Sequence Alignment of the Hemoglobin Protein (Homo sapiens) (with Clustalw)a
Chain Alignment
of Hemoglobin
alpha -MVLSPADKTNVKAAWGKVGAHAGEYGAEALERMFLSFPTTKTYFPHF-DLSH-----GS 53
beta MVHLTPEEKSAVTALWGKVN--VDEVGGEALGRLLVVYPWTQRFFESFGDLSTPDAVMGN 58
gamma-1 MGHFTEEDKATITSLWGKVN--VEDAGGETLGRLLVVYPWTQRFFDSFGNLSSASAIMGN 58
gamma-2 MGHFTEEDKATITSLWGKVN--VEDAGGETLGRLLVVYPWTQRFFDSFGNLSSASAIMGN 58
delta MVHLTPEEKTAVNALWGKVN--VDAVGGEALGRLLVVYPWTQRFFESFGDLSSPDAVMGN 58
epsilon MVHFTAEEKAAVTSLWSKMN--VEEAGGEALGRLLVVYPWTQRFFDSFGNLSSPSAILGN 58
zeta -MSLTKTERTIIVSMWAKISTQADTIGTETLERLFLSHPQTKTYFPHF-DLHP-----GS 53
:: ::: : : *.*:. . * *:* *::: .* *: :* * :* *.
alpha AQVKGHGKKVADALTNAVAHVDDMPNALSALSDLHAHKLRVDPVNFKLLSHCLLVTLAAH 113
beta PKVKAHGKKVLGAFSDGLAHLDNLKGTFATLSELHCDKLHVDPENFRLLGNVLVCVLAHH 118
gamma-1 PKVKAHGKKVLTSLGDAIKHLDDLKGTFAQLSELHCDKLHVDPENFKLLGNVLVTVLAIH 118
gamma-2 PKVKAHGKKVLTSLGDAIKHLDDLKGTFAQLSELHCDKLHVDPENFKLLGNVLVTVLAIH 118
delta PKVKAHGKKVLGAFSDGLAHLDNLKGTFSQLSELHCDKLHVDPENFRLLGNVLVCVLARN 118
epsilon PKVKAHGKKVLTSFGDAIKNMDNLKPAFAKLSELHCDKLHVDPENFKLLGNVMVIILATH 118
zeta AQLRAHGSKVVAAVGDAVKSIDDIGGALSKLSELHAYILRVDPVNFKLLSHCLLVTLAAR 113
.:::.**.** :. :.: :*:: ::: **:**. *:*** **:**.: :: ** .
alpha LPAEFTPAVHASLDKFLASVSTVLTSKYR 142
beta FGKEFTPPVQAAYQKVVAGVANALAHKYH 147
gamma-1 FGKEFTPEVQASWQKMVTAVASALSSRYH 147
gamma-2 FGKEFTPEVQASWQKMVTGVASALSSRYH 147
delta FGKEFTPQMQAAYQKVVAGVANALAHKYH 147
epsilon FGKEFTPEVQAAWQKLVSAVAIALAHKYH 147
zeta FPADFTAEAHAAWDKFLSVVSSVLTEKYR 142
: :**. :*: :*.:: *: .*: :*:
Accession numbers of the hemoglobin sequences used in the protein sequence alignments: alpha - P69905; beta - P68871; gamma-1 - P69891; gamma-2 - P69892; delta - P02042; epsilon - P02100; zeta - P02008
Predicted General Properties of Hemoglobin, alpha chain (Homo sapiens) - Primary Sequence Analysis (with ProtParam)b
Number of amino acids: 142
Molecular weight: 15257.5
Theoretical pI: 8.72
Total number of negatively charged residues (Asp + Glu): 12
Total number of positively charged residues (Arg + Lys): 14
Atomic composition: Carbon C 690 Hydrogen H 1080 Nitrogen N 188 Oxygen O 195 Sulfur S 4
Formula: C690H1080N188O195S4
Total number of atoms: 2157
Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 9970 Abs 0.1% (=1 g/l) 0.653, assuming ALL Cys residues appear as half cystines Ext. coefficient 9970 Abs 0.1% (=1 g/l) 0.653, assuming NO Cys residues appear as half cystines
Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo).
Instability index: The instability index (II) is computed to be 6.97 This classifies the protein as stable.
Aliphatic index: 90.77
Grand average of hydropathicity (GRAVY): 0.048
Bioinformatics References
a Higgins D., Thompson J., Gibson T. Thompson J. D., Higgins D. G., Gibson T. J.(1994). CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting,position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 22:4673-4680.
b Gasteiger E., Hoogland C., Gattiker A., Duvaud S., Wilkins M.R., Appel R.D., Bairoch A.; Protein Identification and Analysis Tools on the ExPASy Server;
(In) John M. Walker (ed): The Proteomics Protocols Handbook, Humana Press (2005). pp. 571-607 Full text - Copyright Humana Press.
