------------------------GRDRAGKGQKPRGKEQLA 350
M5 AHRPKSQKCVAYKFRLVVKADGNQETNNGCHKVKIMPCPFPVAKEPSTKGLNPNPSHQMT 427
M3 FPKSFSKLPIQLESAVDTAKTSDVNSSVG---KSTATLPLSFKEATLAKRFALKTRSQIT 476
M2 GTKTPKSDSCTPTNTTVEVVGSSGQNGDE-------------KQNIVARKIVKMTK-QPA 373
M4 VTKQTGNECVT----AIEIVPAT-PAGMR-------------PAANVARKFASIARNQVR 386
: . : *
M1 KRKTFSLVKEKKAARTLSAILLAFILTWTPYNIMVLVSTFCKDCVPETLWELGYWLCYVN 410
M5 KRKRVVLVKERKAAQTLSAILLAFIITWTPYNIMVLVSTFCDKCVPVTLWHLGYWLCYVN 487
M3 KRKRMSLVKEKKAAQTLSAILLAFIITWTPYNIMVLVNTFCDSCIPKTFWNLGYWLCYIN 536
M2 KKKPP-PSREKKVTRTILAILLAFIITWAPYNVMVLINTFCAPCIPNTVWTIGYWLCYIN 432
M4 KKRQM-AARERKVTRTIFAILLAFILTWTPYNVMVLVNTFCQSCIPDTVWSIGYWLCYVN 445
*:: :*:*.::*: *******:**:***:***:.*** *:* *.* :******:*
M1 STINPMCYALCNKAFRDTFRLLLLCRWDKRRWRKIPKRPGS----VHRTPSRQC 460
M5 STVNPICYALCNRTFRKTFKMLLLCRWKKKKVEEKLYWQGN----SKLP----- 532
M3 STVNPVCYALCNKTFRTTFKMLLLCQCDKKKRRKQQYQQRQSVIFHKRAPEQAL 590
M2 STINPACYALCNATFKKTFKHLLMCHYKNIGATR-------------------- 466
M4 STINPACYALCNATFKKTFRHLLLCQYRNIGTAR-------------------- 479
**:** ****** :*: **: **:*: : .
Accession numbers of the muscarinic acetylcholine receptors used in the protein sequence alignment: NP_000729 cholinergic receptor, muscarinic 1; NP_001006633 cholinergic receptor, muscarinic 2; NP_000731 cholinergic receptor, muscarinic 3; NP_000732 cholinergic receptor, muscarinic 4; NP_036257 cholinergic receptor, muscarinic 5
Predicted General Properties of the M1 Muscarinic Acetylcholine Receptor (Homo sapiens) - Primary Sequence Analysis (with ProtParam)b
Number of amino acids: 460
Molecular weight: 51420.9
Theoretical pI: 9.45
Total number of negatively charged residues (Asp + Glu): 35
Total number of positively charged residues (Arg + Lys): 56
Atomic composition: Carbon C 2314 Hydrogen H 3659 Nitrogen N 625 Oxygen O 645 Sulfur S 27
Formula: C2314H3659N625O645S27
Total number of atoms: 7270
Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 96215 Abs 0.1% (=1 g/l) 1.871, assuming ALL Cys residues appear as half cystines Ext. coefficient 95340 Abs 0.1% (=1 g/l) 1.854, assuming NO Cys residues appear as half cystines
Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo).
Instability index: The instability index (II) is computed to be 48.30 This classifies the protein as unstable.
Aliphatic index: 86.54
Grand average of hydropathicity (GRAVY): -0.081
Bioinformatics References
a Higgins D., Thompson J., Gibson T. Thompson J. D., Higgins D. G., Gibson T. J.(1994). CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting,position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 22:4673-4680.
b Gasteiger E., Hoogland C., Gattiker A., Duvaud S., Wilkins M.R., Appel R.D., Bairoch A.; Protein Identification and Analysis Tools on the ExPASy Server;
(In) John M. Walker (ed): The Proteomics Protocols Handbook, Humana Press (2005). pp. 571-607 Full text - Copyright Humana Press.