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The Extracellular Signal-Regulated Kinases (ERK)

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Sequence Alignment of the Extracellular Signal-Regulated Kinases (Homo sapiens)  (with Clustalw)a

Isoform Alignment of ERK

ERK-1   ----MAAAAAQGGGGGEPRRTEGVGPG----VPGEVEMVKGQ----PFDVGPRYTQLQYI 48
ERK-2   ----MAAAAAAG-----------AGP----------EMVRGQ----VFDVGPRYTNLSYI 31
ERK-3   ----MAEK------------------------------FESLMNIHGFDLGSRYMDLKPL 26
ERK-4   ----MAEK------------------------------GDCIASVYGYDLGGRFVDFQPL 26
ERK-5   MAEPLKEEDGEDGSAEPPAREGRTRPHRCLCSAKNLALLKARSFDVTFDVGDEYEIIETI 60
ERK-6   ----MSSPPPARSG-----------------------FYRQEVTKTAWEVRAVYRDLQPV 33
            :                                          :::   :  :. :

ERK-1   GEGAYGMVSSAYDHVRKTRVAIKKIS-PFEHQTYCQRTLREIQILLRFRHENVIGIRDIL 107
ERK-2   GEGAYGMVCSAYDNVNKVRVAIKKIS-PFEHQTYCQRTLREIKILLRFRHENIIGINDII 90
ERK-3   GCGGNGLVFSAVDNDCDKRVAIKKIV--LTDPQSVKHALREIKIIRRLDHDNIVKVFEIL 84
ERK-4   GFGVNGLVLSAVDSRACRKVAVKKIA--LSDARSMKHALREIKIIRRLDHDNIVKVYEVL 84
ERK-5   GNGAYGVVSSARRRLTGQQVAIKKIPNAFDVVTNAKRTLRELKILKHFKHDNIIAIKDIL 120
ERK-6   GSGAYGAVCSAVDGRTGAKVAIKKLYRPFQSELFAKRAYRELRLLKHMRHENVIGLLDVF 93
        * *  * * **       :**:**:   :      ::: **:::: :: *:*:: : :::

ERK-1   RAST---------LEAMRDVYIVQDLMETDLYKLLKS-QQLSNDHICYFLYQILRGLKYI 157
ERK-2   RAPT---------IEQMKDVYIVQDLMETDLYKLLKT-QHLSNDHICYFLYQILRGLKYI 140
ERK-3   GPSGSQLTDDVGSLTELNSVYIVQEYMETDLANVLEQ-GPLLEEHARLFMYQLLRGLKYI 143
ERK-4   GPKG---TDLQGELFKFSVAYIVQEYMETDLARLLEQ-GTLAEEHAKLFMYQLLRGLKYI 140
ERK-5   RPTVP--------YGEFKSVYVVLDLMESDLHQIIHSSQPLTLEHVRYFLYQLLRGLKYM 172
ERK-6   TPDET--------LDDFTDFYLVMPFMGTDLGKLMKH-EKLGEDRIQFLVYQMLKGLRYI 144
         .              :   *:*   * :** .::.    *  ::   ::**:*:**:*:

ERK-1   HSANVLHRDLKPSNLLIN-TTCDLKICDFGLAR-IADPEHDHTGFLTEYVATRWYRAPEI 215
ERK-2   HSANVLHRDLKPSNLLLN-TTCDLKICDFGLAR-VADPDHDHTGFLTEYVATRWYRAPEI 198
ERK-3   HSANVLHRDLKPANLFINTEDLVLKIGDFGLAR-IMDPHYSHKGHLSEGLVTKWYRSPRL 202
ERK-4   HSANVLHRDLKPANIFISTEDLVLKIGDFGLAR-IVDQHYSHKGYLSEGLVTKWYRSPRL 199
ERK-5   HSAQVIHRDLKPSNLLVN-ENCELKIGDFGMARGLCTSPAEHQYFMTEYVATRWYRAPEL 231
ERK-6   HAAGIIHRDLKPGNLAVN-EDCELKILDFGLAR-------QADSEMTGYVVTRWYRAPEV 196
        *:* ::******.*: :.     *** ***:**       .    ::  :.*:***:*.:

ERK-1   MLNSKGYTKSIDIWSVGCILAEMLSNRPIFPGKHYLDQLNHILGILGSPSQEDLNCIINM 275
ERK-2   MLNSKGYTKSIDIWSVGCILAEMLSNRPIFPGKHYLDQLNHILGILGSPSQEDLNCIINL 258
ERK-3   LLSPNNYTKAIDMWAAGCIFAEMLTGKTLFAGAHELEQMQLILESIPVVHEEDRQELLS- 261
ERK-4   LLSPNNYTKAIDMWAAGCILAEMLTGRMLFAGAHELEQMQLILETIPVIREEDKDELLR- 258
ERK-5   MLSLHEYTQAIDLWSVGCIFGEMLARRQLFPGKNYVHQLQLIMMVLGTPSPAVIQAVGAE 291
ERK-6   ILNWMRYTQTVDIWSVGCIMAEMITGKTLFKGSDHLDQLKEIMKVTGTPPAEFVQRLQSD 256
        :*.   **:::*:*:.***:.**:: : :* * . :.*:: *:           : :   

ERK-1   KARNYLQSLPSKTKVAWAKLFPKSDSKALDLLDRMLTFNPNKRITVEEALAHPYLEQYYD 335
ERK-2   KARNYLLSLPHKNKVPWNRLFPNADSKALDLLDKMLTFNPHKRIEVEQALAHPYLEQYYD 318
ERK-3   VIPVYIRNDMTEPHKPLTQLLPGISREALDFLEQILTFSPMDRLTAEEALSHPYMSIYSF 321
ERK-4   VMPSFVS-STWEVKRPLRKLLPEVNSEAIDFLEKILTFNPMDRLTAEMGLQHPYMSPYSC 317
ERK-5   RVRAYIQSLPPRQPVPWETVYPGADRQALSLLGRMLRFEPSARISAAAALRHPFLAKYHD 351
ERK-6   EAKNYMKGLPELEKKDFASILTNASPLAVNLLEKMLVLDAEQRVTAGEALAHPYFESLHD 316
            ::             : .  .  *:.:* ::* :..  *: .  .* **::     

ERK-1   PTDEPVAEEPFTFAMELDDLPKERLKELIFQETA-------------RFQPGVLEAP--- 379
ERK-2   PSDEPIAEAPFKFDMELDDLPKEKLKELIFEETA-------------RFQPGYRS----- 360
ERK-3   PMDEPISSHPFHIEDEVDDILLMDETHSHIYNWE-------------RYHDCQFSEHDWP 368
ERK-4   PEDEPTSQHPFRIEDEIDDIVLMAANQSQLSNWDTCSS---------RYPVSLSSDLEWR 368
ERK-5   PDDEPDCAPPFDFAFDREALTRERIKEAIVAEIEDFHARREGIRQQIRFQPSLQPVASEP 411
ERK-6   TEDEPQVQK-YDDSFDDVDRTLDEWKRVTYKEVLS-------------FKPPRQLGARVS 362
        . ***     :    :         ..    :                :           

ERK-1   ------------------------------------------------------------
ERK-2   ------------------------------------------------------------
ERK-3   VHNNFDIDEVQLDPRALSDVTDEEEVQVDPRKYLDGDREKYLEDPAFDTNYSTEPCWQYS 428
ERK-4   PDRCQDASEVQRDPRGFGALA--EDVQVDPRK---------------DSHSSSERFLEQS 411
ERK-5   GCPDVEMPSPWAPSGDCAMESPPPAPPPCPGPAPDTIDLTLQPPPPVSEPAPPKKDGAIS 471
ERK-6   KETPL------------------------------------------------------- 367
                                                                    

ERK-1   ------------------------------------------------------------
ERK-2   ------------------------------------------------------------
ERK-3   DHHENKYCDLECSHTCNYKTRSSSYLDNLVWRESEVNHYYEPKLIIDLSNWKEQSKEKSD 488
ERK-4   HSSMERAFEADYGRSCDYKVGSPSYLDKLLWRDNKPHHYSEPKLILDLSHWKQAAGAPPT 471
ERK-5   DNTKAALKAALLKSLRSRLRDGPSAPLEAPEPRKPVTAQERQREREEKRRRRQERAKERE 531
ERK-6   ------------------------------------------------------------
                                                                    

ERK-1   ------------------------------------------------------------
ERK-2   ------------------------------------------------------------
ERK-3   KKGKSKCERNGLVKAQIALEEASQQLAGKEREKNQGFDFDSFIAGTIQLSSQHEPTDVVD 548
ERK-4   ATGLADTGAREDEPASLFLEIAQWVKSTQGAQSTPARPPTTPSAACLPRPPPPGP----- 526
ERK-5   KRRQERERKERGAGASGGPSTDPLAGLVLSDNDRSLLERWTRMARPAAPALTSVPAPAPA 591
ERK-6   ------------------------------------------------------------
                                                                    

ERK-1   ------------------------------------------------------------
ERK-2   ------------------------------------------------------------
ERK-3   KLNDLNSSVSQLELKSLISKSVSQEKQEKGMANLAQLEALYQSSWDSQFVSGGEDCFFIN 608
ERK-4   ------------------GGRRRQPPVRPGRVHLPRPEALHQARG-----PAGQ------ 557
ERK-5   PTPTPTPVQPTSPPPGPLAQPTGPQPQSAGSTSGPVPQPACPPPGPAPHPTGPPGPIPVP 651
ERK-6   ------------------------------------------------------------
                                                                    

ERK-1   ------------------------------------------------------------
ERK-2   ------------------------------------------------------------
ERK-3   QFCEVRKDEQVEKENTYTSYLDKFFSRKEDTEMLETEPVEDGKLGERGHEEGFLNNSGEF 668
ERK-4   ------------------------------------------------------------
ERK-5   APPQIATSTSLLAAQSLVPPPGLPGSSTPGVLPYFPPGLPPPDAGGAPQSSMSESPDVNL 711
ERK-6   ------------------------------------------------------------
                                                                    

ERK-1   ------------------------------------------------------------
ERK-2   ------------------------------------------------------------
ERK-3   LFNKQLESIGIPQFHSPVG-------------SPLKSIQATLTPSAMKSSPQIPHQTYSS 715
ERK-4   ------------------------------------------------------------
ERK-5   VTQQLSKSQVEDPLPPVFSGTPKGSGAGYGVGFDLEEFLNQSFDMGVADGPQDGQADSAS 771
ERK-6   ------------------------------------------------------------
                                                                    

ERK-1   --------------------------------------------
ERK-2   --------------------------------------------
ERK-3   ILKHLN-------------------------------------- 721
ERK-4   --------------------------------------------
ERK-5   LSASLLADWLEGHGMNPADIESLQREIQMDSPMLLADLPDLQDP 815
ERK-6   --------------------------------------------

Accession numbers of the extracellular signal-regulated kinases used in the protein sequence alignment:  P27361 - ERK-1; P28482 - ERK-2; Q16659 - ERK-3; P31152 - ERK-4; Q13164 - ERK-5; P53778 - ERK-6

Predicted General Properties of ERK-1 (Homo sapiens) - Primary Sequence Analysis (with ProtParam)b

Number of amino acids: 379 

Molecular weight: 43135.5 

Theoretical pI: 6.28

Total number of negatively charged residues (Asp + Glu): 48

Total number of positively charged residues (Arg + Lys): 44

Atomic composition: Carbon C 1935 Hydrogen H 3042 Nitrogen N 524 Oxygen O 561 Sulfur S 16

Formula: C1935H3042N524O561S16

Total number of atoms: 6078

Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 43695 Abs 0.1% (=1 g/l) 1.013, assuming ALL Cys residues appear as half cystines Ext. coefficient 43320 Abs 0.1% (=1 g/l) 1.004, assuming NO Cys residues appear as half cystines

Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo).

Instability index: The instability index (II) is computed to be 43.06 This classifies the protein as unstable.

Aliphatic index: 91.64

Grand average of hydropathicity (GRAVY): -0.314

Bioinformatics References

a Higgins D., Thompson J., Gibson T. Thompson J. D., Higgins D. G., Gibson T. J.(1994). CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting,position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 22:4673-4680.

b Gasteiger E., Hoogland C., Gattiker A., Duvaud S., Wilkins M.R., Appel R.D., Bairoch A.; Protein Identification and Analysis Tools on the ExPASy Server;
(In) John M. Walker (ed): The Proteomics Protocols Handbook, Humana Press (2005). pp. 571-607  Full text - Copyright Humana Press.

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