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The Elastin Protein

Sequence Alignment of the Elastin Protein (with Clustalw)^a

Species Sequence Alignment of Elastin

mouse   MAGLTAVVPQPGVLLILLLNLLHPAQPGGVPGAVPG----GLPGGVPGGVYYPGAGIGGL 56
rat     MAGLTAAVPQPGVLLILLLNLLHPAQPGGVPGAVPGGVPGGLPGGVPGGVYYPGAGIGG- 59
cow     MRSLTAAARRPEVLLLLLC-ILQPSQPGGVPGAVPG--------GVPGGVFFPGAGLGGL 51
human   MAGLTAAAPRPGVLLLLLS-ILHPSRPGGVPGAIPG--------GVPGGVFYPGAGLGA- 50
        * .***.. :* ***:**  :*:*::*******:**        ******::****:*. 

mouse   GGGGGALGPGGKPPKPGAGLLGTFGAGPGGLGGAGPGAGLGAFPAGTFPGAGALVPGGAA 116
rat     GLGGGALGPGGKPPKPGAGLLGAFGAGPGGLGGAGPGAGLSYASR---PG-GVLVPGGGA 115
cow     GVGG--LGPGVKPAKPGVG--GLVGPGLGAEGSALPGAFPGGFFG---------AGGGAA 98
human   -LGGGALGPGGKPLKPVPG--GLAGAGLG----AGLGAFPAVTFP------GALVPGGVA 97
          **  **** ** **  *  *  *.* *    *  **  .             . ** *

mouse   GAAAAYKAAAKAGAGLGGVGGVPGGVGVGGVPGGVGVGGVPGGVGVGGVPGGVGGIGGIG 176
rat     GAAAAYKAAAKAGAGLGGIG---------GVPGGVGVGGVPGAVGVGGVPGAVGGIGGIG 166
cow     GAAAAYKAAAKAGA--AGLG--------------------------------VGGIGGVG 124
human   DAAAAYK-AAKAGAG-------------------------------------LGGVPGVG 119
        .****** ******                                      :**: *:*

mouse   GLGVSTGAVVPQVGAGIGAGGKPGKVPGVGLPGVYPGGVLPGTGARFPGVGVLPGVPTGT 236
rat     GLGVSTGAVVPQLGAGVGAGGKPGKVPGVGLPGVYPGGVLPGTGARFPGVGVLPGVPTGT 226
cow     GLGVSTGAVVPQLGAGVGAGVKPGKVPGVGLPGVYPGGVLPGAGARFPGIGVLPGVPTGA 184
human   GLGVSAGAVVPQP----GAGVKPGKVPGVGLPGVYPGGVLP--GARFPGVGVLPGVPTGA 173
        *****:******     *** ********************  ******:*********:

mouse   GVKAKAPGGGG-AFAGIPGVGPFGGQQPGVPLGYPIKAPKLPGGYGLPYTNGKLPY---- 291
rat     GVKAKVPGGGGGAFSGIPGVGPFGGQQPGVPLGYPIKAPKLPGGYGLPYTNGKLPY---- 282
cow     GVKPKAQVGAG-AFAGIPGVGPFGGQQPGLPLGYPIKAPKLPAGYGLPYKTGKLPYGFGP 243
human   GVKPKAPGVGG-AFAGIPGVGPFGGPQPGVPLGYPIKAPKLPGGYGLPYTTGKLPYGYGP 232
        ***.*.   .* **:********** ***:************.******..*****    

mouse   -GVAGAGGKAGYPTGTGVGSQ-AAAAAAK-AAKYGAGGAGVLP--GVGGGGIPGGAGAIP 346
rat     -GVAGAGGKAGYPTGTGVGSQ-AAVAAAK-AAKYGAGGGGVLP--GVGGGGIPGGAGAIP 337
cow     GGVAGSAGKAGYPTGTGVGPQ-AAAAAAKAAAKLGAGGAGVLPGVGVGGPGIPGAPGAIP 302
human   GGVAGAAGKAGYPTGTGVGPQAAAAAAAKAAAKFGAGAAGVLP--GVGGAGVPGVPGAIP 290
         ****:.************.* **.**** *** ***..****  **** *:** .****

mouse   GIGGIAGAGTPAAAAAAKAAAKAAKYGAAGGLV-PGGPGVRLPGAGIP-----------G 394
rat     GIGGITGAGTPAAAAAAKAAAKAAKYGAAGGLV-PGGPGVRVPGAGIPGVGIPGVGGIPG 396
cow     GIGGIAGVGAPDAAAAAAAAAKAAKFGAAGGLPGVGVPGVGVPGVGVPGVGVPGVG-VPG 361
human   GIGGIAGVGTPAAAAAAAAAAKAAKYGAAAGLV-PGGPGFGPGVVGVP-----------G 338
        *****:*.*:* ***** *******:***.**   * **.    .*:*           *

mouse   VGGIPGVGGIPGVGGPGIGGPGI----VGGPGAVSPAAAAKAAAKAAKYGARGGV---GI 447
rat     VGGIPGVGGIPGVGGPGIGGPGI----VGGPGAVSPAAAAKAAAKAAKYGARGGV---GI 449
cow     VG-VPGVG-VPGVGVPGVGVPGVGVPGVGVPGALSPAATAKAAAKAAKFGARGAVGIGGI 419
human   AG-VPGVG-VPGAGIPVVPGAGIPG--AAVPGVVSPEAAAKAAAKAAKYGARPGVGVGGI 394
        .* :**** :**.* * :  .*:    .. **.:** *:*********:*** .*   **

mouse   PTYGVGAGGFPGYGVGAGAGLGGAS-PAAAAAAAKAAKYGAGGAGALGGLVPGAVPGALP 506
rat     PTYGVGAGGFPGYGVGAGAGLGGASQAAAAAAAAKAAKYGAGGAGTLGGLVPGAVPGALP 509
cow     PTFGLGPGGFPGIGDAA---------AAPAAAAAKAAKIGAGGVGALGGVVPG-APGAIP 469
human   PTYGVGAGGFPGFGVGVGG------IPGVAGVPSVGGVPGVGGVPGVG-ISPEAQAAAAA 447
        **:*:*.***** * ..         .. *...: ..  *.**.  :* : *   ..* .

mouse   GAVP----------------AVPGAGGVPGAGTPAAAAAAAAAKAAAKAG---LGPGVGG 547
rat     GAVPGALPGAVPGALPGAVPGVPGTGGVPGAGTPAAAAAAAAAKAAAKAGQYGLGPGVGG 569
cow     --------------------GLPGVGGVPGVGIPAAAAAKAAAK-AAQFG---LGPGVGV 505
human   ------------------------KAAKYGVGTPAAAAAKAAAK-AAQFG---LVPGVGV 479
                                 ..  *.* ****** **** **: *   * **** 

mouse   VPGG---VGVGGIPGGVGVGGVPGGVGPGGVTGIGAGPGGLGGAGSPAAAKSAAKAAAKA 604
rat     VPGG---VGVGGLPGGVGPGGVTG-IGTG--PGTGLVPGDLGGAGTPAAAKSAAKAAAKA 623
cow     APG------VGVVPG---VGVVPG---VGVAPGIGLGPGGVIGAGVPAAAKSAAKAAAKA 553
human   APGVGVAPGVGVAPGVGLAPGVGVAPGVGVAPGVGVAPG-IGPGGVAAAAKSAAKVAAKA 538
        .**      **  **      *      *  .* *  ** :  .* .********.****

mouse   QYRAAAGLGAGVPGFGAGAGVPGFGAGAGVPGFGAGAGVPGFGAGAGVPGFGAG------ 658
rat     QYRAAAGLGAGVPGLGVGAGVPGFGAGAG-----------GFGAGAGVPGFGAG------ 666
cow     QFRAAAGLPAGVPGLGVGAGVPGLGVGAG---------VPGLGVGAGVP--GPG------ 596
human   QLRAAAGLGAGIPGLGVGVGVPGLGVGAG---------VPGLGVGAGVPGFGAGADEGVR 589
        * ****** **:**:*.*.****:*.***           *:*.*****  *.*      

mouse   -------------------------AVPGSLAASKAAKYGAAGGLGGPGGLGGPGGLGGP 693
rat     -------------------------AVPGSLAASKAAKYGAAGGLGGPGGLGGPGGLGGP 701
cow     -------------------------AVPGTLAAAKAAKF-------GPGGVG---ALGGV 621
human   RSLSPELREGDPSSSQHLPSTPSSPRVPGALAAAKAAKY----------GAAVPGVLGGL 639
                                  ***:***:****:          * .    *** 

mouse   GGLGG----------AGVPGRVAGAAPPAAAAAAAKAAAKAAQYGLG---GAGGLGAGGL 740
rat     GGLGGPGGLGGPGGLGGVPGGVAGGAP--AAAAAAKAAAKAAQYGLG---GAGGLGAGGL 756
cow     GDLGG----------AGIPGGVAGVVP--AAAAAAKAAAKAAQFGLG---GVGGLGVGGL 666
human   GALGG----------VGIPGGVVGAGP-AAAAAAAKAAAKAAQFGLVGAAGLGGLGVGGL 688
        * ***           *:** *.*  *  **************:**    * ****.***

mouse   GAGGLGAGGLGAGGLGAGGLGAGGLGAGGLGAGGGVSPAAAAKAAKYGAAGLGGVLGARP 800
rat     GAGGLGAGGLGAGGLGAGGLGAGGVIPGAVGLGG-VSPAAAAKAAKYGAAGLGGVLGARP 815
cow     GA-----------------------VPGAVGLGG-VSPAAAAKAAKFGAAGLGGVLGAG- 701
human   G------------------------VPGVGGLGG-IPPAAAAKAAKYGAAGLGGVLGGAG 723
        *                         .*  * ** :.*********:**********.  

mouse   FPGGGVAARPGFGLSPIYPGGGAGGLGVGGKPPKPYGGALGALGYQGGGCFGKSCGRKRK 860
rat     FPGGGVAARPGFGLSPIYPGGGAGGLGVGGKPPKPYGGALGALGYQGGGCFGKSCGRKRK 875
cow     --------------QPFPIGGGAGGLGVGGKPPKPFGGALGALGFPGGACLGKSCGRKRK 747
human   ---------------QFPLGGVAARPGFGLSP-----------IFPGGACLGKACGRKRK 757
                        :  ** *.  *.* .*            : **.*:**:******

Accession numbers of the elastin sequences used in the protein sequence alignment:  human - AAC98395; mouse - NP_031951; rat - NP_036854; cow - NP_786966

Predicted General Properties of the Elastin Protein  (Homo sapiens) - Primary Sequence Analysis (with ProtParam)^b

Number of amino acids: 757

Molecular weight: 66135.9

Theoretical pI: 10.40

Total number of negatively charged residues (Asp + Glu): 8

Total number of positively charged residues (Arg + Lys): 47

Atomic composition: Carbon C 3012 Hydrogen H 4845 Nitrogen N 841 Oxygen O 825 Sulfur S 3

Formula: C₃₀₁₂H₄₈₄₅N₈₄₁O₈₂₅S₃

Total number of atoms: 9526

Extinction coefficients: This protein does not contain any Trp residues. Experience shows that this could result in more than 10% error in the computed extinction coefficient. Extinction coefficients are in units of M^-1 cm^-1, at 280 nm measured in water. Ext. coefficient 22475 Abs 0.1% (=1 g/l) 0.340, assuming ALL Cys residues appear as half cystines Ext. coefficient 22350 Abs 0.1% (=1 g/l) 0.338, assuming NO Cys residues appear as half cystines

Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo).

Instability index: The instability index (II) is computed to be 29.68 This classifies the protein as stable.

Aliphatic index: 91.55

Grand average of hydropathicity (GRAVY): 0.617

Bioinformatics References

^a Higgins D., Thompson J., Gibson T. Thompson J. D., Higgins D. G., Gibson T. J.(1994). CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting,position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 22:4673-4680.

^b Gasteiger E., Hoogland C., Gattiker A., Duvaud S., Wilkins M.R., Appel R.D., Bairoch A.; Protein Identification and Analysis Tools on the ExPASy Server;
(In) John M. Walker (ed): The Proteomics Protocols Handbook, Humana Press (2005). pp. 571-607  Full text - Copyright Humana Press.