Sequence Alignment of the Cytochrome c Protein (with Clustalw)a
Species Sequence Alignment
of Cytochrome c
human ---------------------------MGDVEKGKKIFIMKCSQCHTVEKGGKHKTGPNL 33
mouse ---------------------------MGDVEKGKKIFVQKCAQCHTVEKGGKHKTGPNL 33
rat ---------------------------MGDVEKGKKIFVQKCAQCHTVEKGGKHKTGPNL 33
chicken ---------------------------MGDIEKGKKIFVQKCSQCHTVEKGGKHKTGPNL 33
fugu --------------------------MSGDIAKGKKAFVQKCAQCHTVEQGGKHKTGPNL 34
fly-proximal -----------------------MGVPAGDVEKGKKLFVQRCAQCHTVEAGGKHKVGPNL 37
fly-distal -------------------------MGSGDAENGKKIFVQKCAQCHTYEVGGKHKVGPNL 35
yeast ---------------------MPAPFEKGSEKKGATLFKTRCLQCHTVEEGGPHKVGPNL 39
yersinia ------------------------MRKIPIIAGVFSLLITSCTFAASLQDDMNILIAN-- 34
hyphomicrobium MGGRAFRLRRAEVSIKHAMSAMLPKTRTKALLTALTLAAAAAAAPALADVEFRHALDDSA 60
. . :
human HGLFGRK------TGQAPGYSYTAANKNK--------GIIWGEDTLMEYLENPKKYIPGT 79
mouse HGLFGRK------TGQAAGFSYTDANKNK--------GITWGEDTLMEYLENPKKYIPGT 79
rat HGLFGRK------TGQAAGFSYTDANKNK--------GITWGEDTLMEYLENPKKYIPGT 79
chicken HGLFGRK------TGQAEGFSYTDANKNK--------GITWGEDTLMEYLENPKKYIPGT 79
fugu WGLFGRK------TGQAEGFSYTDANKSK--------GIIWSEETLMVYLENPKKYIPGT 80
fly-proximal HGLIGRK------TGQAAGFAYTDANKAK--------GITWNEDTLFEYLENPKKYIPGT 83
fly-distal GGVVGRK------CGTAAGYKYTDANIKK--------GVTWTEGNLDEYLKDPKKYIPGT 81
yeast HGIMGRK------SGQAVGYSYTDANKKK--------GVEWSEQTMSDYLENPKKYIPGT 85
yersinia LGIVSSSTDTKVITSSLEKMRNAALDAQK--------AIPPKLEGKAEDSPEIKDYRHGF 86
hyphomicrobium LDLSPIKGEE--ITDAVKSFRKDGVNPYNGNAEALAAGKTIYEDNCQACHLDDGRGGMGP 118
.: . . : : . : *
human KMIFVGIKKKEERAD--LIAYLKKATNE--------------------------- 105
mouse KMIFAGIKKKGERAD--LIAYLKKATNE--------------------------- 105
rat KMIFAGIKKKGERAD--LIAYLKKATNE--------------------------- 105
chicken KMIFAGIKKKSERVD--LIAYLKDATSK--------------------------- 105
fugu KMIFAGIKKKTERAD--LIAYLKSSTS---------------------------- 105
fly-proximal KMIFAGLKKPNERGD--LIAYLKSATK---------------------------- 108
fly-distal KMVFAGLKKAEERAD--LIAFLKSNK----------------------------- 105
yeast KMAFGGLKKPKDRND--LVTYLASATK---------------------------- 110
yersinia DLLIEQIDKTKQWAEEGNIQEVKKSVGEVINIRNTYHSRYR-------------- 127
hyphomicrobium SLIDNVFVNARANTDVGMFEIIHSGASGAMRSFSQRGVTQDQILKVIAYIHSLKK 173
.: : : : . : .
Accession numbers of the cytochrome c sequences used in the protein sequence alignment: human - NP_061820; mouse - CAA25899; rat - AAA21711; chicken - CAA25046; fufu - ABF22472; fly-proximal - NP_477176; fly-distal - NP_47716; yeast - O13393; yersinia -AAD37314; hyphomicrobium - CAC85638
Predicted General Properties of the Cytochrome c Protein (Homo sapiens) - Primary Sequence Analysis (with ProtParam)b
Number of amino acids: 105
Molecular weight: 11748.7
Theoretical pI: 9.59
Total number of negatively charged residues (Asp + Glu): 11
Total number of positively charged residues (Arg + Lys): 20
Atomic composition: Carbon C 526 Hydrogen H 845 Nitrogen N 143 Oxygen O 149 Sulfur S 6
Formula: C526H845N143O149S6
Total number of atoms: 1669
Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 13075 Abs 0.1% (=1 g/l) 1.113, assuming ALL Cys residues appear as half cystines Ext. coefficient 12950 Abs 0.1% (=1 g/l) 1.102, assuming NO Cys residues appear as half cystines
Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo).
Instability index: The instability index (II) is computed to be 11.38 This classifies the protein as stable.
Aliphatic index: 66.00
Grand average of hydropathicity (GRAVY): -0.706
Bioinformatics References
a Higgins D., Thompson J., Gibson T. Thompson J. D., Higgins D. G., Gibson T. J.(1994). CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting,position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 22:4673-4680.
b Gasteiger E., Hoogland C., Gattiker A., Duvaud S., Wilkins M.R., Appel R.D., Bairoch A.; Protein Identification and Analysis Tools on the ExPASy Server;
(In) John M. Walker (ed): The Proteomics Protocols Handbook, Humana Press (2005). pp. 571-607 Full text - Copyright Humana Press.
