Sequence Alignment of the Adrenergic Receptor (Homo sapiens) (with Clustalw)a
Isoform Sequence Alignment
of the Adrenergic Receptor
alpha1A-1 --------------------------------MVFLSGNASDSSNCTQPPAPVNISKAIL 28
alpha1A-3 --------------------------------MVFLSGNASDSSNCTQPPAPVNISKAIL 28
alpha1A-4 --------------------------------MVFLSGNASDSSNCTQPPAPVNISKAIL 28
alpha1A-2 --------------------------------MVFLSGNASDSSNCTQPPAPVNISKAIL 28
alpha1B -----------MNPDLDTGHNTSAPAHWGELKNANFTGPNQTSSNSTLP--QLDITRAIS 47
beta1 MGAGVLVLGASEPGNLSSAAPLPDGAATAARLLVPASPPASLLPPASESPEPLSQQWTAG 60
beta2 MGQ---------PGNGS------------AFLLAPNRSHA----PDHDVTQQRDEVWVVG 35
beta3 MAP--------WPHENS--------------SLAPWPDLPTLAPNTANTSGLPGVPWEAA 38
alpha2A --------MGS--------LQPDAG-NASWNGTEAPGGGARAT---------PYSLQVTL 34
alpha2C --------MASPALAAALAVAAAAGPNASGAGERGSGGVANASGASWGPPRGQYSAGAVA 52
alpha2B --------MDHQDP---------------------------------------YSVQATA 13
alpha1A-1 LG-VILGGLILFGVLGNILVILSVACHRHLHSVTHYYIVNLAVADLLLTSTVLPFSAIFE 87
alpha1A-3 LG-VILGGLILFGVLGNILVILSVACHRHLHSVTHYYIVNLAVADLLLTSTVLPFSAIFE 87
alpha1A-4 LG-VILGGLILFGVLGNILVILSVACHRHLHSVTHYYIVNLAVADLLLTSTVLPFSAIFE 87
alpha1A-2 LG-VILGGLILFGVLGNILVILSVACHRHLHSVTHYYIVNLAVADLLLTSTVLPFSAIFE 87
alpha1B VG-LVLGAFILFAIVGNILVILSVACNRHLRTPTNYFIVNLAMADLLLSFTVLPFSAALE 106
beta1 MG-LLMALIVLLIVAGNVLVIVAIAKTPRLQTLTNLFIMSLASADLVMGLLVVPFGATIV 119
beta2 MG-IVMSLIVLAIVFGNVLVITAIAKFERLQTVTNYFITSLACADLVMGLAVVPFGAAHI 94
beta3 LAGALLALAVLATVGGNLLVIVAIAWTPRLQTMTNVFVTSLAAADLVMGLLVVPPAATLA 98
alpha2A TLVCLAGLLMLLTVFGNVLVIIAVFTSRALKAPQNLFLVSLASADILVATLVIPFSLANE 94
alpha2C GLAAVVGFLIVFTVVGNVLVVIAVLTSRALRAPQNLFLVSLASADILVATLVMPFSLANE 112
alpha2B AIAAAITFLILFTIFGNALVILAVLTSRSLRAPQNLFLVSLAAADILVATLIIPFSLANE 73
:: : ** **: :: *:: : :: .** **::: ::* .
alpha1A-1 VLGYWAFGRVFCNIWAAVDVLCCTASIMGLCIISIDRYIGVSYPLRYPTIVTQRRGLMAL 147
alpha1A-3 VLGYWAFGRVFCNIWAAVDVLCCTASIMGLCIISIDRYIGVSYPLRYPTIVTQRRGLMAL 147
alpha1A-4 VLGYWAFGRVFCNIWAAVDVLCCTASIMGLCIISIDRYIGVSYPLRYPTIVTQRRGLMAL 147
alpha1A-2 VLGYWAFGRVFCNIWAAVDVLCCTASIMGLCIISIDRYIGVSYPLRYPTIVTQRRGLMAL 147
alpha1B VLGYWVLGRIFCDIWAAVDVLCCTASILSLCAISIDRYIGVRYSLQYPTLVTRRKAILAL 166
beta1 VWGRWEYGSFFCELWTSVDVLCVTASIETLCVIALDRYLAITSPFRYQSLLTRARARGLV 179
beta2 LMKMWTFGNFWCEFWTSIDVLCVTASIETLCVIAVDRYFAITSPFKYQSLLTKNKARVII 154
beta3 LTGHWPLGATGCELWTSVDVLCVTASIETLCALAVDRYLAVTNPLRYGALVTKRCARTAV 158
alpha2A VMGYWYFGKAWCEIYLALDVLFCTSSIVHLCAISLDRYWSITQAIEYNLKRTPRRIKAII 154
alpha2C LMAYWYFGQVWCGVYLALDVLFCTSSIVHLCAISLDRYWSVTQAVEYNLKRTPRRVKATI 172
alpha2B LLGYWYFRRTWCEVYLALDVLFCTSSIVHLCAISLDRYWAVSRALEYNSKRTPRRIKCII 133
: * * .: ::*** *:** ** :::*** .: ...* * :
alpha1A-1 LCVWALSLVISIGPLFGWRQPAPED---------ETICQINEEPGYVLFSALGSFYLPLA 198
alpha1A-3 LCVWALSLVISIGPLFGWRQPAPED---------ETICQINEEPGYVLFSALGSFYLPLA 198
alpha1A-4 LCVWALSLVISIGPLFGWRQPAPED---------ETICQINEEPGYVLFSALGSFYLPLA 198
alpha1A-2 LCVWALSLVISIGPLFGWRQPAPED---------ETICQINEEPGYVLFSALGSFYLPLA 198
alpha1B LSVWVLSTVISIGPLLGWKEPAPND---------DKECGVTEEPFYALFSSLGSFYIPLA 217
beta1 CTVWAISALVSFLPILMHWWRAESD-EARRCYNDPKCCDFVTNRAYAIASSVVSFYVPLC 238
beta2 LMVWIVSGLTSFLPIQMHWYRATHQ-EAINCYANETCCDFFTNQAYAIASSIVSFYVPLV 213
beta3 VLVWVVSAAVSFAPIMSQWWRVGADAEAQRCHSNPRCCAFASNMPYVLLSSSVSFYLPLL 218
alpha2A ITVWVISAVISFPPLISIEKKGGGG----GPQPAEPRCEINDQKWYVISSCIGSFFAPCL 210
alpha2C VAVWLISAVISFPPLVSLYRQPDG--------AAYPQCGLNDETWYILSSCIGSFFAPCL 224
alpha2B LTVWLIAAVISLPPLIYKGDQGPQ-------PRGRPQCKLNQEAWYILASSIGSFFAPCL 186
** :: *: *: * . : * : *. **: *
alpha1A-1 IILVMYCRVYVVAKRESRGLK---------------------------SGLKTDKSDSEQ 231
alpha1A-3 IILVMYCRVYVVAKRESRGLK---------------------------SGLKTDKSDSEQ 231
alpha1A-4 IILVMYCRVYVVAKRESRGLK---------------------------SGLKTDKSDSEQ 231
alpha1A-2 IILVMYCRVYVVAKRESRGLK---------------------------SGLKTDKSDSEQ 231
alpha1B VILVMYCRVYIVAKRTTKNLE---------------------------AGVMKEMSNSKE 250
beta1 IMAFVYLRVFREAQKQVKKID---------------------------SCERRFLGGPAR 271
beta2 IMVFVYSRVFQEAKRQLQKID---------------------------KSEGRFH----- 241
beta3 VMLFVYARVFVVATRQLRLLR---------------------------GELGRFPPEES- 250
alpha2A IMILVYVRIYQIAKRRTRVPPSRRGPDAVAAPPGGTERRPNGLGPERSAGPGGAEAEPLP 270
alpha2C IMGLVYARIYRVAKLRTRTLSEKRAP---VGPDGASPTTENGLGAAAGAGENG-HCAPPP 280
alpha2B IMILVYLRIYLIAKRSNRRGPRAKGG---PGQGESKQPRPDHGGALASAKLPALASVASA 243
:: .:* *:: * :
alpha1A-1 VTLRIHRKNAPAGGSGMASAK-------------TKTHFSVRL----------------- 261
alpha1A-3 VTLRIHRKNAPAGGSGMASAK-------------TKTHFSVRL----------------- 261
alpha1A-4 VTLRIHRKNAPAGGSGMASAK-------------TKTHFSVRL----------------- 261
alpha1A-2 VTLRIHRKNAPAGGSGMASAK-------------TKTHFSVRL----------------- 261
alpha1B LTLRIHSKNFHEDTLSSTKAKG----------HNPRSSIAVKL----------------- 283
beta1 PPSPSPSPVPAPAPPPGPPRPAAAAATAPLANGRAGK-RRPSR----------------- 313
beta2 --VQNLSQVEQ--------------------DGRTGHGLRRSS----------------- 262
beta3 PPAPSRSLAPAPVGTCAPP------------EGVPACGRRPAR----------------- 281
alpha2A TQLNGAPGEPAPAGPRDTDALDLEESSSSDHAERPPGPRRPERGPRGKGKARASQVKPGD 330
alpha2C ADVE--PDESSAAAERRRRRGALRRG-----GRRRAGAEGGAGGADGQG-AGPGAAESGA 332
alpha2B REVNGHSKSTGEKEEGETPEDTGTRALPPSWAALPNSGQGQKEGVCGASPEDEAEEEEEE 303
alpha1A-1 ---------------------------------------------------------LKF 264
alpha1A-3 ---------------------------------------------------------LKF 264
alpha1A-4 ---------------------------------------------------------LKF 264
alpha1A-2 ---------------------------------------------------------LKF 264
alpha1B ---------------------------------------------------------FKF 286
beta1 ---------------------------------------------------------LVA 316
beta2 ---------------------------------------------------------KFC 265
beta3 ---------------------------------------------------------LLP 284
alpha2A SLPRRGPGATGI-GTPAAGP------GEERVGAAKAS-----------------RWRGRQ 366
alpha2C LTASRSPGPGGR-LSRASSRSVEFFLSRRRRARSSVC-----------------RRKVAQ 374
alpha2B EEEEEECEPQAVPVSPASACSPPLQQPQGSRVLATLRGQVLLGRGVGAIGGQWWRRRAQL 363
alpha1A-1 SREKKAAKTLGIVVGCFVLCWLPFFLVMPIGSFFPD-FKPSETVFKIVFWLGYLNSCINP 323
alpha1A-3 SREKKAAKTLGIVVGCFVLCWLPFFLVMPIGSFFPD-FKPSETVFKIVFWLGYLNSCINP 323
alpha1A-4 SREKKAAKTLGIVVGCFVLCWLPFFLVMPIGSFFPD-FKPSETVFKIVFWLGYLNSCINP 323
alpha1A-2 SREKKAAKTLGIVVGCFVLCWLPFFLVMPIGSFFPD-FKPSETVFKIVFWLGYLNSCINP 323
alpha1B SREKKAAKTLGIVVGMFILCWLPFFIALPLGSLFST-LKPPDAVFKVVFWLGYFNSCLNP 345
beta1 LREQKALKTLGIIMGVFTLCWLPFFLANVVKAFHR--ELVPDRLFVFFNWLGYANSAFNP 374
beta2 LKEHKALKTLGIIMGTFTLCWLPFFIVNIVHVIQD--NLIRKEVYILLNWIGYVNSGFNP 323
beta3 LREHRALCTLGLIMGTFTLCWLPFFLANVLRALGGP-SLVPGPAFLALNWLGYANSAFNP 343
alpha2A NREKRFTFVLAVVIGVFVVCWFPFFFTYTLTAVG---CSVPRTLFKFFFWFGYCNSSLNP 423
alpha2C AREKRFTFVLAVVMGVFVLCWFPFFFSYSLYGICREACQVPGPLFKFFFWIGYCNSSLNP 434
alpha2B TREKRFTFVLAVVIGVFVLCWFPFFFSYSLGAICPKHCKVPHGLFQFFFWIGYCNSSLNP 423
:*:: .*.:::* * :**:***: : . : . *:** ** :**
alpha1A-1 IIYPCSSQEFKKAFQNVLRIQCLCR---KQSSKHALG---YTLHP--------PSQAVEG 369
alpha1A-3 IIYPCSSQEFKKAFQNVLRIQCLCR---KQSSKHALG---YTLHP--------PSQAVEG 369
alpha1A-4 IIYPCSSQEFKKAFQNVLRIQCLCR---KQSSKHALG---YTLHP--------PSQAVEG 369
alpha1A-2 IIYPCSSQEFKKAFQNVLRIQCLCR---KQSSKHALG---YTLHP--------PSQAVEG 369
alpha1B IIYPCSSKEFKRAFVRILGCQCRGRGRRRRRRRRRLGGCAYTYRPWTRGGSLERSQSRKD 405
beta1 IIY-CRSPDFRKAFQGLLCCARRAARRRHATHGDRPRA--------------SGCLARPG 419
beta2 LIY-CRSPDFRIAFQELLCLRRSSLK----AYGNGYSS--------------NGNTGEQ- 363
beta3 LIY-CRSPDFRSAFRRLLCR-----------CGRRLPP--------------EPCAAAR- 376
alpha2A VIYTIFNHDFRRAFKKILCRGDRKRIV--------------------------------- 450
alpha2C VIYTVFNQDFRRSFKHILFRRRRRGFRQ-------------------------------- 462
alpha2B VIYTIFNQDFRRAFRRILCRPWTQTAW--------------------------------- 450
:** . :*: :* :*
alpha1A-1 QHKDMVRIPVGSRETFYRISKTDG--------VCEWKFFSSMPRGSARITVSKDQSSCTT 421
alpha1A-3 QHKDMVRIPVGSRETFYRISKTDG--------VCEWKFFSSMPRGSARITVSKDQSSCTT 421
alpha1A-4 QHKDMVRIPVGSRETFYRISKTDG--------VCEWKFFSSMPRGSARITVSKDQSSCTT 421
alpha1A-2 QHKDMVRIPVGSRETFYRISKTDG--------VCEWKFFSSMPRGSARITVSKDQSSCTT 421
alpha1B SLDDSGSCLSGSQRTLPSASPSPGYLGRGAPPPVELCAFPEWKAPGALLSLPAPEPPGRR 465
beta1 PPPSPGAASDDDDDDVVGATPPAR----------LLEPWAGCNGGAAADSDSSLDEPCRP 469
beta2 ---SGYHVEQEKENKLLCEDLPG------------TEDFVGHQGTVPSDNIDSQGRNCS- 407
beta3 -------------PALFPS------------------------GVPAARSSPAQPRLCQR 399
alpha2A ------------------------------------------------------------
alpha2C ------------------------------------------------------------
alpha2B ------------------------------------------------------------
alpha1A-1 ARVRSKSFLQVCCC-----VGPSTPSLDKNHQVPTIKVHTIS-LSENGEEV---- 466
alpha1A-3 ARGHT----------------PMT------------------------------- 429
alpha1A-4 ARRGMD-----------------CRYFTKNCREHIKHVNFMMPPWRKGSEC---- 455
alpha1A-2 ARTKSRSVTRLECSGMILAHCNLRLPGSRDSPASASQAAGTTGMCHQADATRPS- 475
alpha1B GRHDSGPLFTFKLLTEPESPGTDGGASNGGCEAAADVANGQPGFKSNMPLAPGQF 520
beta1 GFASESKV----------------------------------------------- 477
beta2 --TNDSLL----------------------------------------------- 413
beta3 LDGASWGVS---------------------------------------------- 408
alpha2A -------------------------------------------------------
alpha2C -------------------------------------------------------
alpha2B -------------------------------------------------------
Accession numbers of the adrenergic receptors used in the protein sequence alignment: NP_000671 alpha-1A-adrenergic receptor isoform 1; NP_150646 alpha-1A-adrenergic receptor isoform 2; NP_150645 alpha-1A-adrenergic receptor isoform 3; NP_150647 alpha-1A-adrenergic receptor isoform 4; NP_000670 alpha-1B-adrenergic receptor; NP_000672 alpha-2A-adrenergic receptor; NP_000673 alpha-2B-adrenergic receptor; NP_000674 alpha-2C-adrenergic receptor; NP_000675 beta-1-adrenergic receptor; NP_000015 adrenergic, beta-2-, receptor; NP_000016 adrenergic, beta-3-, receptor
Predicted General Properties of the Alpha 1A-1 Adrenergic (Homo sapiens) - Primary Sequence Analysis (with ProtParam)b
Number of amino acids: 466
Molecular weight: 51486.5
Theoretical pI: 9.26
Total number of negatively charged residues (Asp + Glu): 28
Total number of positively charged residues (Arg + Lys): 48
Atomic composition: Carbon C 2340 Hydrogen H 3698 Nitrogen N 616 Oxygen O 633 Sulfur S 28
Formula: C2340H3698N616O633S28
Total number of atoms: 7315
Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 60610 Abs 0.1% (=1 g/l) 1.177, assuming ALL Cys residues appear as half cystines Ext. coefficient 59360 Abs 0.1% (=1 g/l) 1.153, assuming NO Cys residues appear as half cystines
Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo).
Instability index: The instability index (II) is computed to be 44.95 This classifies the protein as unstable.
Aliphatic index: 101.39
Grand average of hydropathicity (GRAVY): 0.307
Bioinformatics References
a Higgins D., Thompson J., Gibson T. Thompson J. D., Higgins D. G., Gibson T. J.(1994). CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting,position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 22:4673-4680.
b Gasteiger E., Hoogland C., Gattiker A., Duvaud S., Wilkins M.R., Appel R.D., Bairoch A.; Protein Identification and Analysis Tools on the ExPASy Server;
(In) John M. Walker (ed): The Proteomics Protocols Handbook, Humana Press (2005). pp. 571-607 Full text - Copyright Humana Press.
