Sequence Alignment of the Coagulation Factor VIII Protein (with Clustalw)a
Isoform and Species Sequence Alignment
of Coagulation Factor VIII
Human-a -----MQIELSTCFFLCLLRFC-------FSATRRYYLGAVELSWDYMQSDLG-ELPVDA 47
Human-b ------------------------------------------------------------
Dog -----MQVELYTCCFLCLLPFS-------LSATRKYYLGAVELSWDYMQSDLLSALHADT 48
Pig -----MQLELSTCVFLCLLPLG-------FSAIRRYYLGAVELSWDYRQSELLRELHVDT 48
Mouse -----MQIALFACFFLSLFNFC-------SSAIRRYYLGAVELSWNYIQSDLLSVLHTDS 48
Rat -----MRAARGLCFFLSLCALC-------SCATRKYYLGAVELPWDYVSGGASGAQRSDS 48
Fugu MRTDTLLLPLPLPLLLLVAAFCGGDAQQPPAAVRQYYIAAVEIGWDY--------IHVED 52
Human-a RFPPRVPKSFPFNTSVVYKKTLFVEFTDHLFNIAKPRPPWMGLLGPTIQAEVYDTVVITL 107
Human-b ------------------------------------------------------------
Dog SFSSRVPGSLPLTTSVTYRKTVFVEFTDDLFNIAKPRPPWMGLLGPTIQAEVYDTVVIVL 108
Pig RFPATAPGALPLGPSVLYKKTVFVEFTDQLFSVARPRPPWMGLLGPTIQAEVYDTVVVTL 108
Mouse RFLPRMSTSFPFNTSIMYKKTVFVEYKDQLFNIAKPRPPWMGLLGPTIWTEVHDTVVITL 108
Rat RFPPGTP---APSAHVVHTKTVFVEYMDRFFHTAKPRPLWMGLLGPTIWTEVHDTVVITL 105
Fugu GDPASEQRKKPKDIPQKYIKAVYREYLDATYTVPKPRPAWTGIQGPVIVAQAGETVLVHF 112
Human-a KNMASHPVSLHAVGVSYWKASEGAEYDDQTSQREKEDDKVFPGGSHTYVWQVLKENGPMA 167
Human-b ------------------------------------------------------------
Dog KNMASHPVSLHAVGVSYWKASEGAEYEDQTSQKEKEDDNVIPGESHTYVWQVLKENGPMA 168
Pig KNMASHPVSLHAVGVSFWKSSEGAEYEDHTSQREKEDDKVLPGKSQTYVWQVLKENGPTA 168
Mouse KNMASHPVSLHAVGVSYWKASEGDEYEDQTSQMEKEDDKVFPGESHTYVWQVLKENGPMA 168
Rat KNMASHPVSLHAVGMSFWKASEGAAYDDHSSPAEKDDDKVLPGESHTYAWQVLGESGPMA 165
Fugu KNLASQPYSISPVGISYWKHSEGAGYDDSTAGHEKEDDAVYPGGYYEYVWDISPKDGPTG 172
Human-a SDPLCLTYSYLSHVDLVKDLNSGLIGALLVCREGSLAKEKTQTLHKFILLFAVFDEGKSW 227
Human-b ------------------------------------------------------------
Dog SDPPCLTYSYFSHVDLVKDLNSGLIGALLVCKEGSLAKERTQTLQEFVLLFAVFDEGKSW 228
Pig SDPPCLTYSYLSHVDLVKDLNSGLIGALLVCREGSLTRERTQNLHEFVLLFAVFDEGKSW 228
Mouse SDPPCLTYSYMSHVDLVKDLNSGLIGALLVCKEGSLSKERTQMLYQFVLLFAVFDEGKSW 228
Rat SDPPCLTYAYLSHVDLVRDLNSGLVGALLVCKEGRLSAERTRMPPHFVLLFAVFDEGRSW 225
Fugu SDPECLTYSYSSQVDPVRDVNSGLIGALLICKMSAFTDEGQRRNQAFVLLFVVFDESKSW 232
Human-a HSETKNSLMQDRDAASARAWPKMHTVNGYVNRSLPGLIGCHRKS-VYWHVIGMGTTPEVH 286
Human-b ------------------------------------------------------------
Dog HSETNASLT------QAEAQHELHTINGYVNRSLPGLTVCHKRS-VYWHVIGMGTTPEVH 281
Pig HSARNDSWTRAMDPAPARAQPAMHTVNGYVNRSLPGLIGCHKKS-VYWHVIGMGTSPEVH 287
Mouse HSETNDSYTQSMDSASARDWPKMHTVNGYVNRSLPGLIGCHRKS-VYWHVIGMGTTPEIH 287
Rat HAATR-------DPASTEAQPTVHTVNGYTNRTLPGLTGCRGTS-VYWHVMAVGTTPDIH 277
Fugu YGEVGERKSRD-KFKRSDSRKEFHTINGYINATLPGLKICQGRNPVIWHLIGMGTAPAIH 291
Human-a SIFLEGHTFLVRNHRQASLEISPITFLTAQTLLMDLGQFLLFCHISSHQHDGMEAYVKVD 346
Human-b ------------------------------------------------------------
Dog SIFLEGHTFLVGNHRQASLEISPITFLTAQTFLMDLGQFLLFCHIPSHQHDGMEAYVKVD 341
Pig SIFLEGHTFLVRHHRQASLEISPLTFLTAQTFLMDLGQFLLFCHISSHHHGGMEAHVRVE 347
Mouse SIFLEGHTFFVRNHRQASLEISPITFLTAQTLLIDLGQFLLFCHISSHKHDGMEAYVKVD 347
Rat SILLEGHTFSVRSHRQASLEISPMTLLTAQTWLMDLGRFLLFCHISSHRHGGMEAYVQVD 337
Fugu LIQFQHHTLEVLTHRKVTVEVTPMTFVTAEMKPATVGSFLISCQIHAPRHDGMSAMFLVE 351
Human-a SCPE---EPQLRMKN-NEEAEDYDDDLTDSEMDVVRFDDDNSPSFIQIRSVAKKHPKTWV 402
Human-b ------------------------------------------------------------
Dog SCPE---EPQLRMKN-NED-KDYDDGLYGSDMDVVSFDDDSSSPFIQIRSVAKKHPKTWV 396
Pig SCAE---EPQLRRKA-DEE-EDYDDNLYDSDMDVVRLDGDDVSPFIQIRSVAKKHPKTWV 402
Mouse SCPE---ESQWQKKNNNEEMEDYDDDLY-SEMDMFTLDYD-SSPFIQIRSVAKKYPKTWI 402
Rat NCPE---APQGQGKR-DEDTEDYDD--LDSEMDVFTWDPD-AAPFVQVRSAAKRHPKTWV 390
Fugu KCPDPVVLPGPDMRNAKPSNDDDYDYNYDGEEMFNIINFKPKELKPQVRSSRGANFQVWE 411
Human-a HYIAAEEEDWDYAPLVLAPDDRSYKSQYLNNGPQRIGRKYKKVRFMAYTDETFKTREAIQ 462
Human-b ------------------------------------------------------------
Dog HYIAAEEEDWDYAPSGPTPNDRSHKNLYLNNGPQRIGKKYKKVRFVAYTDETFKTREAIQ 456
Pig HYISAEEEDWDYAPAVPSPSDRSYKSLYLNSGPQRIGRKYKKARFVAYTDVTFKTRKAIP 462
Mouse HYISAEEEDWDYAPSVPTSDNGSYKSQYLSNGPHRIGRKYKKVRFIAYTDETFKTRETIQ 462
Rat HYIAAEEGDWDYAPAAATLEDGSLRNRYLGRGPGRIGSKYKKVRFVAYTDGTFHTREATP 450
Fugu HYIAIEELTWDYTPHLSS-TDSELQSRFFPTSASRLSYKYKKVAFVEYTDKSFTRRKNT- 469
Human-a HESGILGPLLYGEVGDTLLIIFKNQASRPYNIYPHGITDVRPLYSRRLPKGVKHLKDFPI 522
Human-b ------------------------------------------------------------
Dog YESGILGPLLYGEVGDTLLIIFKKQASRPYNIYPHGINYVTPLHTGRLPKGVKHLKDMPI 516
Pig YESGILGPLLYGEVGDTLLIIFKNKASRPYNIYPHGITDVSALHPGRLLKGWKHLKDMPI 522
Mouse HESGLLGPLLYGEVGDTLLIIFKNQASRPYNIYPHGITDVSPLHARRLPRGIKHVKDLPI 522
Rat REAGLLGPLLYGEVGDSLLIVFKNRASRAYNIHPHGIRDVGAVHAGRLPRGVKHVKDLPI 510
Fugu -EKSLMGPLLKGKVGDQIHIMLKNTASRPFNIYPNGLSSIRPMKRSKNAS-EKDLRTMGV 527
Human-a LPGEIFKYKWTVTVEDGPTKSDPRCLTRYYSSFVNMERDLASGLIGPLLICYKESVDQRG 582
Human-b ------------------------------------------------------------
Dog LPGEIFKYKWTVTVEDGPTKSDPRCLTRYYSSFINLERDLASGLIGPLLICYKESVDQRG 576
Pig LPGETFKYKWTVTVEDGPTKSDPRCLTRYYSSSINLEKDLASGLIGPLLICYKESVDQRG 582
Mouse HPGEIFKYKWTVTVEDGPTKSDPRCLTRYYSSFINPERDLASGLIGPLLICYKESVDQRG 582
Rat RPGETFKYRWTLTAEDGPARSDARCVTRYYASAVDPERDLASGLIGPLLICCKESVDQRG 570
Fugu GPNETFGYMWELTANDRPLEEDPQCLTQLYQSTVDPEKDLASGLVGTLLICKNEAIDQRG 587
Human-a NQIMSDKRNVILFSVFDENRSWYLTENIQRFLPNPAGVQLEDPEFQASNIMHSINGYVFD 642
Human-b ------------------------------------------------------------
Dog NQMMSDKRNVILFSVLDENRSWYLTEDMQRFLPNADVVQPHDPEFQLSNIMHSINGYVFD 636
Pig NQMMSDKRNVILFSVFDENQSWYLAENIQRFLPNPDGLQPQDPEFQASNIMHSINGYVFD 642
Mouse NQMMSDKRNVILFSIFDENQSWYITENMQRFLPNAAKTQPQDPGFQASNIMHSINGYVFD 642
Rat NQMMSDERNVILFSVFDENRSWYISENMRRFLPDEAHVQLQDPEFEASNLMHSINGYVFD 630
Fugu RLVGPDKDWSLVFAVFDENNSWYMKENIHNTTQTPTGYNDTDPDVYNSHVIYTVNGIMFR 647
Human-a SLQLSVCLHEVAYWYILSIGAQTDFLSVFFSGYTFKHKMVYEDTLTLFPFSGETVFMSME 702
Human-b ------------------------------------------------------------
Dog NLQLSVCLHEVAYWYILSVGAQTDFLSVFFSGYTFKHKMVYEDTLTLFPFSGETVFMSME 696
Pig SLQLSVCLHEVAYWYILSVGAQTDFLSVFFSGYTFKHKMVYEDTLTLFPFSGETVFMSME 702
Mouse SLELTVCLHEVAYWHILSVGAQTDFLSIFFSGYTFKHKMVYEDTLTLFPFSGETVFMSME 702
Rat GLELRVCLHEVAAWHVLSVGAQTDFLSVFFSGHTFRHRAVYADTLTVFPHSGVTVFMSMD 690
Fugu RRQFVICKTDVAFWHVASVGTPTEFLSVYFTGNLFQHQGLYQSVLTLFPMSGLTVSMEPE 707
Human-a NPGLWILGCHNSDFRNRGMTALLKVSSCDKNTGDYYEDSYEDISAYLLSKNNAIEPRSFS 762
Human-b ------------------------------------------------------------
Dog NPGLWVLGCHNSDFRNRGMTALLKVSSCNRNIDDYYEDTYEDIPTPLLNENNVIKPRSFS 756
Pig NPGLWVLGCHNSDLRNRGMTALLKVYSCDRDIGDYYDNTYEDIPGFLLSGKNVIEPRSFA 762
Mouse NPGLWVLGCHNSDFRKRGMTALLKVSSCDKSTSDYYEEIYEDIPTQLVNENNVIDPRSFF 762
Rat NPGVWVLGCHNPDFRESGMTALLKVSSCDEGVSDYYGETYEGVLAPLAN-DNAVDPRSFS 749
Fugu VVGEWEIGAFDGSLKSRGMSIQYTVLTCERED---------------LLVDHELDEVDFS 752
Human-a QNSRHPSTRQKQFNATTIPENDIEKTDPWFAHRTPMPKIQNVSSSDLLMLLRQ-SPTPHG 821
Human-b ------------------------------------------------------------
Dog QNSRHPSTKEKQLKATTTPENDIEKIDLQSGERTQLIKAQSVSSSDLLMLLGQ-NPTPRG 815
Pig QNSRPPSASQKQFQTITSPEDDVE-LDPQSGERTQALEELSVPSGDGSMLLGQ-NPAPHG 820
Mouse QNTNHPNTRKKKFKDSTIPKNDMEKIEPQFEEIAEMLKVQSVSVSDMLMLLGQSHPTPHG 822
Rat QNSNHLHSRKK--------------------------------KSNTPMRFPLSHPTQHG 777
Fugu DYVDN-------------------------------------------MVLGLRG----- 764
Human-a LSLSDLQEAKYETFSDDPSPGAIDSNNSLSEMTHFRPQLHHSGDMVFTPESGLQLRLNEK 881
Human-b ------------------------------------------------------------
Dog LFLSDLREATDR--ADDHSRGAIERNKGPPEVASLRPELRHSEDREFTPEPELQLRLNEN 873
Pig SSSSDLQEARNE--ADDYLPGARERNTAPSAAARLRPELHHSAERVLTPEPEK------- 871
Mouse LFLSDGQEAIYEAIHDDHSPNAIDSNEGPSKVTQLRPESHHSEKIVFTPQPGLQLRSNKS 882
Rat SFWSDSQGDVHDAVHRAHSPAAAHSNEGPAEAAQLEPKPHPGEKMALPP---SWLRAKRS 834
Fugu ------------------------------------------------------------
Human-a LGTTAATELKKLDFKVSST-SNNLIS--TIPSDNLAAGTDNTSSLGPPSMPVHYDSQLDT 938
Human-b ------------------------------------------------------------
Dog LGTNTTVELKKLDLKISSS-SDSLMTSPTIPSDKLAAATEKTGSLGPPNMSVHFNGHLGT 932
Pig -------ELKKLDSKMSSS-SDLLKTSPTIPSDTLSAETERTHSLGPPHPQVNFRSQLGA 923
Mouse LETTIEVKWKKLGLQVSSL-PSNLMTT-TILSDNLKATFEKTDSSGFPDMPVHSSSKLST 940
Rat LVTTMEAKRKKLDLQVQVSGLPDDRTTAVVAPDDPMAACKKAGSSGFP-------DESSP 887
Fugu ------------------------------------------------------------
Human-a TLFGKKSSPLTESGGPLSLSEENNDSKLLESGLMNSQESSWGKNVSSTESGRLFKGKRAH 998
Human-b ------------------------------------------------------------
Dog IVFGNNSSHLIQSGVPLELSEEDNDSKLLEAPLMNIQESSLRENVLSMESNRLFKEERIR 992
Pig IVLGKNSSHFIGAGVPLGSTEED-------------HESSLGENVSPVESDGIFEKERAH 970
Mouse TAFGKKAYSLVGSHVPLNASEENSDSNILDSTLMYSQESLPRDNILSIENDRLLREKRFH 1000
Rat AALGKKMYPRIRPHGPLSIIEGNRDSNSSDSTLMYRLGSPPGDATSWTENRRGLGEKRSH 947
Fugu ------------------------------------------------------------
Human-a GPALLTKDNALFKVSISLLKTNKTSNNSATNRKTHIDGPSLLIENSPSVWQN-ILESDTE 1057
Human-b ------------------------------------------------------------
Dog GPASLIKDNALFKVNISSVKTNRAPVNLTTNRKTRVAIPTLLIENSTSVWQDIMLERNTE 1052
Pig GPASLTKDDVLFKVNISLVKTNKARVYLKTNRKIHIDDAALLTENRAS------------ 1018
Mouse GIALLTKDNTLFKDNVSLMKTNKTYNHSTTNEKLHTESPTS-IENSTTDLQDAILKVNSE 1059
Rat RVAFLARGNTLLSD---------------VDEKSHAPGPTS-VGNSTAAVQDTILEICSE 991
Fugu ------------------------------------------------------------
Human-a FKKVTPLIHDRMLMDKNATALRLNHMSNKTTSSKNMEMVQQKKEGPIPPDAQNPDMSFFK 1117
Human-b ------------------------------------------------------------
Dog FKEVTSLIHNETFMDRNTTALGLNHVSNKTTLSKNVEMAHQKKEDPVPLRAENPDLSSSK 1112
Pig ----------ATFMDKNTTASGLNHVSN-------------------------------- 1036
Mouse IQEVTALIHDGTLLGKNSTYLRLNHMLNRTTSTKNKDIFHRKDEDPIPQDEENTIMPFSK 1119
Rat ILEVTPPIHDRILSDKSATYLRPHRTPDRITSTERKDIRHEKDGDLVPQDADDTSAPFSE 1051
Fugu ------------------------------------------------------------
Human-a MLFLPESARWIQRTHGKNSLNSGQGPSPKQLVSLGPEKSVEGQNFLSEKNKVVVGKGEFT 1177
Human-b ------------------------------------------------------------
Dog IPFLPD---WIK-THGKNSLSSEQRPSPKQLTSLGSEKSVKDQN-FLSEEKVVVGEDEFT 1167
Pig ---------WIKGPLGKNPLSSERGPSPELLTSSGSGKSVKGQSSGQGRIRVAVEEEELS 1087
Mouse MLFLSESSNWFKKTNGNNSLNSEQEHSPKQLVYLMFKKYVKNQSFLSEKNKVTVEQDGFT 1179
Rat APFLSESTDWLKEANGDNSAKPEQEPSPEQLVYLMYIKCMENQSFSSEKNKMIAGQGGFT 1111
Fugu ------------------------------------------------------------
Human-a KDVGLKEMVFPSSRNLFLTNLDNLHENNTHNQEKKIQEEIEKKETLIQENVVLPQIHTVT 1237
Human-b ------------------------------------------------------------
Dog KDTEL-QEIFPNNKSIFFANLANVQENDTYNQEKKSLEEIERKEKLTQENVALPQAHTMI 1226
Pig KGKE---MMLPNSELTFLTNSADVQGNDTHSQGKKSREEMERREKLVQEKVDLPQVYTAT 1144
Mouse KNIGLKDMAFPHNMSIFLTTLSNVHENGRHNQEKNIQEEIEK-EALIEEKVVLPQVHEAT 1238
Rat KNTGLEDTVFPRKTSVFLTTVAKRQESGRHNQE-NIPQAVTEKEAPIEEKGALPQVHIAT 1170
Fugu --------MRPRNHTVLLKVCNKVPINN------------------STHSVNTSTLNATD 798
Human-a GTKNFMKNLFLLSTRQNVEGSYDGAYAPVLQDFRSLNDSTNRTKKHTAHFSKKGE--EEN 1295
Human-b ------------------------------------------------------------
Dog GTKNFLKNLFLLSTKQNVAGLEEQPYTPILQDTRSLNDSPHSEGIHMANFSKIRE--EAN 1284
Pig GTKNFLRNIFHQSTEPSVEGFDGGSHAPVPQDSRSLNDSAERAETHIAHFSAIRE--EAP 1202
Mouse GSKNFLKDILILGTRQNIS--LYEVHVPVLQNITSINNSTNTVQIHMEHFFKRRKDKETN 1296
Rat GSKNFPRDMFVLGTGQNIN-LHEETYVPVHKNVPWTRNPTDTRQIPMVHFFKKRKEEETN 1229
Fugu GRRCYLKKVTLTP----------------------------------------------- 811
Human-a LEGLGNQTKQIVEKYACTTRISPNTSQQNFVTQRSKRALKQFRLPLEETELEKRIIVDDT 1355
Human-b ------------------------------------------------------------
Dog LEGLGNQTNQMVERFPSTTRMS-SNASQHVITQRGKRSLKQPRLSQGEIKFERKVIANDT 1343
Pig LEAPGNRT---------------GPGPRSAVPRRVKQSLKQIRLPLEEIKPERGVVLNAT 1247
Mouse SEGLVNKTREMVKN---------YPSQKNITTQRSKRALGQFRLS--------------- 1332
Rat SGGLVNKTRETVRN---------YPSQKNSVARRREQASGRIKAS--------------- 1265
Fugu ------------------------PGGEDANITSGER----------------------- 824
Human-a STQWSKNMKHLTPSTLTQIDYNEKEKGAITQSPLSDCLTRSHSIPQANRSPLPIAKVSSF 1415
Human-b ------------------------------------------------------------
Dog STQWSKNMNYLAQGTLTQIEYNEKEKRAITQSPLSDCSMRNHVTIQMNDSALPVAKESAS 1403
Pig STRWS------------------------------------------------------- 1252
Mouse -TQWLKTINCSTQCIIKQIDHSKEMKKFITKSSLSD-SSVIKSTTQTNSSDSHIVKTSAF 1390
Rat -ARWLPDVNRSIQSLLKQIDHRKERKKFIIESRSAD-SSGTKSPTQTNHSPSHVVKMSAF 1323
Fugu ------------------------------------------------------------
Human-a PSIRPIYLTRVLFQDNSSHLPAAS----YRKKDSGVQESSHFLQGAKKNNLSLAILTLEM 1471
Human-b ------------------------------------------------------------
Dog PSVRHTDLTKIPSQHNSSHLPASACNYTFRERTSGVQEGSHFLQEAKRNNLSLAFVTLGI 1463
Pig -------------------------------------ESSPILQGAKRNNLSLPFLTLEM 1275
Mouse P---PIDLKRSPFQNKFSHVQASSYIYDFKTKSSRIQESNNFLKETKINNPSLAILPWNM 1447
Rat P----PRDIRRIESRDSSQVWVSSYAYDFETGSSRIRESSPFLNETETDNPSLAVPPRER 1379
Fugu -------------------------------------IPEDILEELQLDGEVTAAPSGIL 847
Human-a TGDQREVGSLGTSATNSVTYKKVENTVLPKPDLPKTSGKVELLPKVHIYQKDLFPTETSN 1531
Human-b ------------------------------------------------------------
Dog TEGQGKFSSLGKSATNQPMYKKLENTVLLQPGLSETSDKVELLSQVHVDQEDSFPTKTSN 1523
Pig AGGQGKISALGKSAAGPLASGKLEKAVLSSAGLSEASGKAEFLPKVRVHREDLLPQKTSN 1335
Mouse FIDQGKFTSPGKSNTNSVTYKKRENIIFLKPTLPEESGKIELLPQVSIQEEEILPTETSH 1507
Rat FVRRGRFASPEKVNTHSAPCDKPENVVSSEPVSPGEAVTVALPPHVSTQEEEPLPAGSSC 1439
Fugu RVGEQALRRRRQAEDNSTDFDIYS---------GDEGGEERSEIQERPAENRTTANATGI 898
Human-a GSPGHLDLVEGSLLQGTEGAIKWNEANRPGKVPFLRVATESSAKTPSKLLDPLAWDNHYG 1591
Human-b ------------------------------------------------------------
Dog DSPGHLDLMGKIFLQKTQGPVKMNKTNSPGKVPFLKWATESSEKIPSKLLGVLAWDNHYD 1583
Pig VSCAHGDLGQEIFLQKTRGPVNLNKVNRPG-------------RTPSKLLG--------- 1373
Mouse GSPGHLNLMKEVFLQKIQGPTKWNKAKRHG--ESIKGKTESSKNTRSKLLNHHAWDYHYA 1565
Rat ERRGHVDLFLEISLQRTRGPVERSRGRRSG------GDTEGHTENPGKTPSP-------L 1486
Fugu NRTKELEEGNEILLTGNPGNSKRS------------------------------------ 922
Human-a TQIPKEEWKSQEKSPEKTAFKKKDTI-LSLNACESNHAIAAINEGQNKPEIEVTWAKQGR 1650
Human-b ------------------------------------------------------------
Dog TQIPSEEWKSQKKSQTNTAFKRKDTI-LPLGPCENNDSTAAINEGQDKPQREAMWAKQGE 1642
Pig PPMP-KEWESLEKSPKSTALRTKDIISLPLDRHESNHSIAAKNEGQAETQREAAWTKQGG 1432
Mouse AQIPKDMWKSKEKSPEIISIKQEDTI-LSLRPHGNSHSIGA-NEKQNWPQRETTWVKQGQ 1623
Rat PQMPKDQRGSEAGYPKICRWR--DGTVLPPRPHNLS---LGAKKKPNLPRREATRVEQGE 1541
Fugu ------------------------------------------------FTVEPVTSEEMV 934
Human-a TERLCSQNPPVLKRHQREITRTTLQSDQEE-----------------IDYDDTISVEMKK 1693
Human-b ------------------------------------------------------------
Dog PGRLCSQNPPVSKHHQREITVTTLQPEEDK-----------------FEYDDTFSIEMKR 1685
Pig PGRLCAPKPPVLRRHQRDISLPTFQPEEDK-----------------MDYDDIFSTETKG 1475
Mouse TQRTCSQIPPVLKRHQRELS--AFQSEQEA-----------------TDYDDAITIET-I 1663
Rat APSLVSPEPLVLRRRPREASTLLPGGEMREDDGDGVTAEYDDDDDVMAEYDDDAVTVDTP 1601
Fugu QNYIMSEEEKLKPHHQQPGNIAVNELSVEY---------------------DDYNQEENS 973
Human-a EDFDIYDEDENQSPRSFQKKTRHYFIAAVERLWDYGMSSSPHVLRNRAQSG----SVPQF 1749
Human-b ------------------------------------------------------------
Dog EDFDIYGDYEDQGLRSFQKKTRHYFIAAVERLWDYGMSRSPHILRNRAQSG----DVQQF 1741
Pig EDFDIYGEDENQDPRSFQKRTRHYFIAAVEQLWDYGMSESPRALRNRAQNG----EVPRF 1531
Mouse EDFDIYSEDIKQGPRSFQQKTRHYFIAAVERLWDYGMSTS-HVLRNRYQSD----NVPQF 1718
Rat EDFDIYGEEAGQGPRGFQQKTRHYFIAAVEELWDYGVRVSPGALGDRAWSG----DAARF 1657
Fugu TSYVLSTDYMD--LRSGEVRYPHYYIAAEEVTWDYGVKKPYQLIRPREMSRGMRKFLPQY 1031
Human-a KKVVFQEFTDGSFTQPLYRGELNEHLGLLGPYIRAEVEDNIMVTFRNQASRPYSFYSSLI 1809
Human-b ------------------------------------------------------------
Dog KKVVFQEFTDGSFTQPLYRGELNEHLGLLGPYIRAEVEDNIVVTFKNQASRPYSFYSSLI 1801
Pig KKVVFREFADGSFTQPSYRGELNKHLGLLGPYIRAEVEDNIMVTFKNQASRPYSFYSSLI 1591
Mouse KKVVFQEFTDGSFSQPLYRGELNEHLGLLGPYIRAEVEDNIMVTFKNQASRPYSFYSSLI 1778
Rat RKVVFREFTDGSFTQRVHRGELDAHLGLLGPYIRAEVEDNIVVTFRNQASRPYSFYSSLV 1717
Fugu KKVVYRAYKDESFRDPVSQGELEAHLGILGPFIKAEVNDLLTVVFKNNASRPYSFHLHGV 1091
Human-a SYEEDQRQGAEPRKNFVKPNETKTYFWKVQHHMAPTKDEFDCKAWAYFSDVDLEKDVHSG 1869
Human-b ------------------------------------------------------------
Dog SYDEDEGQGAEPRRKFVNPNETKIYFWKVQHHMAPTKDEFDCKAWAYFSDVDLEKDVHSG 1861
Pig SYPDDQEQGAEPRHNFVQPNETRTYFWKVQHHMAPTEDEFDCKAWAYFSDVDLEKDVHSG 1651
Mouse SYKEDQ-RGEEPRRNFVKPNETKIYFWKVQHHMAPTEDEFDCKAWAYFSDVDLERDMHSG 1837
Rat SYPEAD-GGAAPRSNFVRPNETKTYFWRVRPHMAPTDGEFDCKAWAYFSDVDLERDLHSG 1776
Fugu YDRNQGASGVLGKP--LAPGEVQTYNWRITKTQGPMDSEFDCKTGTYYSTVDKERDLNSG 1149
Human-a LIGPLLVCHTNTLNPAHGRQVTVQEFALFFTIFDETKSWYFTENMERNCRAPCNIQMEDP 1929
Human-b ------------------------------------------------------------
Dog LIGPLLICRSNTLNPAHGRQVTVQEFALVFTIFDETKSWYFTENLERNCRAPCNVQKEDP 1921
Pig LIGPLLICRANTLNAAHGRQVTVQEFALFFTIFDETKSWYFTENVERNCRAPCHLQMEDP 1711
Mouse LIGPLLICHANTLNPAHGRQVSVQEFALLFTIFDETKSWYFTENVKRNCKTPCNFQMEDP 1897
Rat LIGPLLVCHASTLHPSHARPLAVQEFALLFAVFDETKSWYCAENLDRKCRPPRGTPAGDP 1836
Fugu LIGPLVICKPGTLSTRLHTQPDVQEFALLFHTFDETKSWYMEENLRRYCAPPCHANTQDP 1209
Human-a TFKENYRFHAINGYIMDTLPGLVMAQDQRIRWYLLSMGSNENIHSIHFSGHVFTVRKKEE 1989
Human-b ------------------------------------------------------------
Dog TLKENFRFHAINGYVKDTLPGLVMAQDQKVRWYLLSMGSNENIHSIHFSGHVFTVRKKEE 1981
Pig TLKENYRFHAINGYVMDTLPGLVMAQNQRIRWYLLSMGSNENIHSIHFSGHVFSVRKKEE 1771
Mouse TLKENYRFHAINGYVMDTLPGLVMAQDQRIRWYLLSMGNNENIQSIHFSGHVFTVRKKEE 1957
Rat AWREEYRFHAVNGYVADALLGLVMAEGRRTRWHLLSMGDPGHAQSVHFSAHSVTVRDGGE 1896
Fugu WYHISNKFAAINGYVAESLPGLLVAQHQRVRWHLLNVGSDGEYHAVHFHGLPFTVHAKKE 1269
Human-a YKMALYNLYPGVFETVEMLPSKAGIWRVECLIGEHLHAGMSTLFLVYSNKCQTPLGMASG 2049
Human-b ------------------------------------------------------------
Dog YKMAVYNLYPGVFETVEMLPSQVGIWRIECLIGEHLQAGMSTLFLVYSKKCQTPLGMASG 2041
Pig YKMAVYNLYPGVFETVEMLPSKVGIWRIECLIGEHLQAGMSTTFLVYSKECQAPLGMASG 1831
Mouse YKMAVYNLYPGVFETLEMIPSRAGIWRVECLIGEHLQAGMSTLFLVYSKQCQIPLGMASG 2017
Rat HRTAVCNLYPGVFTTVEMLPSRAGIWRVESLVGEHLRAGMSALFLVYSTRCQVPLGMASG 1956
Fugu HRMGVYNLFPGVFGTVEMRPPTVGTWLVECTVGESQLAGMRAKLLVYNPQCSRPLGMKSG 1329
Human-a HIRDFQITASGQYGQWAPKLARLHYSGSINAWSTKEPFSWIKVDLLAPMIIHGIKTQGAR 2109
Human-b -----------------------------------------------------MRIQDPG 7
Dog HIRDFQITASGQYGQWAPKLARLHYSGSINAWSTKDPFSWIKVDLLAPMIIHGIMTQGAR 2101
Pig RIRDFQITASGQYGQWAPKLARLHYSGSINAWSTKDPHSWIKVDLLAPMIIHGIMTQGAR 1891
Mouse SIRDFQITASGHYGQWAPNLARLHYSGSINAWSTKEPFSWIKVDLLAPMIVHGIKTQGAR 2077
Rat YIRDSQITASGYHGLWTPQLARLHNAGSVNAWSAKEPFAWIKVDLLAPMILHGIETQGAR 2016
Fugu RIGDSQIKASDYIGNWLPHLARLDQSGYINAWMGYNEKSWIQVDLQAPTLLHRVQTQGVR 1389
: *.
Human-a QKFSSLYISQFIIMYSLDGKKWQTYRGN------STGTLMVFFGNVDSSGIKHNIFNPPI 2163
Human-b K---------------------------------------VFFGNVDSSGIKHNIFNPPI 28
Dog QKFSSLYVSQFIIMYSLDGNKWHSYRGN------STGTLMVFFGNVDSSGIKHNIFNPPI 2155
Pig QKFSSLYISQFIIMYSLDGRNWQSYRGN------STGTLMVFFGNVDASGIKHNIFNPPI 1945
Mouse QKFSSLYISQFIIMYSLDGKKWLSYQGN------STGTLMVFFGNVDSSGIKHNSFNPPI 2131
Rat HRLSSLYVSQFIIMYSLDGKQWLSYRGN------STGSLMVFFGNVDASTVRHNRFDPPI 2070
Fugu SNLRNNYITAFTVSYSLDQETWSTYRGSGSSRSSSSSTAKVFNGNLDNSRVKNNPFVPPF 1449
** **:* * :::* * **:
Human-a IARYIRLHPTHYSIRSTLRMELMGCDLNSCSMPLGMESKAISDAQITASSYFTNMFATWS 2223
Human-b IARYIRLHPTHYSIRSTLRMELMGCDLNSCSMPLGMESKAISDAQITASSYFTNMFATWS 88
Dog IAQYIRLHPTHYSIRSTLRMELLGCDFNSCSMPLGMESKAISDAQITASSYLSSMLATWS 2215
Pig VARYIRLHPTHYSIRSTLRMELMGCDLNSCSMPLGMQNKAISDSQITASSHLSNIFATWS 2005
Mouse IARYIRLHPTHSSIRSTLRMELMGCDLNSCSIPLGMESKVISDTQITASSYFTNMFATWS 2191
Rat VARYIRVHPTHASIRTALRMELLGCDLNSCSAPLGMESGWISDTQVTASSHLSTTFSAWP 2130
Fugu VARYIRIHPLYYNQRPALRMELLGCDLNSCSLPLGLQDRRIPDESFVASSSYWSLLRSWT 1509
:*:***:** : . *.:*****:***:**** ***::. *.* ...*** . : :*.
Human-a PSKARLHLQGRSNAWRPQVNNPKEWLQVDFQKTMKVTGVTTQGVKSLLTSMYVKEFLISS 2283
Human-b PSKARLHLQGRSNAWRPQVNNPKEWLQVDFQKTMKVTGVTTQGVKSLLTSMYVKEFLISS 148
Dog PSQARLHLQGRTNAWRPQANNPKEWLQVDFRKTMKVTGITTQGVKSLLISMYVKEFLISS 2275
Pig PSQARLHLQGRTNAWRPRVSSAEEWLQVDLQKTVKVTGITTQGVKSLLSSMYVKEFLVSS 2065
Mouse PSQARLHLQGRTNAWRPQVNDPKQWLQVDLQKTMKVTGIITQGVKSLFTSMFVKEFLISS 2251
Rat PALARLHLRGGANAWRPQVNDPTQWLQVDLQRTVKVTGVVTQGARSLLTAMFVKKFLVST 2190
Fugu PSLARLHQEGSANAWRPKNNNPHEWLQVDLGKVKRITGVVTQGARSLLTKMMVTEFSVTI 1569
*: **** .* :*****: ... :*****: :. ::**: ***.:**: * *.:* ::
Human-a SQDGHQWTLFFQNG--KVKVFQGNQDSFTPVVNSLDPPLLTRYLRIHPQSWVHQIALRME 2341
Human-b SQDGHQWTLFFQNG--KVKVFQGNQDSFTPVVNSLDPPLLTRYLRIHPQSWVHQIALRME 206
Dog SQDGHNWTLFLQND--KVKVFQGNRDSSTPVRNALEPPLVARYVRLHPQSWAHHIALRLE 2333
Pig SQDGRRWTLFLQDG--HTKVFQGNQDSSTPVVNALDPPLFTRYLRIHPTSWAQHIALRLE 2123
Mouse SQDGHHWTQILYNG--KVKVFQGNQDSSTPMMNSLDPPLLTRYLRIHPQIWEHQIALRLE 2309
Rat SQDGRHWTHVLQDG--KVKVFQGNRDASTPMVNSLHPPRFTRYLRIHPQVWERQIALRLE 2248
Fugu SRDGQAWSSVLEGSSQREKIFQGNNDSDEEALTIFDAPLFGRYIRIHPLGWINDIALRLE 1629
*:**: *: .: .. : *:****.*: . :..* . **:*:** * ..****:*
Human-a VLGCEAQDLY 2351
Human-b VLGCEAQDLY 216
Dog VLGCDTQQPA 2343
Pig VLGCEAQDLY 2133
Mouse ILGCEAQQQY 2319
Rat ILGCEAQQLD 2258
Fugu VLGCDTQQAL 1639
:***::*:
Accession numbers of the coagulation factor VIII sequences used in the protein sequence alignment: NP_000123 - Human-a; NP_063916 - Human-b; NP_032003 - Mouse; NP_899160 - Rat; Dog - NP_001003212; NP_999332 - Pig; NP_001027922 - Fugu
Predicted General Properties of the Coagulation Factor VIII Protein (Homo sapiens) - Primary Sequence Analysis (with ProtParam)b
Number of amino acids: 2351
Molecular weight: 267009.3
Theoretical pI: 6.95
Total number of negatively charged residues (Asp + Glu): 269
Total number of positively charged residues (Arg + Lys): 262
Atomic composition: Carbon C 11900 Hydrogen H 18473 Nitrogen N 3243 Oxygen O 3578 Sulfur S 87
Formula: C11900H18473N3243O3578S87
Total number of atoms: 37281
Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 322835 Abs 0.1% (=1 g/l) 1.209, assuming ALL Cys residues appear as half cystines Ext. coefficient 321210 Abs 0.1% (=1 g/l) 1.203, assuming NO Cys residues appear as half cystines
Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo).
Instability index: The instability index (II) is computed to be 43.90 This classifies the protein as unstable.
Aliphatic index: 75.17
Grand average of hydropathicity (GRAVY): -0.517
Bioinformatics References
a Higgins D., Thompson J., Gibson T. Thompson J. D., Higgins D. G., Gibson T. J.(1994). CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting,position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 22:4673-4680.
b Gasteiger E., Hoogland C., Gattiker A., Duvaud S., Wilkins M.R., Appel R.D., Bairoch A.; Protein Identification and Analysis Tools on the ExPASy Server;
(In) John M. Walker (ed): The Proteomics Protocols Handbook, Humana Press (2005). pp. 571-607 Full text - Copyright Humana Press.
