Sequence Alignment of the Cystic Fibrosis Transmembrane Conductance Regulator Protein (with Clustalw)a
Species Sequence Alignment
of CFTR
Rat MQKSPLEKASFISKLFFSWTTPILRKGYRHHLELSDIYQAPSSDSADHLSEKLEREWDRE 60
Mouse MQKSPLEKASFISKLFFSWTTPILRKGYRHHLELSDIYQAPSADSADHLSEKLEREWDRE 60
Human MQRSPLEKASVVSKLFFSWTRPILRKGYRQRLELSDIYQIPSVDSADNLSEKLEREWDRE 60
Monkey MQRSPLEKASVVSKLFFSWTRPILRKGYRQRLELSDIYQIPSADSADNLSEKLEREWDRE 60
Sheep MQRSPLEKASVVSKLFFSWTRPILKKGYRQRLELSDIYHISSSDSADNLSEKLEREWDRE 60
Cow MQRSPLEKASVVSKVFFSWTRPILKKGYRQRLELSDIYHISSSDSADNLSEKLEREWDRE 60
Fugu MQKSPVEDANFLSKFFFWWTSPLLRKGFKKKLELSDVYKAPSFDLADNLSERLEREWDRE 60
**:**:*.*..:**.** ** *:*:**::::*****:*: .* * **:***:********
Rat QASKKK-PQLIHALRRCFVWRFVFYGVLLYLGEVTKAVQPVLLGRIIASYDPDNTEERSI 119
Mouse QASKKN-PQLIHALRRCFFWRFLFYGILLYLGEVTKAVQPVLLGRIIASYDPENKVERSI 119
Human LASKKN-PKLINALRRCFFWRFMFYGIFLYLGEVTKAVQPLLLGRIIASYDPDNKEERSI 119
Monkey LASKKN-PKLINALRRCFFWRFMFYGILLYLGEVTKAVQPLLLGRIIASYDPDNKEERSI 119
Sheep LASKKN-PKLINALRRCFFWRFMFYGIILYLGEVTKAVQPLLLGRIIASYDPDNKVERSI 119
Cow LASKKN-PKLINALRRCFFWRFMFYGIILYLGEVTKAVQPLLLGRIIASYDPDNKVERSI 119
Fugu IVSAKKRPKLMRALARCFLGPFLFFGILLYLGEASKTVQPQLLGRIIASFDPFHAPERSQ 120
.* *: *:*:.** ***. *:*:*::*****.:*:*** ********:** : ***
Rat AIYLGIGLCLLFIVRTLLLHPAIFGLHHIGMQMRIAMFSLIYKKTLKLSSRVLDKISIGQ 179
Mouse AIYLGIGLCLLFIVRTLLLHPAIFGLHRIGMQMRTAMFSLIYKKTLKLSSRVLDKISIGQ 179
Human AIYLGIGLCLLFIVRTLLLHPAIFGLHHIGMQMRIAMFSLIYKKTLKLSSRVLDKISIGQ 179
Monkey AIYLGIGLCLLFIVRTLLLHPAIFGLHHIGMQMRIAMFSLIYKKTLKLSSRVLDKISIGQ 179
Sheep AIYLGIGLCLLFIVRTLLLHPAIFGLHHIGMQMRIAMFSLIYKKTLKLSSRVLDKISIGQ 179
Cow AIYLGIGLCLLFIVRTLLLHPAIFGLHHIGMQMRIAMFSLIYKKTLKLSSRVLDKISIGR 179
Fugu GYFLALGLCLLFTARFLLLQPAIFGLHHLGMQIRIALFSLIYKKTLKLSSRVLDKISTDQ 180
. :*.:****** .* ***:*******::***:* *:******************** .:
Rat LISLLSNNLNKFDEGLALAHFIWIAPLQVVLLMGLLWDLLQFSAFCGLGLLIVLVIFQAI 239
Mouse LVSLLSNNLNKFDEGLALAHFIWIAPLQVTLLMGLLWDLLQFSAFCGLGLLIILVIFQAI 239
Human LVSLLSNNLNKFDEGLALAHFVWIAPLQVALLMGLIWELLQASAFCGLGFLIVLALFQAG 239
Monkey LVSLLSNNLNKFDEGLALAHFVWIVPLQVALLMGLIWELLQASAFCGLGFLIVLALFQAG 239
Sheep LVSLLSNNLNKFDEGLALAHFVWIAPLQVTLLMGLLWDLLQAFTFCGLAFLVVLALLQAG 239
Cow LVSLLSNNLNKFDEGLALAHFVWIAPLQVTLLMGLLWELLQAFTFCGLAFLIVLALLQAG 239
Fugu LVSLMSAHLNKLDESLGLAHFIWITPLQCILCVGLIWELIEVNGFCALAALTLLGIIQAW 240
*:**:* :***:**.*.****:**.*** * :**:*:*:: **.*. * :* ::**
Rat LGKMMVKYRDKRAAKINERLVITSEVIDNIYSVKAYCWESAMEKIIESLREEELKMTRRS 299
Mouse LGKMMVKYRDQRAAKINERLVITSEIIDNIYSVKAYCWESAMEKMIENLREVELKMTRKA 299
Human LGRMMMKYRDQRAGKISERLVITSEMIENIQSVKAYCWEEAMEKMIENLRQTELKLTRKA 299
Monkey LGRMMMKYRDQRAGKINERLVITSEMIENIQSVKAYCWEEAMEKMIENLRQTELKLTRKA 299
Sheep LGKMMMKYRDQRAGKINERLVITSEMIENIQSVKAYCWEEAMEKIIENLRQTELKLTRKA 299
Cow LGKMMMKYRDQRAGKINERVVITSEMIENIQSVKAYCWEEAMEKIIENLRQTELKLTRKA 299
Fugu LSQKMGPHRVKRAGMINRRLALTSEIVENIHSVKAYGWEDVMETIIKNIRQDEMTLTRKI 300
*.: * :* :**. *..*:.:***:::** ***** **..**.:*:.:*: *:.:**:
Rat AYMRFFTSSAFFFSGFFVVFLSVLPYTVINGIVLRKIFTTISFCIVLRMSVTRQFPTAVQ 359
Mouse AYMRFFTSSAFFFSGFFVVFLSVLPYTVINGIVLRKIFTTISFCIVLRMSVTRQFPTAVQ 359
Human AYVRYFNSSAFFFSGFFVVFLSVLPYALIKGIILRKIFTTISFCIVLRMAVTRQFPWAVQ 359
Monkey AYVRYFNSSAFFFSGFFVVFLSVLPYALIKGIVLRKIFTTISFCIVLRMAVTRQFPWAVQ 359
Sheep AYVRYLNSSAFFFSGFFVVFLSVLPYALLKGIILRKIFTTISFCIVLRMAVTRQFPWAVQ 359
Cow AYVRYLNSSAFFFSGFFVVFLSVLPYALLKGIILRKIFTTISFCIVLRMAVTRQFPWAVQ 359
Fugu GYLRYFYSAAYFFSAILVIVSAIVPHALSKGIILRRIFTTASYCMVLRMTLTRQLPGSIQ 360
.*:*:: *:*:***.::*:. :::*::: :**:**:**** *:*:****::***:* ::*
Rat IWYDSLGMIRKIQDFLQTQEYKVLEYNLMFTGLVMENVTAFWEEGFQELLEKVQLNNDDR 419
Mouse IWYDSFGMIRKIQDFLQKQEYKVLEYNLMTTGIIMENVTAFWEEGFGELLEKVQQSNGDR 419
Human TWYDSLGAINKIQDFLQKQEYKTLEYNLTTTEVVMENVTAFWEEGFGELFEKAKQNNNNR 419
Monkey TWYDSLGAINKIQDFLQKQEYKTLEYNLTTTEVVMENVTAFWEEGFGELFEKAKQNNSNR 419
Sheep TWYDSLGAINKIQDFLQKQEYKTLEYNLTTTDVVMENVTAFWEEGFSKLFEKAKENNNNR 419
Cow TWYDSLGAINKIQDFLQKQEYKTLEYNLTTTDVVMDNVTAFWEEGFSKLFEKAKENNNNR 419
Fugu MWYDTLALVKKIEDFLLKEEYRVLEYNLTTTEVELVNVSASWDEGISELFEKIKQENKAN 420
***::. :.**:*** .:**:.***** * : : **:* *:**: :*:** : .* .
Rat KTSNGENHLSFSHLCLVGNPVLKNINLNIKKGEMLAITGSTGAGKTSLLMLILGELEASE 479
Mouse KHSSDENNVSFSHLCLVGNPVLKNINLNIEKGEMLAITGSTGSGKTSLLMLILGELEASE 479
Human KTSNGDDSLFFSNFSLLGTPVLKDINFKIERGQLLAVAGSTGAGKTSLLMVIMGELEPSE 479
Monkey KTSNDDDSLFFSNFSLLGTPVLKDINFKIERGQLLAVAGSTGAGKTSLLMMIMGELEPSE 479
Sheep KISNCDTSLFFSNL-LLGTPVLKDISFKIERGQLLAVAGSTGAGKTSLLMMIMGELEPSE 478
Cow KISNGDNSLFFSNL-LLGTPVLKDISFKIERGQLLAVAGSTGAGKTSLLLMIMGELEASE 478
Fugu GHMANDPGLFFTNL--YVTPVLKNISLYLEKGKMLAVAGSTGAGKSSLLMMILGELVPTE 478
: : *::: .****:*.: :::*::**::****:**:***::*:*** .:*
Rat GIIKHSGRVSFSSQISWIMPGTIKENIIFGVSYDEYRYKSVVKACQLQEDITKFAEQDNT 539
Mouse GIIKHSGRVSFCSQFSWIMPGTIKENIIFGVSYDEYRYKSVVKACQLQQDITKFAEQDNT 539
Human GKIKHSGRISFCSQFSWIMPGTIKENIIFGVSYDEYRYRSVIKACQLEEDISKFAEKDNI 539
Monkey GKIKHSGRISFCSQFSWIMPGTIKENIIFGVSYDEYRYRSVINACQLEEDISKFAEKDNI 539
Sheep GKIKHSGRISFCSQYSWIMPGTIKDNIIFGVSYDEYRYRSVIKACQLEEDISKFSEKDNI 538
Cow GKIKHSGRISFCSQYSWIMPGTIKDNIIFGVSYDEYRYRSVIKACQLEEDISKFAEKDNV 538
Fugu GKIKHSGRISFSPQNSWIMPGTIRDNILFGLTYDEYRYTSVIKACQLEEDFALLPDKDRT 538
* ******:**..* ********::**:**::****** **::****::*:: :.::*.
Rat VLGEGGVTLSGGQRARISLARAVYKDADLYLLDSPFGYLDVLTEEQIFESCVCKLMASKT 599
Mouse VLGEGGVTLSGGQRARISLARAVYKDADLYLLDSPFGYLDVFTEEQVFESCVCKLMANKT 599
Human VLGEGGITLSGGQRARISLARAVYKDADLYLLDSPFGYLDVLTEKEIFESCVCKLMANKT 599
Monkey VLGEGGITLSGGQRARISLARAVYKDADLYLLDSPFGYLDVLTEKEIFESCVCKLMANKT 599
Sheep VLGEGGITLSGGQRARISLARAVYKDADLYLLDSPFGYLDVLTEKEIFESCVCKLMANKT 598
Cow VLGEGGITLSGGQRARISLARAVYKDADLYLLDSPFGYLDVLTEKEIFESCICKLMANKT 598
Fugu LLMEGGVTLSGGQRARLGLARAVYKDADLYLLDAPFTHLDLVTEREIFEKCVCKLMGSKT 598
:* ***:*********:.***************:** :**:.**.::**.*:****..**
Rat RILVTSKMEQLKKADKILILHEGSSYFYGTFSELQSLRPDFSSKLMGYDTFDQFTEERRS 659
Mouse RILVTSKMEHLRKADKILILHQGSSYFYGTFSELQSLRPDFSSKLMGYDTFDQFTEERRS 659
Human RILVTSKMEHLKKADKILILHEGSSYFYGTFSELQNLQPDFSSKLMGCDSFDQFSAERRN 659
Monkey RILVTSKMEHLKKADKILILHEGSSYFYGTFSELQNLRPDFSSKLMGYDSFDQFSAERRN 659
Sheep RILVTSKMEHLKKADKILILHEGSVYFYGTFSELQNQRPDFSSKLMGCDTFDQFTAERRN 658
Cow RILVTSKMEHLKKADKILILHEGSIYFYGTFSELQNQRPDFSSKLMGCDTFDQFTAERRN 658
Fugu RIVVTSKLEHLKRADKILLLHNGDCYFYGTFLELQAQRPDFSSLLLGLEAYDNINAERRS 658
**:****:*:*::*****:**:*. ****** *** :***** *:* :::*::. ***.
Rat SILTETLRRFSVD-DASTTWNKAK-QSFRQTG---------------EFGEKRKNS-ILS 701
Mouse SILTETLRRFSVD-DSSAPWSKPK-QSFRQTG---------------EVGEKRKNS-ILN 701
Human SILTETLHRFSLEGDAPVSWTETKKQSFKQTG---------------EFGEKRKNS-ILN 703
Monkey SILTETLRRFSLEGDAPVSWTETKKQSFKQTG---------------EFGEKRKNS-ILN 703
Sheep SIITETLRRFSLEGDTSVSWNETKKPSFKQTG---------------EFGEKRKNS-ILN 702
Cow SIITETLRRFSLEGDTSVSWNETKKPSFKQTG---------------EFGEKRKNS-ILS 702
Fugu SVLTETLRRVSVDETAGFRGPESIRQSFRQPPPPVIVSGSQGNPGGDGYPEKRKQSLILS 718
*::****:*.*:: : :. **:*. ****:* **.
Rat SFSSVKKISIVQKT----PLSIE---GESDDLQERRLSLVPDSEHGEAALPRSNMITAGP 754
Mouse SFSSVRKISIVQKT----PLCID---GESDDLQEKRLSLVPDSEQGEAALPRSNMIATGP 754
Human PINSIRKFSIVQKT----PLQMNGIEEDSDEPLERRLSLVPDSEQGEAILPRISVISTGP 759
Monkey PINSIRKFSIVQKT----PLQMNGIEEDSDEPLERRLSLVPDSEQGEVILPRISVISTGP 759
Sheep SINSIRKFSVVQKT----SLQMNGIDGASDEPLERRLSLVPHSEPGEGILPRSNAVNSGP 758
Cow SINSIRKFSVVQKT----SLQMNGIEGAADAPLERRLSLVPHSEPGEGILPRSNAVNSGP 758
Fugu PLAAARKFSFMGNSNAGNAAQATATEDGVHEHSERRFSVVPEDDQVEEVLPRSNAYHHGL 778
.: : :*:*.: :: . . *:*:*:**..: * *** . *
Rat TFP-GRRRQSVLDLMTFTPSSVSSSLQR-TRASIR-KISLAPRISLK-EEDIYSRRLSQD 810
Mouse TFP-GRRRQSVLDLMTFTPNSGSSNLQR-TRTSIR-KISLVPQISLN-EVDVYSRRLSQD 810
Human TLQ-ARRRQSVLNLMTHS-VNQGQNIHRKTTASTR-KVSLAPQANLT-ELDIYSRRLSQE 815
Monkey TLQ-ARRRQSVLNLMTHS-VNQGQSIHRKTAASTR-KVSLAPQANLT-ELDIYSRRLSQE 815
Sheep TFL-GGRRQSVLNLMTCSSVNQGQSIHRKTATSTR-KMSLAPQASLA-EIDIYSRRLSQD 815
Cow TFL-GGRRQSVLNLMTGSSVNQGQSIHRKTATSTR-KMSLAPQASLA-EIDIYSRRLSQD 815
Fugu HHLSGQRRQSVLAFITTS---QGQERRAQIQSSFRKKLSITPQCDLASELDIYARRLSKD 835
. ****** ::* : ... : :* * *:*:.*: .* * *:*:****::
Rat STLNITEEINEEDLKECFFDDMVKIPTVTTWNTYLRYFTLHRGLFAVLIWCVLVFLVEVA 870
Mouse STLNITEEINEEDLKECFLDDVIKIPPVTTWNTYLRYFTLHKGLLLVLIWCVLVFLVEVA 870
Human TGLEISEEINEEDLKECFFDDMESIPAVTTWNTYLRYITVHKSLIFVLIWCLVIFLAEVA 875
Monkey TGLEISEEINEEDLKECLFDDMESIPAVTTWNTYLRYITVHKSLIFVLIWCLVIFLAEVA 875
Sheep TGLEISEEINEEDLRDCFFDDVENIPAVTTWNTYLRYITVHKSLMFVLIWCLVVFLVEVA 875
Cow TGLEISEEINEEDLRDCFFDDVENIPAVTTWNTYLRYITVHKSLMFVLIWCLVVFLVEVA 875
Fugu SVYDISEDVDTEDMEQCFADDRDNTFETTSWSTYLRYVSTNKSLVYVLIFIFVVFVIEVA 895
: :*:*::: **:.:*: ** . .*:*.*****.: ::.*. ***: .::*: ***
Rat ASLFVLWLLKNNPVNGGNN------------GTKIANTSYVVVITSSSFYYIFYIYVGVA 918
Mouse ASLFVLWLLKNNPVNSGNN------------GTKISNSSYVVIITSTSFYYIFYIYVGVA 918
Human ASLVVLWLLGNTPLQDKGN------------STHSRNNSYAVIITSTSSYYVFYIYVGVA 923
Monkey ASLVVLWFLGNTPPQDKGN------------STYSRNNSYAVIITRTSSYYVFYIYVGVA 923
Sheep ASLVVLCLFPKILLQDKGN------------STKNASNSYAVIITSTSSYYIFYIYVGVA 923
Cow ASLVVLCLFPKIFFQDKGN------------STKSANNSYAVIITSTSSYYIFYIYVGVA 923
Fugu GSVIGIFLITDTIWRDGANPSSPNYIDQQHSNTSSPPTHLAVIVTPTSAYYIIYIFVATS 955
.*:. : :: . .. * .* . .*::* :* **::**:*..:
Rat DTLLALSLFRGLPLVHTLITASKILHRKMLHSILHAPMSTFNKLKAGGILNRFSKDIAIL 978
Mouse DTLLALSLFRGLPLVHTLITASKILHRKMLHSILHAPMSTISKLKAGGILNRFSKDIAIL 978
Human DTLLAMGFFRGLPLVHTLITVSKILHHKMLHSVLQAPMSTLNTLKAGGILNRFSKDIAIL 983
Monkey DTLLAMGFFRGLPLVHTLITVSKILHHKMLHSVLQAPMSTLNTLKAGGILNRFSKDIAIL 983
Sheep DTLLALGLFRGLPLVHTLITVSKTLHHKMLQSVLQAPMSTLNTLKTGGILNRFSKDIAVL 983
Cow DTLLALGLFRGLPLVHTLITVSKTLHHKMLQSVLQAPMSTLNTLKTGGILNRFSKDIAVL 983
Fugu ESVLALGFFRGLPLVHTLLTVSKRLHEQMLSAVLRAPMAVLNTMKTGRIMNRFTKDMATI 1015
:::**:.:**********:*.** **.:** ::*:***:.:..:*:* *:***:**:* :
Rat DDFLPLTIFDFIQLLFIVVGAIIVVSALQPYIFLATVPGLAVFILLRAYFLHTSQQLKQL 1038
Mouse DDFLPLTIFDFIQLVFIVIGAIIVVSALQPYIFLATVPGLVVFILLRAYFLHTAQQLKQL 1038
Human DDLLPLTIFDFIQLLLIVIGAIAVVAVLQPYIFVATVPVIVAFIMLRAYFLQTSQQLKQL 1043
Monkey DDLLPLTIFDFIQLLLIVIGAIAVVAVLQPYIFVATVPVIVAFIMLRAYFLQTSQQLKQL 1043
Sheep DDLLPLTIFDFIQLLLIVIGAVVVVSVLQPYIFLATVPVIAAFILLRGYFLHTSQQLKQL 1043
Cow DDLLPLTIFDFVQLLLIVIGAVVVVSVLQPYIFLATVPVIAAFILLRAYFLHTSQQLKQL 1043
Fugu DDMLPLVLFDLIQLTLIVTGAIFTVSIMRPYIFIAAIPLAIIFVILRKYFLRTGQQLKLL 1075
**:***.:**::** :** **: .*: ::****:*::* *::** ***:*.**** *
Rat ESEGRSPIFTHLVTSLKGLWTLRAFRRQTYFETLFHKALNLHTANWFMYLATLRWFQMRI 1098
Mouse ESEGRSPIFTHLVTSLKGLWTLRAFRRQTYFETLFHKALNLHTANWFMYLATLRWFQMRI 1098
Human ESEGRSPIFTHLVTSLKGLWTLRAFGRQPYFETLFHKALNLHTANWFLYLSTLRWFQMRI 1103
Monkey ESEGRSPIFTHLVTSLKGLWTLRAFGRQPYFETLFHKALNLHTANWFLYLSTLRWFQMRI 1103
Sheep ESEGRSPIFTHLVTSLKGLWTLRAFGRQPYFETLFHKALNLHTANWFLYLSTLRWFQMRI 1103
Cow ESEGRSPIFTHLVTSLKGLWTLRALDRQPYFETLFHKALNLHTANWFLYLSTLRWFQMRI 1103
Fugu EAEARSPIFSHLIISLKGLWTIRAFGRQTYFETLFHKALNTHTATWFHYLSTLRWFLFRC 1135
*:*.*****:**: *******:**: **.*********** ***.** **:***** :*
Rat DMIFVLFFIVVTFISILTTGEGEGTTGIILTLAMNIMSTLQWAVNSSIDTDSLMRSVSRV 1158
Mouse DMIFVLFFIVVTFISILTTGEGEGTAGIILTLAMNIMSTLQWAVNSSIDTDSLMRSVSRV 1158
Human EMIFVIFFIAVTFISILTTGEGEGRVGIILTLAMNIMSTLQWAVNSSIDVDSLMRSVSRV 1163
Monkey EMIFVIFFIAVTFISILTTGEGEGTVGIILTLAMNIMSTLQWAVNSSIDVDSLMRSVSRV 1163
Sheep EMIFVIFFIAVTFISILTTGEGEGRVGIILTLAMNIMGTLQWAVNSSIDVDSLMRSVSRV 1163
Cow EMIFVIFFIAVTFISILTTGEGEGRVGIILTLAMNIMGTLQWAVNSSIDVDSLMRSVSRV 1163
Fugu DIIFVLFFTAAAFIAVGTNQDKPGEIGIIVALAMLILGTFQWAVITSITVDGLMRSVDRV 1195
::***:** ..:**:: *. : * ***::*** *:.*:**** :** .*.*****.**
Rat FKFIDIQTEESICTKIMKELHSEDSPNALVIKNEHVKKCDTWPSGGEMVVKDLTVKYVDD 1218
Mouse FKFIDIQTEESMYTQIIKELPREGSSDVLVIKNEHVKKSDIWPSGGEMVVKDLTVKYMDD 1218
Human FKFIDMPTE-GKPTKSTKPYKNGQLSKVMIIENSHVKKDDIWPSGGQMTVKDLTAKYTEG 1222
Monkey FKFIDMPTEEGKPTRSTKPYKNGQLSKVMVIENSHVKKDDIWPSGGQMTVKDLTAKYTEG 1223
Sheep FKFIDMPTEDGKPNNSFRPSKDSQPSKVMIIENQHVKKDDIWPSGGQMTVKDLTAKYIDG 1223
Cow FKFIDMPTEDGKPNNSFRPSKDSQPSKVMIIENQHVKKDDIWPSGGQMTVKDLTAKYTDG 1223
Fugu FKFIDLPSEELLPGK-----PGGKGRPDLIIDNPHAQ--DYWPNRGQMDVQGLAVKYTEA 1248
*****: :* . ::*.* *.: * **. *:* *:.*:.** :
Rat GNAILENISFSISPGQRVGLLGRTGSGKSTLLSAFLRMLNIKGEIQIDGVSWNSMTLQEW 1278
Mouse GNAVLENISFSISPGQRVGLLGRTGSGKSTLLSAFLRMLNIKGDIEIDGVSWNSVTLQEW 1278
Human GNAILENISFSISPGQRVGLLGRTGSGKSTLLSAFLRLLNTEGEIQIDGVSWDSITLQQW 1282
Monkey GNPILENISFSISPGQRVGLLGRTGSGKSTLLSAFLRLLNTEGEIQIDGVSWDSITLQQW 1283
Sheep GNAILENISFSISPGQRVGLLGRTGSGKSTLLLAFLRLLNTKGEIQIDGVSWDSITLQQW 1283
Cow GNAILENISFSISSGQRVGLLGRTGSGKSTLLLAFLRLLNTKGEIQIDGVSWDSITLQQW 1283
Fugu GRAVLSDISFSVEGGLSMGLLGRTGSGKSTLLSALLRLASTDGEISIDGISWNSVPLHKW 1308
*..:*.:****:. * :************** *:**: . .*:*.***:**:*:.*::*
Rat RKAFGVITQKVFIFSGTFRQNLDPNGKWRDEEIWKVADQVGLKSVIEQFPGQLNFTLVDG 1338
Mouse RKAFGVITQKVFIFSGTFRQNLDPNGKWKDEEIWKVADEVGLKSVIEQFPGQLNFTLVDG 1338
Human RKAFGVIPQKVFIFSGTFRKNLDPYEQWSDQEIWKVADEVGLRSVIEQFPGKLDFVLVDG 1342
Monkey RKAFGVIPQKVFIFSGTFRKNLDPYEQWSDQEIWKVADEVGLRSVIEQFPGKLDFVLVDG 1343
Sheep RKAFGVIPQKVFIFSGTFRKNLDPYEQWSDQEIWKVADEVGLRSVIEQFPGKLDFVLVDG 1343
Cow RKAFGVIPQKVFIFSGTFRKNLDPYGQWSDQEIWKVADEVGLRAVIEQFPGKLDFVLVDG 1343
Fugu RKAFGVVPQKVFILTGTFRMNLDPHGRYSDEELWRVADQVGLKSVIEQFPDRLDFQLENG 1368
******:.*****::**** **** :: *:*:*:***:***::******.:*:* * :*
Rat GYVLSHGHKQLMCLARSVLSKAKIILLDEPSANLDPITYQVIRRVLRQAFAGCTVVLCEH 1398
Mouse GYVLSHGHKQLMCLARSVLSKAKIILLDEPSAHLDPITYQVIRRVLKQAFAGCTVILCEH 1398
Human GCVLSHGHKQLMCLARSVLSKAKILLLDEPSAHLDPVTYQIIRRTLKQAFADCTVILCEH 1402
Monkey GCVLSHGHKQLMCLARSVLSKAKILLLDEPSAHLDPVTYQIIRRTLKQAFADCTVILCEH 1403
Sheep GCVLSHGHKQLMCLARSVLSKAKILLLDEPSAHLDPITYQIIRRTLKQAFADCTVILSEH 1403
Cow GCVLSHGHKQLMCLARSVLSKAKILLLDEPSAHLDPITYQIIRRTLKQAFANCTVILSEH 1403
Fugu GSVLSHGHKQLMCLARSILSKARILLLDEPSAYLDTITLQVLRKTLKHAFSDCTVILSEH 1428
* ***************:****:*:******* **.:* *::*:.*::**:.***:*.**
Rat RIEAMLDCQRFLVIEQGNVWQYESLQALLSEKSVFQRALSSSEKMKLFHGRHSSKQKPRT 1458
Mouse RIEAMLDCQRFLVIEESNVWQYDSLQALLSEKSIFQQAISSSEKMRFFQGRHSSKHKPRT 1458
Human RIEAMLECQQFLVIEENKVRQYDSIQKLLNERSLFRQAISPSDRVKLFPHRNSSKCKSKP 1462
Monkey RIEAMLECQQFLVIEENKVRQYDSIQKLLNERSLFRQAISPSDRVKLFPHRNSSKCKTQP 1463
Sheep RIEAMLECQRFLVIEENKVRQYDSIQRMLSEKSLFRQAISPADRLKLLPHRNSSRQRSRA 1463
Cow RIEAMLECQRFFVIEENKVRQYDSIQRMLSEKSLFRQAISPADRLKLLPHRNSSRQRSRS 1463
Fugu KVEPLLECQSFLVIEGSSVKSYDSIQKLLNEMSHLKQAVSAADRLRLFPTLHRLNSIKRA 1488
::*.:*:** *:*** ..* .*:*:* :*.* * :::*:*.::::::: : . :.
Rat -----QITAVKEETEEEVQETRL 1476
Mouse -----QITALKEETEEEVQETRL 1476
Human -----QIAALKEETEEEVQDTRL 1480
Monkey -----QIAALKEETEEEVQDTRL 1481
Sheep -----NIAALKEETEEEVQETKL 1481
Cow -----NIAALKEETEEEVQETKL 1481
Fugu PPQAAKISSLPEEAEDEVHDTRL 1511
:*::: **:*:**::*:*
Accession numbers of the cystic fibrosis transmembrane conductance regulators used in the protein sequence alignment: Human - NP_000483; Mouse - NP_066388; Rat - NP_113694; Monkey - ; Cow -
NP_776443; Sheep - NP_001009781; Fugu - NP_001041505
Predicted General Properties of the Cystic Fibrosis Transmembrane Conductance Regulator Protein (Homo sapiens) - Primary Sequence Analysis (with ProtParam)b
Number of amino acids: 1480
Molecular weight: 168141.5
Theoretical pI: 8.91
Total number of negatively charged residues (Asp + Glu): 151
Total number of positively charged residues (Arg + Lys): 170
Atomic composition: Carbon C 7640 Hydrogen H 12106 Nitrogen N 2000 Oxygen O 2150 Sulfur S 55
Formula: C7640H12106N2000O2150S55
Total number of atoms: 23951
Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 187225 Abs 0.1% (=1 g/l) 1.113, assuming ALL Cys residues appear as half cystines Ext. coefficient 186100 Abs 0.1% (=1 g/l) 1.107, assuming NO Cys residues appear as half cystines
Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo).
Instability index: The instability index (II) is computed to be 43.80 This classifies the protein as unstable.
Aliphatic index: 102.82
Grand average of hydropathicity (GRAVY): 0.024
Bioinformatics References
a Higgins D., Thompson J., Gibson T. Thompson J. D., Higgins D. G., Gibson T. J.(1994). CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting,position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 22:4673-4680.
b Gasteiger E., Hoogland C., Gattiker A., Duvaud S., Wilkins M.R., Appel R.D., Bairoch A.; Protein Identification and Analysis Tools on the ExPASy Server;
(In) John M. Walker (ed): The Proteomics Protocols Handbook, Humana Press (2005). pp. 571-607 Full text - Copyright Humana Press.
