Sequence Alignment of the Extracellular Signal-Regulated Kinases (Homo sapiens) (with Clustalw)a
Isoform Alignment
of ERK
ERK-1 ----MAAAAAQGGGGGEPRRTEGVGPG----VPGEVEMVKGQ----PFDVGPRYTQLQYI 48
ERK-2 ----MAAAAAAG-----------AGP----------EMVRGQ----VFDVGPRYTNLSYI 31
ERK-3 ----MAEK------------------------------FESLMNIHGFDLGSRYMDLKPL 26
ERK-4 ----MAEK------------------------------GDCIASVYGYDLGGRFVDFQPL 26
ERK-5 MAEPLKEEDGEDGSAEPPAREGRTRPHRCLCSAKNLALLKARSFDVTFDVGDEYEIIETI 60
ERK-6 ----MSSPPPARSG-----------------------FYRQEVTKTAWEVRAVYRDLQPV 33
: ::: : :. :
ERK-1 GEGAYGMVSSAYDHVRKTRVAIKKIS-PFEHQTYCQRTLREIQILLRFRHENVIGIRDIL 107
ERK-2 GEGAYGMVCSAYDNVNKVRVAIKKIS-PFEHQTYCQRTLREIKILLRFRHENIIGINDII 90
ERK-3 GCGGNGLVFSAVDNDCDKRVAIKKIV--LTDPQSVKHALREIKIIRRLDHDNIVKVFEIL 84
ERK-4 GFGVNGLVLSAVDSRACRKVAVKKIA--LSDARSMKHALREIKIIRRLDHDNIVKVYEVL 84
ERK-5 GNGAYGVVSSARRRLTGQQVAIKKIPNAFDVVTNAKRTLRELKILKHFKHDNIIAIKDIL 120
ERK-6 GSGAYGAVCSAVDGRTGAKVAIKKLYRPFQSELFAKRAYRELRLLKHMRHENVIGLLDVF 93
* * * * ** :**:**: : ::: **:::: :: *:*:: : :::
ERK-1 RAST---------LEAMRDVYIVQDLMETDLYKLLKS-QQLSNDHICYFLYQILRGLKYI 157
ERK-2 RAPT---------IEQMKDVYIVQDLMETDLYKLLKT-QHLSNDHICYFLYQILRGLKYI 140
ERK-3 GPSGSQLTDDVGSLTELNSVYIVQEYMETDLANVLEQ-GPLLEEHARLFMYQLLRGLKYI 143
ERK-4 GPKG---TDLQGELFKFSVAYIVQEYMETDLARLLEQ-GTLAEEHAKLFMYQLLRGLKYI 140
ERK-5 RPTVP--------YGEFKSVYVVLDLMESDLHQIIHSSQPLTLEHVRYFLYQLLRGLKYM 172
ERK-6 TPDET--------LDDFTDFYLVMPFMGTDLGKLMKH-EKLGEDRIQFLVYQMLKGLRYI 144
. : *:* * :** .::. * :: ::**:*:**:*:
ERK-1 HSANVLHRDLKPSNLLIN-TTCDLKICDFGLAR-IADPEHDHTGFLTEYVATRWYRAPEI 215
ERK-2 HSANVLHRDLKPSNLLLN-TTCDLKICDFGLAR-VADPDHDHTGFLTEYVATRWYRAPEI 198
ERK-3 HSANVLHRDLKPANLFINTEDLVLKIGDFGLAR-IMDPHYSHKGHLSEGLVTKWYRSPRL 202
ERK-4 HSANVLHRDLKPANIFISTEDLVLKIGDFGLAR-IVDQHYSHKGYLSEGLVTKWYRSPRL 199
ERK-5 HSAQVIHRDLKPSNLLVN-ENCELKIGDFGMARGLCTSPAEHQYFMTEYVATRWYRAPEL 231
ERK-6 HAAGIIHRDLKPGNLAVN-EDCELKILDFGLAR-------QADSEMTGYVVTRWYRAPEV 196
*:* ::******.*: :. *** ***:** . :: :.*:***:*.:
ERK-1 MLNSKGYTKSIDIWSVGCILAEMLSNRPIFPGKHYLDQLNHILGILGSPSQEDLNCIINM 275
ERK-2 MLNSKGYTKSIDIWSVGCILAEMLSNRPIFPGKHYLDQLNHILGILGSPSQEDLNCIINL 258
ERK-3 LLSPNNYTKAIDMWAAGCIFAEMLTGKTLFAGAHELEQMQLILESIPVVHEEDRQELLS- 261
ERK-4 LLSPNNYTKAIDMWAAGCILAEMLTGRMLFAGAHELEQMQLILETIPVIREEDKDELLR- 258
ERK-5 MLSLHEYTQAIDLWSVGCIFGEMLARRQLFPGKNYVHQLQLIMMVLGTPSPAVIQAVGAE 291
ERK-6 ILNWMRYTQTVDIWSVGCIMAEMITGKTLFKGSDHLDQLKEIMKVTGTPPAEFVQRLQSD 256
:*. **:::*:*:.***:.**:: : :* * . :.*:: *: : :
ERK-1 KARNYLQSLPSKTKVAWAKLFPKSDSKALDLLDRMLTFNPNKRITVEEALAHPYLEQYYD 335
ERK-2 KARNYLLSLPHKNKVPWNRLFPNADSKALDLLDKMLTFNPHKRIEVEQALAHPYLEQYYD 318
ERK-3 VIPVYIRNDMTEPHKPLTQLLPGISREALDFLEQILTFSPMDRLTAEEALSHPYMSIYSF 321
ERK-4 VMPSFVS-STWEVKRPLRKLLPEVNSEAIDFLEKILTFNPMDRLTAEMGLQHPYMSPYSC 317
ERK-5 RVRAYIQSLPPRQPVPWETVYPGADRQALSLLGRMLRFEPSARISAAAALRHPFLAKYHD 351
ERK-6 EAKNYMKGLPELEKKDFASILTNASPLAVNLLEKMLVLDAEQRVTAGEALAHPYFESLHD 316
:: : . . *:.:* ::* :.. *: . .* **::
ERK-1 PTDEPVAEEPFTFAMELDDLPKERLKELIFQETA-------------RFQPGVLEAP--- 379
ERK-2 PSDEPIAEAPFKFDMELDDLPKEKLKELIFEETA-------------RFQPGYRS----- 360
ERK-3 PMDEPISSHPFHIEDEVDDILLMDETHSHIYNWE-------------RYHDCQFSEHDWP 368
ERK-4 PEDEPTSQHPFRIEDEIDDIVLMAANQSQLSNWDTCSS---------RYPVSLSSDLEWR 368
ERK-5 PDDEPDCAPPFDFAFDREALTRERIKEAIVAEIEDFHARREGIRQQIRFQPSLQPVASEP 411
ERK-6 TEDEPQVQK-YDDSFDDVDRTLDEWKRVTYKEVLS-------------FKPPRQLGARVS 362
. *** : : .. : :
ERK-1 ------------------------------------------------------------
ERK-2 ------------------------------------------------------------
ERK-3 VHNNFDIDEVQLDPRALSDVTDEEEVQVDPRKYLDGDREKYLEDPAFDTNYSTEPCWQYS 428
ERK-4 PDRCQDASEVQRDPRGFGALA--EDVQVDPRK---------------DSHSSSERFLEQS 411
ERK-5 GCPDVEMPSPWAPSGDCAMESPPPAPPPCPGPAPDTIDLTLQPPPPVSEPAPPKKDGAIS 471
ERK-6 KETPL------------------------------------------------------- 367
ERK-1 ------------------------------------------------------------
ERK-2 ------------------------------------------------------------
ERK-3 DHHENKYCDLECSHTCNYKTRSSSYLDNLVWRESEVNHYYEPKLIIDLSNWKEQSKEKSD 488
ERK-4 HSSMERAFEADYGRSCDYKVGSPSYLDKLLWRDNKPHHYSEPKLILDLSHWKQAAGAPPT 471
ERK-5 DNTKAALKAALLKSLRSRLRDGPSAPLEAPEPRKPVTAQERQREREEKRRRRQERAKERE 531
ERK-6 ------------------------------------------------------------
ERK-1 ------------------------------------------------------------
ERK-2 ------------------------------------------------------------
ERK-3 KKGKSKCERNGLVKAQIALEEASQQLAGKEREKNQGFDFDSFIAGTIQLSSQHEPTDVVD 548
ERK-4 ATGLADTGAREDEPASLFLEIAQWVKSTQGAQSTPARPPTTPSAACLPRPPPPGP----- 526
ERK-5 KRRQERERKERGAGASGGPSTDPLAGLVLSDNDRSLLERWTRMARPAAPALTSVPAPAPA 591
ERK-6 ------------------------------------------------------------
ERK-1 ------------------------------------------------------------
ERK-2 ------------------------------------------------------------
ERK-3 KLNDLNSSVSQLELKSLISKSVSQEKQEKGMANLAQLEALYQSSWDSQFVSGGEDCFFIN 608
ERK-4 ------------------GGRRRQPPVRPGRVHLPRPEALHQARG-----PAGQ------ 557
ERK-5 PTPTPTPVQPTSPPPGPLAQPTGPQPQSAGSTSGPVPQPACPPPGPAPHPTGPPGPIPVP 651
ERK-6 ------------------------------------------------------------
ERK-1 ------------------------------------------------------------
ERK-2 ------------------------------------------------------------
ERK-3 QFCEVRKDEQVEKENTYTSYLDKFFSRKEDTEMLETEPVEDGKLGERGHEEGFLNNSGEF 668
ERK-4 ------------------------------------------------------------
ERK-5 APPQIATSTSLLAAQSLVPPPGLPGSSTPGVLPYFPPGLPPPDAGGAPQSSMSESPDVNL 711
ERK-6 ------------------------------------------------------------
ERK-1 ------------------------------------------------------------
ERK-2 ------------------------------------------------------------
ERK-3 LFNKQLESIGIPQFHSPVG-------------SPLKSIQATLTPSAMKSSPQIPHQTYSS 715
ERK-4 ------------------------------------------------------------
ERK-5 VTQQLSKSQVEDPLPPVFSGTPKGSGAGYGVGFDLEEFLNQSFDMGVADGPQDGQADSAS 771
ERK-6 ------------------------------------------------------------
ERK-1 --------------------------------------------
ERK-2 --------------------------------------------
ERK-3 ILKHLN-------------------------------------- 721
ERK-4 --------------------------------------------
ERK-5 LSASLLADWLEGHGMNPADIESLQREIQMDSPMLLADLPDLQDP 815
ERK-6 --------------------------------------------
Accession numbers of the extracellular signal-regulated kinases used in the protein sequence alignment: P27361 - ERK-1; P28482 - ERK-2; Q16659 - ERK-3; P31152 - ERK-4;
Q13164 - ERK-5; P53778 - ERK-6
Predicted General Properties of ERK-1 (Homo sapiens) - Primary Sequence Analysis (with ProtParam)b
Number of amino acids: 379
Molecular weight: 43135.5
Theoretical pI: 6.28
Total number of negatively charged residues (Asp + Glu): 48
Total number of positively charged residues (Arg + Lys): 44
Atomic composition: Carbon C 1935 Hydrogen H 3042 Nitrogen N 524 Oxygen O 561 Sulfur S 16
Formula: C1935H3042N524O561S16
Total number of atoms: 6078
Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 43695 Abs 0.1% (=1 g/l) 1.013, assuming ALL Cys residues appear as half cystines Ext. coefficient 43320 Abs 0.1% (=1 g/l) 1.004, assuming NO Cys residues appear as half cystines
Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo).
Instability index: The instability index (II) is computed to be 43.06 This classifies the protein as unstable.
Aliphatic index: 91.64
Grand average of hydropathicity (GRAVY): -0.314
Bioinformatics References
a Higgins D., Thompson J., Gibson T. Thompson J. D., Higgins D. G., Gibson T. J.(1994). CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting,position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 22:4673-4680.
b Gasteiger E., Hoogland C., Gattiker A., Duvaud S., Wilkins M.R., Appel R.D., Bairoch A.; Protein Identification and Analysis Tools on the ExPASy Server;
(In) John M. Walker (ed): The Proteomics Protocols Handbook, Humana Press (2005). pp. 571-607 Full text - Copyright Humana Press.