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The Muscarinic Acetylcholine Receptors

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Sequence Alignment of the Muscarinic Acetylcholine Receptors (Homo sapiens) (with Clustalw)a

Isoform Sequence Alignment of Muscarinic Acetylcholine Receptors

M1  -------------MNTSAPPAVSPNITVLAPGKG-------------------------- 21
M5  -------MEGDSYHNATTVNGTPVNHQPLERHR--------------------------- 26
M3  MTLHNNSTTSPLFPNISSSWIHSPSDAGLPPGTVTHFGSYNVSRAAGNFSSPDGTTDDPL 60
M2  ------------MNNST----NSSNNSLALTSP--------------------------- 17
M4  ------------MANFTPVNGSSGNQSVRLVTSSSH------------------------ 24
                  * :     . .                                   

M1  ----PWQVAFIGITTGLLSLATVTGNLLVLISFKVNTELKTVNNYFLLSLACADLIIGTF 77
M5  ----LWEVITIAAVTAVVSLITIVGNVLVMISFKVNSQLKTVNNYYLLSLACADLIIGIF 82
M3  GGHTVWQVVFIAFLTGILALVTIIGNILVIVSFKVNKQLKTVNNYFLLSLACADLIIGVI 120
M2  --YKTFEVVFIVLVAGSLSLVTIIGNILVMVSIKVNRHLQTVNNYFLFSLACADLIIGVF 75
M4  NRYETVEMVFIATVTGSLSLVTVVGNILVMLSIKVNRQLQTVNNYFLFSLACADLIIGAF 84
          ::  *   :. ::* *: **:**::*:*** .*:*****:*:********** :

M1  SMNLYTTYLLMGHWALGTLACDLWLALDYVASNASVMNLLLISFDRYFSVTRPLSYRAKR 137
M5  SMNLYTTYILMGRWALGSLACDLWLALDYVASNASVMNLLVISFDRYFSITRPLTYRAKR 142
M3  SMNLFTTYIIMNRWALGNLACDLWLAIDYVASNASVMNLLVISFDRYFSITRPLTYRAKR 180
M2  SMNLYTLYTVIGYWPLGPVVCDLWLALDYVVSNASVMNLLIISFDRYFCVTKPLTYPVKR 135
M4  SMNLYTVYIIKGYWPLGAVVCDLWLALDYVVSNASVMNLLIISFDRYFCVTKPLTYPARR 144
    ****:* * : . *.** :.******:***.*********:*******.:*:**:* .:*

M1  TPRRAALMIGLAWLVSFVLWAPAILFWQYLVGERTVLAGQCYIQFLSQPIITFGTAMAAF 197
M5  TPKRAGIMIGLAWLISFILWAPAILCWQYLVGKRTVPLDECQIQFLSEPTITFGTAIAAF 202
M3  TTKRAGVMIGLAWVISFVLWAPAILFWQYFVGKRTVPPGECFIQFLSEPTITFGTAIAAF 240
M2  TTKMAGMMIAAAWVLSFILWAPAILFWQFIVGVRTVEDGECYIQFFSNAAVTFGTAIAAF 195
M4  TTKMAGLMIAAAWVLSFVLWAPAILFWQFVVGKRTVPDNQCFIQFLSNPAVTFGTAIAAF 204
    *.: *.:**. **::**:******* **:.** ***  .:* ***:*:. :*****:***

M1  YLPVTVMCTLYWRIYRETENRARELAALQGSETPGKG----------------------- 234
M5  YIPVSVMTILYCRIYRETEKRTKDLADLQGSDSVTKAEKRKPAHRALFRSCLRCPRPTLA 262
M3  YMPVTIMTILYWRIYKETEKRTKELAGLQASGTEAETENFVHPTGSS-RSCSSYELQQQS 299
M2  YLPVIIMTVLYWHISRASKSRIKKDKKEPVANQDPVSPSLVQ------------------ 237
M4  YLPVVIMTVLYIHISLASRSRVHKHRPEGPKEKKAKTLAFLK------------------ 246
    *:** :*  ** :*   :..* :.                                    

M1  ----GGSSSS------SERSQPGAEGSPETPPGRCCRCCRAPRLLQAYSWKEEEEED--- 281
M5  QRERNQASWS------SSRRSTSTTGKPSQATGPSANWAKAEQLTTCSSYPSSEDEDKPA 316
M3  MKRSNRRKYGRCHFWFTTKSWKPSSEQMDQDHSSSDSWNNNDAAASLENSASSDEEDIGS 359
M2  ----GRIVKP------NNNNMP---SSDDGLEHNKIQNGKAPRDPVTENCVQGEEKESSN 284
M4  ----SPLMKQ------SVKKPPPGEAAREELRNGKLEEAPPPALPPPPRPVA--DKDTSN 294
        .           . .         .                         :::   

M1  -EGSMESLTS---------SEGEE-PG---SEVVIKMPMVDPEAQAPTKQPP--RSSPNT 325
M5  TDPVLQVVYK---------SQGKESPGEEFSAEETEETFVKAETEKSDYDTPNYLLSPAA 367
M3  ETRAIYSIVLKLPGHSTILNSTKLPSSDNLQVPEEELGMVDLERKADKLQAQKSVDDGGS 419
M2  DSTSVSAVAS----------NMR--DDEITQDENTVSTSLG-----HSKDENSKQTCIRI 327
M4  ESSSGSATQN----------TKERPATELSTTEATTPAMPAPPLQPRALNPASRWSKIQI 344
                          .                          :          

M1   VKRPTKK-----------------------------------GRDRAGKGQKPRGKEQLA 350
M5   AHRPKSQKCVAYKFRLVVKADGNQETNNGCHKVKIMPCPFPVAKEPSTKGLNPNPSHQMT 427
M3   FPKSFSKLPIQLESAVDTAKTSDVNSSVG---KSTATLPLSFKEATLAKRFALKTRSQIT 476
M2   GTKTPKSDSCTPTNTTVEVVGSSGQNGDE-------------KQNIVARKIVKMTK-QPA 373
M4   VTKQTGNECVT----AIEIVPAT-PAGMR-------------PAANVARKFASIARNQVR 386
       :   .                                         :        *  

M1   KRKTFSLVKEKKAARTLSAILLAFILTWTPYNIMVLVSTFCKDCVPETLWELGYWLCYVN 410
M5   KRKRVVLVKERKAAQTLSAILLAFIITWTPYNIMVLVSTFCDKCVPVTLWHLGYWLCYVN 487
M3   KRKRMSLVKEKKAAQTLSAILLAFIITWTPYNIMVLVNTFCDSCIPKTFWNLGYWLCYIN 536
M2   KKKPP-PSREKKVTRTILAILLAFIITWAPYNVMVLINTFCAPCIPNTVWTIGYWLCYIN 432
M4   KKRQM-AARERKVTRTIFAILLAFILTWTPYNVMVLVNTFCQSCIPDTVWSIGYWLCYVN 445
     *::     :*:*.::*: *******:**:***:***:.***  *:* *.* :******:*

M1   STINPMCYALCNKAFRDTFRLLLLCRWDKRRWRKIPKRPGS----VHRTPSRQC 460
M5   STVNPICYALCNRTFRKTFKMLLLCRWKKKKVEEKLYWQGN----SKLP----- 532
M3   STVNPVCYALCNKTFRTTFKMLLLCQCDKKKRRKQQYQQRQSVIFHKRAPEQAL 590
M2   STINPACYALCNATFKKTFKHLLMCHYKNIGATR-------------------- 466
M4   STINPACYALCNATFKKTFRHLLLCQYRNIGTAR-------------------- 479
     **:** ****** :*: **: **:*:  :    .                     

Accession numbers of the muscarinic acetylcholine receptors used in the protein sequence alignment: NP_000729 cholinergic receptor, muscarinic 1; NP_001006633 cholinergic receptor, muscarinic 2; NP_000731 cholinergic receptor, muscarinic 3; NP_000732 cholinergic receptor, muscarinic 4; NP_036257 cholinergic receptor, muscarinic 5

Predicted General Properties of the M1 Muscarinic Acetylcholine Receptor  (Homo sapiens) - Primary Sequence Analysis (with ProtParam)b

Number of amino acids: 460

Molecular weight: 51420.9

Theoretical pI: 9.45

Total number of negatively charged residues (Asp + Glu): 35

Total number of positively charged residues (Arg + Lys): 56

Atomic composition: Carbon C 2314 Hydrogen H 3659 Nitrogen N 625 Oxygen O 645 Sulfur S 27

Formula: C2314H3659N625O645S27

Total number of atoms: 7270

Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 96215 Abs 0.1% (=1 g/l) 1.871, assuming ALL Cys residues appear as half cystines Ext. coefficient 95340 Abs 0.1% (=1 g/l) 1.854, assuming NO Cys residues appear as half cystines

Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo).

Instability index: The instability index (II) is computed to be 48.30 This classifies the protein as unstable.

Aliphatic index: 86.54

Grand average of hydropathicity (GRAVY): -0.081

Bioinformatics References

a Higgins D., Thompson J., Gibson T. Thompson J. D., Higgins D. G., Gibson T. J.(1994). CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting,position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 22:4673-4680.

b Gasteiger E., Hoogland C., Gattiker A., Duvaud S., Wilkins M.R., Appel R.D., Bairoch A.; Protein Identification and Analysis Tools on the ExPASy Server;
(In) John M. Walker (ed): The Proteomics Protocols Handbook, Humana Press (2005). pp. 571-607  Full text - Copyright Humana Press.

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