Macromolecule Insights - Protein Bioinformatics Analysis

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The Insulin Protein

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Sequence Alignment of the Insulin Protein (with Clustalw) a

Species Sequence Alignment of Insulin

Human      MALWMRLLPLLALLALWGPDPAAAFVNQHLCGSHLVEALYLVCGERGFFYTPKTRREAED 60
Chimpanzee MALWMRLLPLLVLLALWGPDPASAFVNQHLCGSHLVEALYLVCGERGFFYTPKTRREAED 60
Mouse      MALLVHFLPLLALLALWEPKPTQAFVKQHLCGPHLVEALYLVCGERGFFYTPKSRREVED 60
Rabbit     MASLAALLPLLALLVLCRLDPAQAFVNQHLCGSHLVEALYLVCGERGFFYTPKSRREVEE 60
Frog       MALWMQCLPLVLVLFFSTPN-TEALVNQHLCGSHLVEALYLVCGDRGFFYYPKVKRDMEQ 59
Zebrafish  MAVWLQAGALLVLLVVSSVS-TNPGTPQHLCGSHLVDALYLVCGPTGFFYNPK--RDVEP 57
           **      .*: :* .   . : . . *****.***:*******  **** **  *: * 

Human      LQVG---QVELGGGPGAGSLQPLALEGSLQKRGIVEQCCTSICSLYQLENYCN 110
Chimpanzee LQVG---QVELGGGPGAGSLQPLALEGSLQKRGIVEQCCTSICSLYQLENYCN 110
Mouse      PQVE---QLELGGSPGD--LQTLALEVARQKRGIVDQCCTSICSLYQLENYCN 108
Rabbit     LQVG---QAELGGGPGAGGLQPSALELALQKRGIVEQCCTSICSLYQLENYCN 110
Frog       ALVSGPQDNELDG------MQLQPQEYQKMKRGIVEQCCHSTCSLFQLESYCN 106
Zebrafish  -LLG-FLPPKSAQETEVADFAFKDHAELIRKRGIVEQCCHKPCSIFELQNYCN 108
             :      :         :          *****:*** . **:::*:.***

Accession numbers of the insulin sequences used in the protein sequence alignment:  NP_000198 - Human; NP_001008996 - Chimpanzee; NP_032412 - Mouse; NP_001075804 - Rabbit; NP_001079351 - Frog; NP_571131 - Zebrafish

Predicted General Properties of the Insulin Protein  (Homo sapiens) - Primary Sequence Analysis (with ProtParam)b

Number of amino acids: 110 

Molecular weight: 11980.9 

Theoretical pI: 5.22

Total number of negatively charged residues (Asp + Glu): 10

Total number of positively charged residues (Arg + Lys): 7

Atomic composition: Carbon C 535 Hydrogen H 841 Nitrogen N 143 Oxygen O 153 Sulfur S 8

Formula: C535H841N143O153S8

Total number of atoms: 1680

Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 17335 Abs 0.1% (=1 g/l) 1.447, assuming ALL Cys residues appear as half cystines Ext. coefficient 16960 Abs 0.1% (=1 g/l) 1.416, assuming NO Cys residues appear as half cystines

Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo).

Instability index: The instability index (II) is computed to be 40.33 This classifies the protein as unstable.

Aliphatic index: 102.91

Grand average of hydropathicity (GRAVY): 0.193

Bioinformatics References

a Higgins D., Thompson J., Gibson T. Thompson J. D., Higgins D. G., Gibson T. J.(1994). CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting,position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 22:4673-4680.

b Gasteiger E., Hoogland C., Gattiker A., Duvaud S., Wilkins M.R., Appel R.D., Bairoch A.; Protein Identification and Analysis Tools on the ExPASy Server;
(In) John M. Walker (ed): The Proteomics Protocols Handbook, Humana Press (2005). pp. 571-607  Full text - Copyright Humana Press.

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