Macromolecule Insights - Protein Bioinformatics Analysis

Privacy Policy

The Lysozyme Protein

Sequence Alignment of the Lyoszyme Protein (with Clustalw)^a

Species Sequence Alignment of Lysozyme

Human           --------MKALIVLGLVLLSVTVQG---------KVFERCELARTLKRLGMDGYRGISL 43
Chimpanzee      --------MKALIVLGLVLLSVTVQG---------KVFERCELARTLKRLGMDGYRGISL 43
Mouse           --------MKALLTLGLLLLSVTAQA---------KVYNRCELARILKRNGMDGYRGVKL 43
Rat             --------MKALLVLGFLLLSASVQA---------KIYERCEFARTLKRNGMSGYYGVSL 43
Pig             --------MKTLLVLALLLLSVSVQA---------KVYDRCEFARILKKSGMDGYRGVSL 43
Zebrafish       --------MRLAVVFLCLAWMSSCES---------KTLGRCDVYKIFKNEGLDGFEGFSI 43
Chicken         MLGKNDPMCLVLVLLGLTALLGICQGGTGCYGSVSRIDTTGASCRTAKPEGLS-YCGVRA 59
                            : :         :.         :        :  *  *:. : *.  

Human           ANWMCLAKWESG-----------------------------YNTRATNYNAGDRSTDYGI 74
Chimpanzee      ANWMCLAKWESG-----------------------------YNTRATNYNAGDRSTDYGI 74
Mouse           ADWVCLAQHESN-----------------------------YNTRATNYNRGDRSTDYGI 74
Rat             ADWVCLAQHESN-----------------------------YNTQARNYNPGDQSTDYGI 74
Pig             ANWVCLAKWESN-----------------------------FNTKATNYNPGSQSTDYGI 74
Zebrafish       GNYVCTAYWESR-----------------------------FKTHRVR--SADTGKDYGI 72
Chicken         SRTIAERDLGSMNKYKVLIKRVGEALCIEPAVIAGIISRESHAGKILKNGWGDRGNGFGL 119
                .  :.     *                              .  :  .   .. ...:*:

Human           FQINSRYWCNDGKTPGAVNACH-LSCSALLQDNIADAVACAKRVVRDPQGIRAWVAWRNR 133
Chimpanzee      FQINSRYWCNDGKTPGAVNACH-LSCSALLQDNIADAVACAKRVVRDPQGIRAWVAWRNR 133
Mouse           FQINSRYWCNDGKTPRSKNACG-INCSALLQDDITAAIQCAKRVVRDPQGIRAWVAWRTQ 133
Rat             FQINSRYWCNDGKTPRAKNACG-IPCSALLQDDITQAIQCAKRVVRDPQGIRAWVAWQRH 133
Pig             FQINSRYWCNDGKTPKAVNACH-ISCKVLLDDDLSQDIECAKRVVRDPQGIKAWVAWKAH 133
Zebrafish       FQINSFKWCDDG-TPGGKNLCK-VACSDLLNDDLKASVGCAKLIVK-MDGLKSWETWDSY 129
Chicken         MQVDKRYHKIEGTWNGEAHIRQGTRILIDMVKKIQRKFPRWTRDQQLKGGISAYNAGVGN 179
                :*::.     :*      :          : ..:   .   .   :   *: :: :    

Human           CQNRD-------VRQYVQGCGV---------- 148
Chimpanzee      CQNRD-------VRQYVQGCGV---------- 148
Mouse           CQNRD-------LSQYIRNCGV---------- 148
Rat             CKNRD-------LSGYIRNCGV---------- 148
Pig             CQNKD-------VSQYIRGCKL---------- 148
Zebrafish       CNGRK-------MSRWVKGCEQRKQSLRA--- 151
Chicken         VRSYERMDIGTLHDDYSNDVVARAQYFKQHGY 211
                 .. .          : ..             

Accession Numbers of the lysozyme sequences used in the protein sequence alignment:  Human - NP_000230; Chimpanzee - NP_001009073; Mouse - NP_038618; Rat - NP_036903; Pig - NP_999557; Chicken - NP_001001470; Zebrafish - NP_631919

Predicted General Properties of the Lyoszyme Protein  (Homo sapiens) - Primary Sequence Analysis (with ProtParam)^b

Number of amino acids: 148

Molecular weight: 16537.0

Theoretical pI: 9.38

Total number of negatively charged residues (Asp + Glu): 11

Total number of positively charged residues (Arg + Lys): 20

Atomic composition: Carbon C 719 Hydrogen H 1146 Nitrogen N 220 Oxygen O 207 Sulfur S 11

Formula: C₇₁₉H₁₁₄₆N₂₂₀O₂₀₇S₁₁

Total number of atoms: 2303

Extinction coefficients: Extinction coefficients are in units of M^-1 cm^-1, at 280 nm measured in water. Ext. coefficient 36940 Abs 0.1% (=1 g/l) 2.234, assuming ALL Cys residues appear as half cystines Ext. coefficient 36440 Abs 0.1% (=1 g/l) 2.204, assuming NO Cys residues appear as half cystines

Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo).

Instability index: The instability index (II) is computed to be 27.71 This classifies the protein as stable.

Aliphatic index: 85.68

Grand average of hydropathicity (GRAVY): -0.195

Bioinformatics References

^a Higgins D., Thompson J., Gibson T. Thompson J. D., Higgins D. G., Gibson T. J.(1994). CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting,position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 22:4673-4680.

^b Gasteiger E., Hoogland C., Gattiker A., Duvaud S., Wilkins M.R., Appel R.D., Bairoch A.; Protein Identification and Analysis Tools on the ExPASy Server;
(In) John M. Walker (ed): The Proteomics Protocols Handbook, Humana Press (2005). pp. 571-607  Full text - Copyright Humana Press.