Sequence Alignment of the Matrix Metalloproteinase Protein (with Clustalw)a
Isoform Sequence Alignment (Homo sapiens)
of Matrix Metalloproteinases
MMP1 ---------------------------------------------------MHSFPPL-L 8
MMP8 ---------------------------------------------------MFSLKTLPF 9
MMP3 ------------------------------------------------------MKSLPI 6
MMP10 ------------------------------------------------------MMHLAF 6
MMP27 ------------------------------------------------------MKRLLL 6
MMP12 -------------------------------------------------------MKFLL 5
MMP13 ----------------------------------------------------MHPGVLAA 8
MMP7 ----------------------------------------------------MRLTVLCA 8
MMP20 ----------------------------------------------MKVLPASGLAVFLI 14
MMP2 --------------------------------------------MEALMARGALTGPLRA 16
MMP9 --------------------------------------------MSLWQP------LVLV 10
MMP26 ------------------------------------------------------MQLVIL 6
MMP19rasi-1 ------------------------------------------------------------
MMP28-1 ----------------------------------------------------MVARVGLL 8
MMP14 ----------------------------------MSPAPRPP-----------------R 9
MMP15 ----------------------------------MGSDPSAPGRPGWTGSLLGDREEAAR 26
MMP16-1 ----------------------------------MILLTFSTGR------RLDFVHHSGV 20
MMP16-2 ----------------------------------MILLTFSTGR------RLDFVHHSGV 20
MMP24 MPRSRGGRAAPGPPPPPPPPGQAPRWSRWRVPGRLLLLLLPALC------CLPGAARAAA 54
MMP17 ----------------------------------MRRRAARGPGPPPPGPGLSRLPLPLL 26
MMP25 ----------------------------------MRLR--------------LRLLALLL 12
MMP11 --------------------------------------------------------MAPA 4
MMP21 ---------------------------------MLAASIFRPTLLLCWLAAPWPTQPESL 27
MMP23B ------------------------------------------------------------
MMP1 LLLFWGVVSHSFPATLETQEQD-----VDLVQKYLEKYYNLKNDGRQVEKRRNSG-PVVE 62
MMP8 LLLLHVQISKAFPVS--SKEKN-----TKTVQDYLEKFYQLPSNQYQSTRKNGTN-VIVE 61
MMP3 LLLLCVAVCSAYPLDGAARGEDT---SMNLVQKYLENYYDLKKDVKQFVRRKDSG-PVVK 62
MMP10 LVLLCLPVCSAYPLSGAAKEEDS---NKDLAQQYLEKYYNLEKDVKQFRR-KDSN-LIVK 61
MMP27 LFLFFITFSSAFPLVRMTENEE----NMQLAQAYLNQFYSLEIEGNHLVQSKNRS-LIDD 61
MMP12 ILLLQATASGALPLNSSTSLEKN---NVLFGERYLEKFYGLEINKLPVTKMKYSGNLMKE 62
MMP13 FLFLSWTHCRALPLPSGGDEDDLSEEDLQFAERYLRSYYHPTNLAGILKENAASS--MTE 66
MMP7 VCLLPGSLALPLPQEAGGMSELQ----WEQAQDYLKRFYLYDS-------ETKNANSLEA 57
MMP20 MALKFSTAAPSLVAASPRTWRNN----YRLAQAYLDKYYTNKEGHQIGEMVARGSNSMIR 70
MMP2 LCLLGCLLSHAAAAPSPIIKFPG-DVAP-KTDKELAVQYLN--TFYGCPKESCNLFVLKD 72
MMP9 LLVLGCCFAAPRQRQSTLVLFPG-DLRTNLTDRQLAEEYLYRYGYTRVAEMRGESKSLGP 69
MMP26 RVTIFLPWCFAVPVPPAADHKG-----WDFVEGYFHQFFLTK---------KESPLLTQE 52
MMP19rasi-1 MNCQQLWLGFLLPMTVSGRVLGL---AEVAPVDYLSQYGYLQKPLEGSNNFKPED--ITE 55
MMP28-1 LRALQLLLWGHLDAQPAERGGQE---LRKEAEAFLEKYGYLNEQVPKAP--TSTR--FSD 61
MMP14 CLLLPLLTLGTALASLG-SAQSS----SFSPEAWLQQYGYLPPGDLRTHTQRSPQ-SLSA 63
MMP15 PRLLPLLLVLLGCLGLGVAAEDA----EVHAENWLRLYGYLPQPSRHMSTMRSAQ-ILAS 81
MMP16-1 FFLQT----LLWILCATVCGTEQ----YFNVEVWLQKYGYLPPTDPRMSVLRSAE-TMQS 71
MMP16-2 FFLQT----LLWILCATVCGTEQ----YFNVEVWLQKYGYLPPTDPRMSVLRSAE-TMQS 71
MMP24 AAAGAGNRAAVAVAVARADEAEA----PFAGQNWLKSYGYLLPYDSRASALHSAK-ALQS 109
MMP17 LLLALGTRGGCAAPAPAPRAEDL----SLGVE-WLSRFGYLPPADPTTGQLQTQE-ELSK 80
MMP25 LLLAPPAR------APKPSAQDV----SLGVD-WLTRYGYLPPPHPAQAQLQSPE-KLRD 60
MMP11 AWLRSAAARALLPPMLLLLLQPP----------PLLARALPPDAHHLHAERRGPQ----P 50
MMP21 FHSRDRSDLEPSPLRQAKPIADLHAAQRFLSRYGWSGVWAAWGPSPEGPPETPKGAALAE 87
MMP23B -----MGRGARVPSEAPGAGVER---------RWLGAALVALCLLPALVLLARLGAPAVP 46
MMP1 KLKQMQEFFGLKVTGKPDAETLKVMKQPRCGVPDV-----------------------AQ 99
MMP8 KLKEMQRFFGLNVTGKPNEETLDMMKKPRCGVPDS-----------------------GG 98
MMP3 KIREMQKFLGLEVTGKLDSDTLEVMRKPRCGVPDV-----------------------GH 99
MMP10 KIQGMQKFLGLEVTGKLDTDTLEVMRKPRCGVPDV-----------------------GH 98
MMP27 KIREMQAFFGLTVTGKLDSNTLEIMKTPRCGVPDV-----------------------GQ 98
MMP12 KIQEMQHFLGLKVTGQLDTSTLEMMHAPRCGVPDV-----------------------HH 99
MMP13 RLREMQSFFGLEVTGKLDDNTLDVMKKPRCGVPDV-----------------------GE 103
MMP7 KLKEMQKFFGLPITGMLNSRVIEIMQKPRCGVPDV-----------------------AE 94
MMP20 KIKELQAFFGLQVTGKLDQTTMNVIKKPRCGVPDV-----------------------AN 107
MMP2 TLKKMQKFFGLPQTGDLDQNTIETMRKPRCGNPDV-----------------------AN 109
MMP9 ALLLLQKQLSLPETGELDSATLKAMRTPRCGVPDL-----------------------GR 106
MMP26 TQTQLLQQFHRNGTDLLDMQMHALLHQPHCGVPDG-----------------------SD 89
MMP19rasi-1 ALRAFQEASELPVSGQLDDATRARMRQPRCGLEDP---------------------FNQK 94
MMP28-1 AIRAFQWVSQLPVSGVLDRATLRQMTRPRCGVTDTNSYAAWAERISDLFA-RHRTKMRRK 120
MMP14 AIAAMQKFYGLQVTGKADADTMKAMRRPRCGVPDKFGAEIKANVRR-------------- 109
MMP15 ALAEMQRFYGIPVTGVLDEETKEWMKRPRCGVPDQFGVRVKANLRRR------------R 129
MMP16-1 ALAAMQQFYGINMTGKVDRNTIDWMKKPRCGVPDQTRGSSKFHIRR-------------- 117
MMP16-2 ALAAMQQFYGINMTGKVDRNTIDWMKKPRCGVPDQTRGSSKFHIRR-------------- 117
MMP24 AVSTMQQFYGIPVTGVLDQTTIEWMKKPRCGVPDHPHLSR--RRRN-------------- 153
MMP17 AITAMQQFGGLEATGILDEATLALMKTPRCSLPDLPVLT--QARRR-------------- 124
MMP25 AIKVMQRFAGLPETGRMDPGTVATMRKPRCSLPDVLGVAGLVRRRR-------------- 106
MMP11 WHAALPSSPAPAPATQEAPRPASSLRPPRCGVPDPSDGLSARN---------------RQ 95
MMP21 AVRRFQRANALPASGELDAATLAAMNRPRCGVPDMRPPPPSAPPSPPGPPPRARSRRSPR 147
MMP23B AWSAAQGDVAALGLSAVPPTRVPGPLAPRRR----------------------------R 78
*:
MMP1 FVLTEGNPRWE--------------QTHLTYRIENYT--PDLPRADVDHAIEKAFQLWSN 143
MMP8 FMLTPGNPKWE--------------RTNLTYRIRNYT--PQLSEAEVERAIKDAFELWSV 142
MMP3 FRTFPGIPKWR--------------KTHLTYRIVNYT--PDLPKDAVDSAVEKALKVWEE 143
MMP10 FSSFPGMPKWR--------------KTHLTYRIVNYT--PDLPRDAVDSAIEKALKVWEE 142
MMP27 YG--YTLPGWR--------------KYNLTYRIINYT--PDMARAAVDEAIQEGLEVWSK 140
MMP12 FREMPGGPVWR--------------KHYITYRINNYT--PDMNREDVDYAIRKAFQVWSN 143
MMP13 YNVFPRTLKWS--------------KMNLTYRIVNYT--PDMTHSEVEKAFKKAFKVWSD 147
MMP7 YSLFPNSPKWT--------------SKVVTYRIVSYT--RDLPHITVDRLVSKALNMWGK 138
MMP20 YRLFPGEPKWK--------------KNTLTYRISKYT--PSMSSVEVDKAVEMALQAWSS 151
MMP2 YNFFPRKPKWD--------------KNQITYRIIGYT--PDLDPETVDDAFARAFQVWSD 153
MMP9 FQTFEGDLKWH--------------HHNITYWIQNYS--EDLPRAVIDDAFARAFALWSA 150
MMP26 TSISPGRCKWN--------------KHTLTYRIINYP--HDMKPSAVKDSIYNAVSIWSN 133
MMP19rasi-1 TLKYLLLGRWR--------------KKHLTFRILNLP--STLPPHTARAALRQAFQDWSN 138
MMP28-1 KRFAKQGNKWY--------------KQHLSYRLVNWP--EHLPEPAVRGAVRAAFQLWSN 164
MMP14 KRYAIQGLKWQ--------------HNEITFCIQNYT--PKVGEYATYEAIRKAFRVWES 153
MMP15 KRYALTGRKWN--------------NHHLTFSIQNYT--EKLGWYHSMEAVRRAFRVWEQ 173
MMP16-1 KRYALTGQKWQ--------------HKHITYSIKNVT--PKVGDPETRKAIRRAFDVWQN 161
MMP16-2 KRYALTGQKWQ--------------HKHITYSIKNVT--PKVGDPETRKAIRRAFDVWQN 161
MMP24 KRYALTGQKWR--------------QKHITYSIHNYT--PKVGELDTRKAIRQAFDVWQK 197
MMP17 -RQAPAPTKWN--------------KRNLSWRVRTFPRDSPLGHDTVRALMYYALKVWSD 169
MMP25 -RYALSGSVWK--------------KRTLTWRVRSFPQSSQLSQETVRVLMSYALMAWGM 151
MMP11 KRFVLSGGRWE--------------KTDLTYRILRFP--WQLVQEQVRQTMAEALKVWSD 139
MMP21 APLSLSRRGWQPRGYPDGGAAQAFSKRTLSWRLLGEALSSQLSVADQRRIVALAFRMWSE 207
MMP23B YTLTPARLRWD--------------HFNLTYRILSFPR-NLLSPRETRRALAAAFRMWSD 123
* ::: : . : . .. *
MMP1 VTPLTFTKVSEG--------QADIMISFVRGDHRDNSP------FDGPGGNLAHAFQPGP 189
MMP8 ASPLIFTRISQG--------EADINIAFYQRDHGDNSP------FDGPNGILAHAFQPGQ 188
MMP3 VTPLTFSRLYEG--------EADIMISFAVREHGDFYP------FDGPGNVLAHAYAPGP 189
MMP10 VTPLTFSRLYEG--------EADIMISFAVKEHGDFYS------FDGPGHSLAHAYPPGP 188
MMP27 VTPLKFTKISKG--------IADIMIAFRTRVHGRCPRY-----FDGPLGVLGHAFPPGP 187
MMP12 VTPLKFSKINTG--------MADILVVFARGAHGDFHA------FDGKGGILAHAFGPGS 189
MMP13 VTPLNFTRLHDG--------IADIMISFGIKEHGDFYP------FDGPSGLLAHAFPPGP 193
MMP7 EIPLHFRKVVWG--------TADIMIGFARGAHGDSYP------FDGPGNTLAHAFAPGT 184
MMP20 AVPLSFVRINSG--------EADIMISFENGDHGDSYP------FDGPRGTLAHAFAPGE 197
MMP2 VTPLRFSRIHDG--------EADIMINFGRWEHGDGYP------FDGKDGLLAHAFAPGT 199
MMP9 VTPLTFTRVYSR--------DADIVIQFGVAEHGDGYP------FDGKDGLLAHAFPPGP 196
MMP26 VTPLIFQQVQNG--------DADIKVSFWQWAHEDGWP------FDGPGGILGHAFLPNS 179
MMP19rasi-1 VAPLTFQEVQAG--------AADIRLSFHG-RQSSYCSN----TFDGPGRVLAHADIPEL 185
MMP28-1 VSALEFWEAPATG-------PADIRLTFFQGDHNDGLGN----AFDGPGGALAHAFLPRR 213
MMP14 ATPLRFREVPYAYIREGHEKQADIMIFFAEGFHGDSTP------FDGEGGFLAHAYFPGP 207
MMP15 ATPLVFQEVPYEDIRLRRQKEADIMVLFASGFHGDSSP------FDGTGGFLAHAYFPGP 227
MMP16-1 VTPLTFEEVPYSELENGK-RDVDITIIFASGFHGDSSP------FDGEGGFLAHAYFPGP 214
MMP16-2 VTPLTFEEVPYSELENGK-RDVDITIIFASGFHGDSSP------FDGEGGFLAHAYFPGP 214
MMP24 VTPLTFEEVPYHEIKSDR-KEADIMIFFASGFHGDSSP------FDGEGGFLAHAYFPGP 250
MMP17 IAPLNFHEVA--------GSAADIQIDFSKADHNDGYP------FDGPGGTVAHAFFPGH 215
MMP25 ESGLTFHEVDS-----PQGQEPDILIDFARAFHQDSYP------FDGLGGTLAHAFFPGE 200
MMP11 VTPLTFTEVHEG--------RADIMIDFARYWHGDDLP------FDGPGGILAHAFFPKT 185
MMP21 VTPLDFREDLAAPG-----AAVDIKLGFGRGRHLGCPR-----AFDGSGQEFAHAW---- 253
MMP23B VSPFSFREVAPEQ-------PSDLRIGFYPINHTDCLVSALHHCFDGPTGELAHAFFPPH 176
: * . *: : * : *** ..**
MMP1 G-IGGDAHFDEDERWTNN------------------------------------------ 206
MMP8 G-IGGDAHFDAEETWTNT------------------------------------------ 205
MMP3 G-INGDAHFDDDEQWTKD------------------------------------------ 206
MMP10 G-LYGDIHFDDDEKWTED------------------------------------------ 205
MMP27 G-LGGDTHFDEDENWTKD------------------------------------------ 204
MMP12 G-IGGDAHFDEDEFWTTH------------------------------------------ 206
MMP13 N-YGGDAHFDDDETWTSS------------------------------------------ 210
MMP7 G-LGGDAHFDEDERWTDGS----------------------------------------- 202
MMP20 G-LGGDTHFDNAEKWTMG------------------------------------------ 214
MMP2 G-VGGDSHFDDDELWTLGEGQVVRVKYGNADGEYCKFPFLFNGKEYNSCTDTGRSDGFLW 258
MMP9 G-IQGDAHFDDDELWSLGKGVVVPTRFGNADGAACHFPFIFEGRSYSACTTDGRSDGLPW 255
MMP26 G-NPGVVHFDKNEHWSAS------------------------------------------ 196
MMP19rasi-1 ----GSVHFDEDEFWTEGT----------------------------------------- 200
MMP28-1 ----GEAHFDQDERWSLSR----------------------------------------- 228
MMP14 N-IGGDTHFDSAEPWTVRNE---------------------------------------- 226
MMP15 G-LGGDTHFDADEPWTFSST---------------------------------------- 246
MMP16-1 G-IGGDTHFDSDEPWTLGNP---------------------------------------- 233
MMP16-2 G-IGGDTHFDSDEPWTLGNP---------------------------------------- 233
MMP24 G-IGGDTHFDSDEPWTLGNA---------------------------------------- 269
MMP17 HHTAGDTHFDDDEAWTFRSS---------------------------------------- 235
MMP25 HPISGDTHFDDEETWTFGSK---------------------------------------- 220
MMP11 H-REGDVHFDYDETWTIGDD---------------------------------------- 204
MMP21 --RLGDIHFDDDEHFTPPTS---------------------------------------- 271
MMP23B G----GIHFDDSEYWVLGPTRYSWKKG--------------------------------- 199
*** * :
MMP1 ------------------------------------------------------------
MMP8 ------------------------------------------------------------
MMP3 ------------------------------------------------------------
MMP10 ------------------------------------------------------------
MMP27 ------------------------------------------------------------
MMP12 ------------------------------------------------------------
MMP13 ------------------------------------------------------------
MMP7 ------------------------------------------------------------
MMP20 ------------------------------------------------------------
MMP2 CSTTYNFEKDGKYGFCPHEALFTMGGNAEGQPCKFPFRFQGTSYDSCTTEGRTDGYRWCG 318
MMP9 CSTTANYDTDDRFGFCPSERLYTQDGNADGKPCQFPFIFQGQSYSACTTDGRSDGYRWCA 315
MMP26 ------------------------------------------------------------
MMP19rasi-1 ------------------------------------------------------------
MMP28-1 ------------------------------------------------------------
MMP14 ------------------------------------------------------------
MMP15 ------------------------------------------------------------
MMP16-1 ------------------------------------------------------------
MMP16-2 ------------------------------------------------------------
MMP24 ------------------------------------------------------------
MMP17 ------------------------------------------------------------
MMP25 ------------------------------------------------------------
MMP11 ------------------------------------------------------------
MMP21 ------------------------------------------------------------
MMP23B ------------------------------------------------------------
MMP1 ------------------------------------------------------------
MMP8 ------------------------------------------------------------
MMP3 ------------------------------------------------------------
MMP10 ------------------------------------------------------------
MMP27 ------------------------------------------------------------
MMP12 ------------------------------------------------------------
MMP13 ------------------------------------------------------------
MMP7 ------------------------------------------------------------
MMP20 ------------------------------------------------------------
MMP2 TTEDYDRDKKYGFCPETAMSTVGG-NSEGAPCVFPFTFLGNKYESCTSAGRSDGKMWCAT 377
MMP9 TTANYDRDKLFGFCPTRADSTVMGGNSAGELCVFPFTFLGKEYSTCTSEGRGDGRLWCAT 375
MMP26 ------------------------------------------------------------
MMP19rasi-1 ------------------------------------------------------------
MMP28-1 ------------------------------------------------------------
MMP14 ------------------------------------------------------------
MMP15 ------------------------------------------------------------
MMP16-1 ------------------------------------------------------------
MMP16-2 ------------------------------------------------------------
MMP24 ------------------------------------------------------------
MMP17 ------------------------------------------------------------
MMP25 ------------------------------------------------------------
MMP11 ------------------------------------------------------------
MMP21 ------------------------------------------------------------
MMP23B ------------------------------------------------------------
MMP1 --------------FREYNLHRVAAHELGHSLGLSHSTDIGALMYPSYTF----SGDVQL 248
MMP8 --------------SANYNLFLVAAHEFGHSLGLAHSSDPGALMYPNYAFR--ETSNYSL 249
MMP3 --------------TTGTNLFLVAAHEIGHSLGLFHSANTEALMYPLYHSLT-DLTRFRL 251
MMP10 --------------ASGTNLFLVAAHELGHSLGLFHSANTEALMYPLYNSFT-ELAQFRL 250
MMP27 --------------GAGFNLFLVAAHEFGHALGLSHSNDQTALMFPNYVSL--DPRKYPL 248
MMP12 --------------SGGTNLFLTAVHEIGHSLGLGHSSDPKAVMFPTYKYV--DINTFRL 250
MMP13 --------------SKGYNLFLVAAHEFGHSLGLDHSKDPGALMFPIYTYT--GKSHFML 254
MMP7 --------------SLGINFLYAATHELGHSLGMGHSSDPNAVMYPTYGNG--DPQNFKL 246
MMP20 --------------TNGFNLFTVAAHEFGHALGLAHSTDPSALMYPTYKYK--NPYGFHL 258
MMP2 TANYDDDRKWGFCPDQGYSLFLVAAHEFGHAMGLEHSQDPGALMAPIYTYT----KNFRL 433
MMP9 TSNFDSDKKWGFCPDQGYSLFLVAAHEFGHALGLDHSSVPEALMYPMYRFT----EGPPL 431
MMP26 --------------DTGYNLFLVATHEIGHSLGLQHSGNQSSIMYPTYWYH--DPRTFQL 240
MMP19rasi-1 --------------YRGVNLRIIAAHEVGHALGLGHSRYSQALMAPVYEGY---RPHFKL 243
MMP28-1 --------------RRGRNLFVVLAHEIGHTLGLTHSPAPRALMAPYYKRL---GRDALL 271
MMP14 -------------DLNGNDIFLVAVHELGHALGLEHSSDPSAIMAPFYQ--WMDTENFVL 271
MMP15 -------------DLHGNNLFLVAVHELGHALGLEHSSNPNAIMAPFYQ--WKDVDNFKL 291
MMP16-1 -------------NHDGNDLFLVAVHELGHALGLEHSNDPTAIMAPFYQ--YMETDNFKL 278
MMP16-2 -------------NHDGNDLFLVAVHELGHALGLEHSNDPTAIMAPFYQ--YMETDNFKL 278
MMP24 -------------NHDGNDLFLVAVHELGHALGLEHSSDPSAIMAPFYQ--YMETHNFKL 314
MMP17 -------------DAHGMDLFAVAVHEFGHAIGLSHVAAAHSIMRPYYQGPVGDPLRYGL 282
MMP25 -------------DGEGTDLFAVAVHEFGHALGLGHSSAPNSIMRPFYQGPVGDPDKYRL 267
MMP11 ---------------QGTDLLQVAAHEFGHVLGLQHTTAAKALMSAFYTFR----YPLSL 245
MMP21 --------------DTGISLLKVAVHEIGHVLGLPHTYRTGSIMQPNYIPQ---EPAFEL 314
MMP23B --------------VWLTDLVHVAAHEIGHALGLMHSQHGRALMHLNATLR----GWKAL 241
.: .**.** :*: * ::* *
MMP1 AQDDIDGIQAIYG-RSQNPVQ--------------------------------------- 268
MMP8 PQDDIDGIQAIYG-LSSNPIQ--------------------------------------- 269
MMP3 SQDDINGIQSLYGPPPDSPETPLV------------------------------------ 275
MMP10 SQDDVNGIQSLYGPPPASTEEPLV------------------------------------ 274
MMP27 SQDDINGIQSIYGGLPKEPAK--------------------------------------- 269
MMP12 SADDIRGIQSLYGDPKENQRLP-------------------------------------- 272
MMP13 PDDDVQGIQSLYGPGDEDPNP--------------------------------------- 275
MMP7 SQDDIKGIQKLYGKR--------------------------------------------- 261
MMP20 PKDDVKGIQALYGPRKVFLGKPTL------------------------------------ 282
MMP2 SQDDIKGIQELYGASPDIDLG--------------------------------------- 454
MMP9 HKDDVNGIRHLYGPRPEPEPRPPTTTTPQPTAPPTVCPTGPPTVHPS---ERPTAGPTGP 488
MMP26 SADDIQRIQHLYG----------------------------------------------- 253
MMP19rasi-1 HPDDVAGIQALYGK---KSPVIRD--------------------------EEEEETELPT 274
MMP28-1 SWDDVLAVQSLYGKPLGGSVAVQL--------------------------PGKLFTDFET 305
MMP14 PDDDRRGIQQLYGGESGFPTKMPPQPRTTSR-PSVPD--------------KPKNP---- 312
MMP15 PEDDLRGIQQLYGTPDGQPQPTQPLPTVTPRRPGRPDHRPPRPPQPPPPGGKPERPPKPG 351
MMP16-1 PNDDLQGIQKIYGPPDKIPPPTRPLPTVPPHRSIPPADPRKNDRP------KPPRPPTG- 331
MMP16-2 PNDDLQGIQKIYGPPDKIPPPTRPLPTVPPHRSIPPADPRKNDRP------KPPRPPTG- 331
MMP24 PQDDLQGIQKIYGPPAEPLEPTRPLPTLPVRRIHSPSERKHERQP------RPPRPPLGD 368
MMP17 PYEDKVRVWQLYGVRESVSPTAQPEEPPLLPEP-PDNRSS-----------APPRK---- 326
MMP25 SQDDRDGLQQLYG-KAPQTPYDKPTRKPLAPPPQPPASPT-----------HSPSF---- 311
MMP11 SPDDCRGVQHLYGQPWPTVTSRTPALGPQAG----------------------------- 276
MMP21 DWSDRKAIQKLYGSCEGSFDTAFD------------------------------------ 338
MMP23B SQDELWGLHRLYGCLDRLFVCAS------------------------------------- 264
.: : :**
MMP1 ---------PIGPQTPKACDSKLT--------FDAITTIRGEVMFFKDRFYMRTNPFYPE 311
MMP8 ---------PTGPSTPKPCDPSLT--------FDAITTLRGEILFFKDRYFWRRHPQLQR 312
MMP3 ----PTEPVPPEPGTPANCDPALS--------FDAVSTLRGEILIFKDRHFWRKSLRKLE 323
MMP10 ----PTKSVPSGSEMPAKCDPALS--------FDAISTLRGEYLFFKDRYFWRRSHWNPE 322
MMP27 ---------PKEPTIPHACDPDLT--------FDAITTFRREVMFFKGRHLWRIYYDITD 312
MMP12 ---------NPDNSEPALCDPNLS--------FDAVTTVGNKIFFFKDRFFWLKVSERPK 315
MMP13 ----------KHPKTPDKCDPSLS--------LDAITSLRGETMIFKDRFFWRLHPQQVD 317
MMP7 --------------------SNS-----------------------------RKK----- 267
MMP20 ----PHAP-HHKPSIPDLCDSSSS--------FDAVTMLGKELLLFKDRIFWRRQVHLRT 329
MMP2 --TGPT--PTLGPVTPEICKQD--------IVFDGIAQIRGEIFFFKDRFIWRTVTPR-D 501
MMP9 PSAGPTGPPTAGPSTATTVPLSPVDDACNVNIFDAIAEIGNQLYLFKDGKYWRFSEGRGS 548
MMP26 ----------------EKCSSDIP------------------------------------ 261
MMP19rasi-1 VPPVPTE----PSPMPDPCSSELD---------AMMLGPRGKTYAFKGDYVWTVSDSGPG 321
MMP28-1 WDSYSPQGRRPETQGPKYCHSSFD---------AITVDRQQQLYIFKGSHFWEVAADGNV 356
MMP14 ------------TYGPNICDGNFD----------TVAMLRGEMFVFKERWFWRVRNNQ-V 349
MMP15 PPVQPRATERPDQYGPNICDGDFD----------TVAMLRGEMFVFKGRWFWRVRHNR-V 400
MMP16-1 RPSYP-------GAKPNICDGNFN----------TLAILRREMFVFKDQWFWRVRNNR-V 373
MMP16-2 RPSYP-------GAKPNICDGNFN----------TLAILRREMFVFKDQWFWRVRNNR-V 373
MMP24 RPSTP-------GTKPNICDGNFN----------TVALFRGEMFVFKDRWFWRLRNNR-V 410
MMP17 -------------DVPHRCSTHFD----------AVAQIRGEAFFFKGKYFWRLTRDRHL 363
MMP25 -------------PIPDRCEGNFD----------AIANIRGETFFFKGPWFWRLQPSGQL 348
MMP11 -IDTNEIAPLEPDAPPDACEASFD----------AVSTIRGELFFFKAGFVWRLRGGQLQ 325
MMP21 --------------WIRKERNQYG-----------EVMVRFSTYFFRNSWYWLYENRNNR 373
MMP23B ------------WARRGFCDARRR----------LMKRLCPSSCDFCYEFPFPTVATTPP 302
MMP1 ---VELNFISVFWPQLPN---GLEAAYEFADRDEVRFFKGNKYWAVQGQNVLHGYPKDIY 365
MMP8 ---VEMNFISLFWPSLPT---GIQAAYEDFDRDLIFLFKGNQYWALSGYDILQGYPKDIS 366
MMP3 ---PELHLISSFWPSLPS---GVDAAYEVTSKDLVFIFKGNQFWAIRGNEVRAGYPRGIH 377
MMP10 ---PEFHLISAFWPSLPS---YLDAAYEVNSRDTVFIFKGNEFWAIRGNEVQAGYPRGIH 376
MMP27 ---VEFELIASFWPSLPA---DLQAAYE-NPRDKILVFKDENFWMIRGYAVLPDYPKSIH 365
MMP12 ---TSVNLISSLWPTLPS---GIEAAYEIEARNQVFLFKDDKYWLISNLRPEPNYPKSIH 369
MMP13 ---AELFLTKSFWPELPN---RIDAAYEHPSHDLIFIFRGRKFWALNGYDILEGYPKKIS 371
MMP7 ------------------------------------------------------------
MMP20 G--IRPSTITSSFPQLMS---NVDAAYEVAERGTAYFFKGPHYWITRGFQ-MQGPPRTIY 383
MMP2 K-PMGPLLVATFWPELPE---KIDAVYEAPQEEKAVFFAGNEYWIYSASTLERGYPKPLT 557
MMP9 R-PQGPFLIADKWPALPR---KLDSVFEERLSKKLFFFSGRQVWVYTGASVLG--PRRLD 602
MMP26 ------------------------------------------------------------
MMP19rasi-1 ---PLFR-VSALWEGLPG---NLDAAVYSPRTQWIHFFKGDKVWRYINFKMSPGFPKKLN 374
MMP28-1 ---SEPRPLQERWVGLPP---NIEAAAVSLNDGDFYFFKGGRCWRFRGPKPVWGLPQLCR 410
MMP14 M-DGYPMPIGQFWRGLPA---SINTAYERK-DGKFVFFKGDKHWVFDEASLEPGYPKHIK 404
MMP15 L-DNYPMPIGHFWRGLPG---DISAAYERQ-DGRFVFFKGDRYWLFREANLEPGYPQPLT 455
MMP16-1 M-DGYPMQITYFWRGLPP---SIDAVYENS-DGNFVFFKGNKYWVFKDTTLQPGYPHDLI 428
MMP16-2 M-DGYPMQITYFWRGLPP---SIDAVYENS-DGNFVFFK--------------------- 407
MMP24 Q-EGYPMQIEQFWKGLPA---RIDAAYERA-DGRFVFFKGDKYWVFKEVTVEPGYPHSLG 465
MMP17 V-SLQPAQMHRFWRGLPLHLDSVDAVYERTSDHKIVFFKGDRYWVFKDNNVEEGYPRPVS 422
MMP25 V-SPRPARLHRFWEGLPAQVRVVQAAYARHRDGRILLFSGPQFWVFQDRQLEGG-ARPLT 406
MMP11 P--GYPALASRHWQGLPS---PVDAAFEDA-QGHIWFFQGAQYWVYDGEKPVLGPAPLTE 379
MMP21 TRYGDPIQILTGWPGIPTHNIDAFVHIWTWKRDERYFFQGNQYWRYDSDKDQALTEDEQG 433
MMP23B P----PRTKTRLVPEGRN--------VTFRCGQKILHKKGKVYWYKDQEPLEFSYPGYLA 350
MMP1 S------SFGFPRTVKHIDAALSEENTGKTYFFVANKYWRYDEYKRSMDPGYPKMIAHDF 419
MMP8 N-------YGFPSSVQAIDAAVFYR--SKTYFFVNDQFWRYDNQRQFMEPGYPKSISGAF 417
MMP3 T-------LGFPPTVRKIDAAISDKEKNKTYFFVEDKYWRFDEKRNSMEPGFPKQIAEDF 430
MMP10 T-------LGFPPTIRKIDAAVSDKEKKKTYFFAADKYWRFDENSQSMEQGFPRLIADDF 429
MMP27 T-------LGFPGRVKKIDAAVCDKTTRKTYFFVGIWCWRFDEMTQTMDKGFPQRVVKHF 418
MMP12 S-------FGFPNFVKKIDAAVFNPRFYRTYFFVDNQYWRYDERRQMMDPGYPKLITKNF 422
MMP13 E-------LGLPKEVKKISAAVHFEDTGKTLLFSGNQVWRYDDTNHIMDKDYPRLIEEDF 424
MMP7 ------------------------------------------------------------
MMP20 D-------FGFPRHVQQIDAAVYLREPQKTLFFVGDEYYSYDERKRKMEKDYPKNTEEEF 436
MMP2 S-------LGLPPDVQRVDAAFNWSKNKKTYIFAGDKFWRYNEVKKKMDPGFPKLIADAW 610
MMP9 K-------LGLGADVAQVTGALRSGRGK-MLLFSGRRLWRFDVKAQMVDPRSASEVDRMF 654
MMP26 ------------------------------------------------------------
MMP19rasi-1 R------------VEPNLDAALYWPLNQKVFLFKGSGYWQWDELARTDFSSYPKPIKGLF 422
MMP28-1 AGG----------LPRHPDAALFFPPLRRLILFKGARYYVLARGGLQVEPYYPRSLQ-DW 459
MMP14 E-------LGRGLPTDKIDAALFWMPNGKTYFFRGNKYYRFNEELRAVDSEYPKNIK-VW 456
MMP15 S-------YGLGIPYDRIDTAIWWEPTGHTFFFQEDRYWRFNEETQRGDPGYPKPIS-VW 507
MMP16-1 T-------LGSGIPPHGIDSAIWWEDVGKTYFFKGDRYWRYSEEMKTMDPGYPKPIT-VW 480
MMP16-2 --------------------------------VKGD------------------------ 411
MMP24 E-------LGSCLPREGIDTALRWEPVGKTYFFKGERYWRYSEERRATDPGYPKPIT-VW 517
MMP17 D-------FSLP-PGG-IDAAFSWAHNDRTYFFKDQLYWRYDDHTRHMDPGYPAQSP-LW 472
MMP25 E-------LGLP-PGEEVDAVFSWPQNGKTYLVRGRQYWRYDEAAARPDPGYPRDLS-LW 457
MMP11 LG---------LVRFPVHAALVWGPEKNKIYFFRGRDYWRFHPSTRRVDSPVPRRAT-DW 429
MMP21 KSYPKLISEGFPGIPSPLDTAFYDRRQKLIYFFKESLVFAFDVNRNRVLNSYPKRITEVF 493
MMP23B LG-----EAHLSIIANAVNEGTYTCVVRRQQRVLTTYSWRVRVRG--------------- 390
MMP1 PGIGHK------VDAVFMKDG--FFYFFHGTRQYKFDPKT--KRILTLQK--------AN 461
MMP8 PGIESK------VDAVFQQEH--FFHVFSGPRYYAFDLIA--QRVTRVAR--------GN 459
MMP3 PGIDSK------IDAVFEEFG--FFYFFTGSSQLEFDPNA--KKVTHTLK--------SN 472
MMP10 PGVEPK------VDAVLQAFG--FFYFFSGSSQFEFDPNA--RMVTHILK--------SN 471
MMP27 PGISIR------VDAAFQYKG--FFFFSRGSKQFEYDIKT--KNITRIMR--------TN 460
MMP12 QGIGPK------IDAVFYSKNK-YYYFFQGSNQFEYDFLL--QRITKTLK--------SN 465
MMP13 PGIGDK------VDAVYEKNG--YIYFFNGPIQFEYSIWS--NRIVRVMP--------AN 466
MMP7 ------------------------------------------------------------
MMP20 SGVNGQ------IDAAVELNG--YIYFFSGPKTYKYDTEK--EDVVSVVK--------SS 478
MMP2 NAIPDN------LDAVVDLQGGGHSYFFKGAYYLKLENQS-LKSV-KFGS--------IK 654
MMP9 PGVP--------LDTHDVFQYREKAYFCQDRFYWRVSSRSELNQVDQVGY--------VT 698
MMP26 ------------------------------------------------------------
MMP19rasi-1 TGVPNQ------PSAAMSWQDG-RVYFFKGKVYWRLNQQLRVEKGYPRNI--------SH 467
MMP28-1 GGIPEE------VSGALPRPDG-SIIFFRDDRYWRLDQAKLQATTSGRWA--------TE 504
MMP14 EGIPES------PRGSFMGSDEVFTYFYKGNKYWKFNNQKLKVEPGYPKS--------AL 502
MMP15 QGIPAS------PKGAFLSNDAAYTYFYKGTKYWKFDNERLRMEPGYPKS--------IL 553
MMP16-1 KGIPES------PQGAFVHKENGFTYFYKGKEYWKFNNQILKVEPGYPRS--------IL 526
MMP16-2 ---------------TLSIIQDGWLYKY----HWKW---ILEQRQSVP------------ 437
MMP24 KGIPQA------PQGAFISKEGYYTYFYKGRDYWKFDNQKLSVEPGYPRN--------IL 563
MMP17 RGVPST------LDDAMRWSDG-ASYFFRGQEYWKVLDGELEVAPGYPQS--------TA 517
MMP25 EGAPPS------PDDVTVSNAG-DTYFFKGAHYWRFPKNSIKTEPDAPQP--------MG 502
MMP11 RGVPSE------IDAAFQDADG-YAYFLRGRLYWKFDPVKVKALEGFPRL--------VG 474
MMP21 PAVIPQNHPFRNIDSAYYSYAYNSIFFFKGNAYWKVVNDKDKQQNSWLPANGLFPKKFIS 553
MMP23B ------------------------------------------------------------
MMP1 -SWFNCRKN--------------------------------------------------- 469
MMP8 -KWLNCRYG--------------------------------------------------- 467
MMP3 -SWLNC------------------------------------------------------ 477
MMP10 -SWLHC------------------------------------------------------ 476
MMP27 -TWFQCKEPKNSSF----------------------------------GFDINKEKAHSG 485
MMP12 -SWFGC------------------------------------------------------ 470
MMP13 -SILWC------------------------------------------------------ 471
MMP7 ------------------------------------------------------------
MMP20 -SWIGC------------------------------------------------------ 483
MMP2 SDWLGC------------------------------------------------------ 660
MMP9 YDILQCPED--------------------------------------------------- 707
MMP26 ------------------------------------------------------------
MMP19rasi-1 N-WMHCRPRTIDTT----------------------------------PSGGNTTPSGTG 492
MMP28-1 LPWMGCWHANSGSA----------------------------------LF---------- 520
MMP14 RDWMGCPS--------------------GGRP----------------DEGTEEETEVII 526
MMP15 RDFMGCQEHVEPGPRWPDVARPPFNPHGGAEPGADSAEGDVGDGDGDFGAGVNKDGGSRV 613
MMP16-1 KDFMGCDG-------------------PTDRV----------------KEGHSPPDDVDI 551
MMP16-2 ---------------------------VLSRQ----------------TEKHKTYEELSS 454
MMP24 RDWMGCNQK------------------EVERR----------------KERRLPQDDVDI 589
MMP17 RDWLVCGDSQA----------------DGSVAAGVDA-----------AEGPRAPPGQHD 550
MMP25 PNWLDCPAP---------------------------------------SSGPRAPRPPKA 523
MMP11 PDFFGCAEPANTFL---------------------------------------------- 488
MMP21 EKWFDVCDVHISTLNM-------------------------------------------- 569
MMP23B ------------------------------------------------------------
MMP1 --------------------------------------------------------
MMP8 --------------------------------------------------------
MMP3 --------------------------------------------------------
MMP10 --------------------------------------------------------
MMP27 GIKILYHKSLSLFIFGIVHLLKNTSIYQ---------------------------- 513
MMP12 --------------------------------------------------------
MMP13 --------------------------------------------------------
MMP7 --------------------------------------------------------
MMP20 --------------------------------------------------------
MMP2 --------------------------------------------------------
MMP9 --------------------------------------------------------
MMP26 --------------------------------------------------------
MMP19rasi-1 ITLDTTLSATETTFEY---------------------------------------- 508
MMP28-1 --------------------------------------------------------
MMP14 IEVDEEGGGAVSAAAVVLPVLLLLLVLAVGLAVFFFRRHGTPRRLLYCQRSLLDKV 582
MMP15 VVQMEEVARTVNVVMVLVPLLLLLCVLGLTYALVQMQRKGAPRVLLYCKRSLQEWV 669
MMP16-1 VIKLDNTASTVKAIAIVIPCILALCLLVLVYTVFQFKRKGTPRHILYCKRSMQEWV 607
MMP16-2 ITY----------------------------------------------------- 457
MMP24 MVTINDVPGSVNAVAVVIPCILSLCILVLVYTIFQFKNKTGPQPVTYYKRPVQEWV 645
MMP17 QSRSEDGYEVCSCTSGASSPPGAPGPLVAATMLLLLPPLSPGALWTAAQALTL--- 603
MMP25 TPVS----ETCDCQCELN---QAAGRWPAPIPLLLLPLLVGGVASR---------- 562
MMP11 --------------------------------------------------------
MMP21 --------------------------------------------------------
MMP23B --------------------------------------------------------
Accession numbers of the matrix metalloproteinases used in the protein sequence alignment: NP_002412 matrix metalloproteinase 1; NP_004521 matrix metalloproteinase 2; NP_002413 matrix metalloproteinase 3; NP_002414 matrix metalloproteinase
7; NP_002415 matrix metalloproteinase 8; NP_004985 matrix
metalloproteinase 9; NP_002416 matrix metalloproteinase 10; NP_005931
matrix metalloproteinase 11; NP_002417 matrix metalloproteinase 12;
NP_002418 matrix metalloproteinase 13; NP_004986 matrix metalloproteinase
14; NP_002419.1| matrix metalloproteinase 15; NP_005932 matrix
metalloproteinase 16 isoform 1; NP_072086 matrix metalloproteinase 16
isoform 2; NP_057239 matrix metalloproteinase 17; NP_002420 matrix
metalloproteinase 19 isoform rasi-1; NP_004762 matrix metalloproteinase 20
preproprotein; NP_671724 matrix metalloproteinase 21; NP_008914 matrix
metalloproteinase 23B; NP_006681 matrix metalloproteinase 24; NP_071913
matrix metalloproteinase 25; NP_068573 matrix metalloproteinase 26;
NP_071405 matrix metalloproteinase 27; NP_077278 matrix metalloproteinase
28 isoform 1
Predicted General Properties of the Matrix Metalloproteinase 1 (MMP1) Protein (Homo sapiens) - Primary Sequence Analysis (with ProtParam)b
Number of amino acids: 469
Molecular weight: 54006.9
Theoretical pI: 6.47
Total number of negatively charged residues (Asp + Glu): 58
Total number of positively charged residues (Arg + Lys): 54
Atomic composition: Carbon C 2458 Hydrogen H 3666 Nitrogen N 656 Oxygen O 699 Sulfur S 13
Formula: C2458H3666N656O699S13
Total number of atoms: 7492
Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 76905 Abs 0.1% (=1 g/l) 1.424, assuming ALL Cys residues appear as half cystines Ext. coefficient 76780 Abs 0.1% (=1 g/l) 1.422, assuming NO Cys residues appear as half cystines
Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo).
Instability index: The instability index (II) is computed to be 35.46 This classifies the protein as stable.
Aliphatic index: 65.27
Grand average of hydropathicity (GRAVY): -0.572
Bioinformatics References
a Higgins D., Thompson J., Gibson T. Thompson J. D., Higgins D. G., Gibson T. J.(1994). CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting,position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 22:4673-4680.
b Gasteiger E., Hoogland C., Gattiker A., Duvaud S., Wilkins M.R., Appel R.D., Bairoch A.; Protein Identification and Analysis Tools on the ExPASy Server;
(In) John M. Walker (ed): The Proteomics Protocols Handbook, Humana Press (2005). pp. 571-607 Full text - Copyright Humana Press.
