Sequence Alignment of the 5-HT Receptor (with Clustalw)a
Isoform Sequence Alignment
of the 5-HT Receptor
5HT1A --------------MDVLSPGQGNNTTSPPAPFETGGNTTGISDVTVSY----------- 35
5HT1B MEEPGAQCAPPPPAGSETWVPQANLSSAPSQNCSAKDYIYQDS-ISLPW----------- 48
5HT1D ---------MSPLNQSAEGLPQE--ASNRSLNATETSEAWDPR-TLQAL----------- 37
5HT1E ---------------------------MNITNCT-TEASMAIRPKTITE----------- 21
5HT1F ---------------------------MDFLNSSDQNLTSEELLNRMPS----------- 22
5HT2A MDILCEENTSLSSTTNSLMQLNDDTRLYSNDFNSGEANTSDAFNWTVDSENRTNLSCEGC 60
5HT2B -MALSYRVSELQSTIPEHILQSTFVHVIS-------SNWSGLQTESIPEEMKQIVEEQG- 51
5HT2C MVNLRNAVHSFLVHLIGLLVWQCDISVSP-----VAAIVTDIFN-TSDG-GRFKFPDG-- 51
5HT3A ---MLLWVQQALLALLLPTLLAQGEARRSRNTTRPALLRLSDYLLTNYR----------- 46
5HT3B ---MLSSVMAPLWACIL--VAAGILATDTHHPQDSALYHLSKQLLQKYH----------- 44
5HT3C MEGGWPARQSALLCLTVSLLLQGRGDAFTINCSGFDQHGVDPAVFQAVFD---------- 50
5HT3D MQKHSPG-PPALALLSQSLLTTGNGDTLIINCPGFGQHRVDPAAFQAVFD---------- 49
5HT3E MEGSWFHRKRFSFYLLLGFLLQGRGVTFTINCSGFGQHGADPTALNSVFN---------- 50
5HT4 ------------------------------MDKLDANVSSEEGFGSVEK----------- 19
5HT5 --------MDLPVN-LTSFSLSTPSPLETNHSLGKDDLRPSSPLLSVFG----------- 40
5HT6 -------------------------MVPEPGPTANSTPAWGAGPPSAPGG---------- 25
5HT7 -------MMDVNSSGRPDLYGHLRSFLLPEVGRGLPDLSPDGGADPVAGSWAPHLLSEVT 53
5HT1A ---------------------------QVITSLLLGTLIFCAVLGNACVVAAIALERSL- 67
5HT1B ---------------------------KVLLVMLLALITLATTLSNAFVIATVYRTRKL- 80
5HT1D ---------------------------KISLAVVLSVITLATVLSNAFVLTTILLTRKL- 69
5HT1E ---------------------------KMLICMTLVVITTLTTLLNLAVIMAIGTTKKL- 53
5HT1F ---------------------------KILVSLTLSGLALMTTTINSLVIAAIIVTRKL- 54
5HT2A LSPSCL--------------SLLHLQEKNWSALLTAVVIILTIAGNILVIMAVSLEKKL- 105
5HT2B -------------------------NKLHWAALLILMVIIPTIGGNTLVILAVSLEKKL- 85
5HT2C --------------------------VQNWPALSIVIIIIMTIGGNILVIMAVSMEKKL- 84
5HT3A ----------------------------KGVRPVRDWRKPTTVSIDVIVYAILNVDEKN- 77
5HT3B ----------------------------KEVRPVYNWTKATTVYLDLFVHAILDVDAEN- 75
5HT3C ---------------------------RKAFRPFTNYSIPTRVNISFTLSAILGVDAQL- 82
5HT3D ---------------------------RKAIGPVTNYSVATHVNISFTLSAIWNCYSRI- 81
5HT3E ---------------------------RKPFRPVTNISVPTQVNISFAMSAILDVNEQL- 82
5HT4 ----------------------------VVLLTFLSTVILMAILGNLLVMVAVCWDRQLR 51
5HT5 ----------------------------VLILTLLGFLVAATFAWNLLVLATILRVRTF- 71
5HT6 ---------------------------SGWVAAALCVVIALTAAANSLLIALICTQPAL- 57
5HT7 ASPAPTWDAPPDNASGCGEQINYGRVEKVVIGSILTLITLLTIAGNCLVVISVCFVKKL- 112
. :
5HT1A QNVANYLIGSLAVTDLMVSVLVLPMAALY--QVLN-KWTLGQVTCDLFIALDVLCCTSSI 124
5HT1B HTPANYLIASLAVTDLLVSILVMPISTMY--TVTG-RWTLGQVVCDFWLSSDITCCTASI 137
5HT1D HTPANYLIGSLATTDLLVSILVMPISIAY--TITH-TWNFGQILCDIWLSSDITCCTASI 126
5HT1E HQPANYLICSLAVTDLLVAVLVMPLSIIY--IVMD-RWKLGYFLCEVWLSVDMTCCTCSI 110
5HT1F HHPANYLICSLAVTDFLVAVLVMPFSIVY--IVRE-SWIMGQVVCDIWLSVDITCCTCSI 111
5HT2A QNATNYFLMSLAIADMLLGFLVMPVSMLT--ILYGYRWPLPSKLCAVWIYLDVLFSTASI 163
5HT2B QYATNYFLMSLAVADLLVGLFVMPIALLT--IMFEAMWPLPLVLCPAWLFLDVLFSTASI 143
5HT2C HNATNYFLMSLAIADMLVGLLVMPLSLLA--ILYDYVWPLPRYLCPVWISLDVLFSTASI 142
5HT3A QVLTTYIWYRQYWTDEFLQWNPEDFDNITKLSIPTDSIWVPDILINEFVDVGKSPNIPYV 137
5HT3B QILKTSVWYQEVWNDEFLSWNSSMFDEIREISLPLSAIWAPDIIINEFVDIERYPDLPYV 135
5HT3C QLLTSFLWMDLVWDNPFINWNPKECVGINKLTVLAENLWLPDIFIVESMDVDQTPSGLTA 142
5HT3D HTFN--CHHARPWHNQFVQWNPDECGGIKKSGMATENLWLSDVFIEES--VDQTPAGLMA 137
5HT3E HLLSSFLWLEMVWDNPFISWNPEECEGITKMSMAAKNLWLPDIFIIELMDVDKTPKGLTA 142
5HT4 KIKTNYFIVSLAFADLLVSVLVMPFGAIE---LVQDIWIYGEVFCLVRTSLDVLLTTASI 108
5HT5 HRVPHNLVASMAVSDVLVAALVMPLSLVH--ELSGRRWQLGRRLCQLWIACDVLCCTASI 129
5HT6 RNTSNFFLVSLFTSDLMVGLVVMPPAMLN---ALYGRWVLARGLCLLWTAFDVMCCSASI 114
5HT7 RQPSNYLIVSLALADLSVAVAVMPFVSVT--DLIGGKWIFGHFFCNVFIAMDVMCCTASI 170
: : :
5HT1A LHLCAIALDRYWAIT-DPIDYVNKRTPRRAAALISLTWLIGFLISIPPMLGWRT------ 177
5HT1B LHLCVIALDRYWAIT-DAVEYSAKRTPKRAAVMIALVWVFSISISLPPFF-WRQ------ 189
5HT1D LHLCVIALDRYWAIT-DALEYSKRRTAGHAATMIAIVWAISICISIPPLF-WRQ------ 178
5HT1E LHLCVIALDRYWAIT-NAIEYARKRTAKRAALMILTVWTISIFISMPPLF-WRS------ 162
5HT1F LHLSAIALDRYRAIT-DAVEYARKRTPKHAGIMITIVWIISVFISMPPLF-WRH------ 163
5HT2A MHLCAISLDRYVAIQ-NPIHHSRFNSRTKAFLKIIAVWTISVGISMPIPVFGLQ------ 216
5HT2B MHLCAISVDRYIAIK-KPIQANQYNSRATAFIKITVVWLISIGIAIPVPIKGIE------ 196
5HT2C MHLCAISLDRYVAIR-NPIEHSRFNSRTKAIMKIAIVWAISIGVSVPIPVIGLR------ 195
5HT3A YIRHQGEVQNYKPLQ-VVTACSLDIYNFPFDVQNCSLTFTSWLHTIQDINISLW------ 190
5HT3B YVNSSGTIENYKPIQ-VVSACSLETYAFPFDVQNCSLTFKSILHTVEDVDLAFL------ 188
5HT3C YISSEGRIKYDKPMR-VTSICNLDIFYFPFDQQNCTFTFSSFLYTVDSMLLGMD------ 195
5HT3D SMS-----IVKATSN-TISQCGWS------ASANWTPSISPSMDRAR----AWR------ 175
5HT3E YVSNEGRIRYKKPMK-VDSICNLDIFYFPFDQQNCTLTFSSFLYTVDSMLLDME------ 195
5HT4 FHLCCISLDRYYAICCQPLVYRNKMTPLRIALMLGGCWVIPTFISFLPIMQGWNNIGIID 168
5HT5 WNVTAIALDRYWSIT-RHMEYTLRTRKCVSNVMIALTWALSAVISLAPLLFGWG------ 182
5HT6 LNLCLISLDRYLLIL-SPLRYKLRMTPLRALALVLGAWSLAALASFLPLLLGWH------ 167
5HT7 MTLCVISIDRYLGIT-RPLTYPVRQNGKCMAKMILSVWLLSASITLPP-LFGWA------ 222
5HT1A --PE---DRSDPDACTISKDHG-YTIYSTFGAFYIPLLLMLVLYGRIFRAARFRIRKTVK 231
5HT1B --AK---AEEEVSECVVNTDHILYTVYSTVGAFYFPTLLLIALYGRIYVEARSRILKQTP 244
5HT1D --AK---AQEEMSDCLVNTSQISYTIYSTCGAFYIPSVLLIILYGRIYRAARNRILNP-P 232
5HT1E --HRR--LSPPPSQCTIQHDHVIYTIYSTLGAFYIPLTLILILYYRIYHAAKSLYQKRGS 218
5HT1F --QG----TSRDDECIIKHDHIVSTIYSTFGAFYIPLALILILYYKIYRAAKTLYHKRQA 217
5HT2A --DDSKVFKE-GSCLLADD---NFVLIGSFVSFFIPLTIMVITYFLTIKSLQKEATLCVS 270
5HT2B --TDVDNPNN-ITCVLTKERFGDFMLFGSLAAFFTPLAIMIVTYFLTIHALQKKAYLVKN 253
5HT2C --DEEKVFVNNTTCVLNDP---NFVLIGSFVAFFIPLTIMVITYCLTIYVLRRQALMLLH 250
5HT3A --RLPEKVKSD-RSVFMNQGEWELLGVLPYFREFSMESSNYYAEMKFYVVIRR--RPLFY 245
5HT3B --RSPEDIQHD-KKAFLNDSEWELLSVSSTYSILQS-SAGGFAQIQFNVVMRR--HPLVY 242
5HT3C --KEVWEITDTSRKVIQTQGEWELLGINKATPKMSM-GNNLYDQIMFYVAIRR--RPSLY 250
5HT3D --RMSRSFQIHHRTSFRTRREWVLLGIQKRTIKVTV-ATNQYEQAIFHVAIRRRCRPSPY 232
5HT3E --KEVWEITDASRNILQTHGEWELLGLSKATAKLSR-GGNLYDQIVFYVAIRR--RPSLY 250
5HT4 LIEKRKFNQNSNSTYCVFMVNKPYAITCSVVAFYIPFLLMVLAYYRIYVTAKEHAHQIQM 228
5HT5 --ET---YSEGSEECQVSREPS-YAVFSTVGAFYLPLCVVLFVYWKIYKAAKFRVGSRKT 236
5HT6 ---ELGHARPPVPGQCRLLASLPFVLVASGLTFFLPSGAICFTYCRILLAARKQAVQVAS 224
5HT7 --QN---VND-DKVCLISQDFG-YTIYSTAVAFYIPMSVMLFMYYQIYKAARKSAAKHKF 275
:
5HT1A KVEKTGADTRHGASPAPQPKKSVNGESGSRNWRLGVESKAGGALCANGAVRQGDDGAALE 291
5HT1B NRTGKRLTRAQ----------LITDSPGSTSSVTSINSRVP-------DVPSES-GSPVY 286
5HT1D SLYGKRFTTAH----------LITGSAG--SSLCSLNSSLH-------EGHSHSAGSPLF 273
5HT1E SRHLSNRSTDS----------QNSFAS----CKLTQTFCVS-------DFSTSDPTTEFE 257
5HT1F SR-IAKEEVNG----------QVLLESGEKSTKSVSTSYVL-------EKSLSDPSTDFD 259
5HT2A DLGTRAK--------------LASFSFLP-------------------QSSLSSEKLFQR 297
5HT2B KPPQRLT--------------WLTVSTVFQRDETPCSSPEKVAMLDGSRKDKALPNSGDE 299
5HT2C GHTEEPP--------------GLSLDFLKCCKRNTAE-----------EENSANPNQDQN 285
5HT3A VVS-------------------LLLPSIFLMVMDIVGFYLP--------PNSGERVSFKI 278
5HT3B VVS-------------------LLIPSIFLMLVDLGSFYLP--------PNCRARIVFKT 275
5HT3C IIN-------------------LLVPSSFLVAIDALSFYLP--------AESENRAPFKI 283
5HT3D VVN-------------------FLVPSGILIAIDALSFYLP--------LESGNCAPFKM 265
5HT3E VIN-------------------LLVPSGFLVAIDALSFYLP--------VKSGNRVPFKI 283
5HT4 LQR----------------------------------------------AGASSESRPQS 242
5HT5 NS----------------------VSPIS--EAVEVKDSAK-------QPQMVFTVRHAT 265
5HT6 LTTG-------------------------MASQASETLQVP-----------RTPRPGVE 248
5HT7 PG----------------------FPRVEPDSVIALNGIVK-------LQKEVEECANLS 306
5HT1A VIEVHRVGNSKEHLPLPSEAGPTPCAPASFERKNERNAEAKRKMALARERKTVKTLG--- 348
5HT1B VNQVK--------------------VRVS------DALLEKKKLMAARERKATKTLG--- 317
5HT1D FNHVK--------------------IKLA------DSALERKRISAARERKATKILG--- 304
5HT1E KFHAS--------------------IRIPPFDNDLDHPGERQQISSTRERKAARILG--- 294
5HT1F KIHST--------------------VRSLRSEFKHEKSWRRQKISGTRERKAATTLG--- 296
5HT2A SIHREP----------------------------GSYTGRRTMQSISNEQKACKVLG--- 326
5HT2B TLMRR-----------------------------TSTIGKKSVQTISNEQRASKVLG--- 327
5HT2C ARRRKK----------------------------KERRPRGTMQAINNERKASKVLG--- 314
5HT3A TLLLG----------------------------------YSVFLIIVSDTLPATAIG--- 301
5HT3B SVLVG----------------------------------YTVFRVNMSNQVPRSVGS--- 298
5HT3C TLLLG----------------------------------YNVFLLMMNDLLPASG----- 304
5HT3D TVLLG----------------------------------YSVFLLMMNDLLPATSTSSHA 291
5HT3E TLLLG----------------------------------YNVFLLMMSDLLPTSG----- 304
5HT4 ADQHS-------------------------------------THRMRTETKAAKTLC--- 262
5HT5 VTFQP---------------------------------EGDTWREQK-EQRAALMVG--- 288
5HT6 SADSR----------------------------------RLATKHSRKALKASLTLG--- 271
5HT7 RLLKH---------------------------------ERKNISIFKREQKAATTLG--- 330
.
5HT1A ------------IIMGTFILCWLPFFIVALVLPFCES--SCHMPTLLGAIINWLGY---- 390
5HT1B ------------IILGAFIVCWLPFFIISLVMPICKD--ACWFHLAIFDFFTWLGY---- 359
5HT1D ------------IILGAFIICWLPFFVVSLVLPICRD--SCWIHPALFDFFTWLGY---- 346
5HT1E ------------LILGAFILSWLPFFIKELIVGLS----IYTVSSEVADFLTWLGY---- 334
5HT1F ------------LILGAFVICWLPFFVKELVVNVCD---KCKISEEMSNFLAWLGY---- 337
5HT2A ------------IVFFLFVVMWCPFFITNIMAVICKESCNEDVIGALLNVFVWIGY---- 370
5HT2B ------------IVFFLFLLMWCPFFITNITLVLCDS-CNQTTLQMLLEIFVWIGY---- 370
5HT2C ------------IVFFVFLIMWCPFFITNILSVLCEKSCNQKLMEKLLNVFVWIGY---- 358
5HT3A ----------TPLIG-VYFVVCMALLVISLAETIFIVRLVHKQDLQQPVPAWLRHLVLER 350
5HT3B ----------TPLIG-HFFTICMAFLVLSLAKSIVLVKFLHDEQRGGQEQPFL------- 340
5HT3C ----------TPLIS-VYFALCLSLMVVSLLETVFITYLLHVATTQPPPMPRWLHS---- 349
5HT3D SLVAPLALMQTPLPAGVYFALCLSLMVGSLLETIFITHLLHVATTQPLPLPRWLHS---- 347
5HT3E ----------TPLIG-VYFALCLSLMVGSLLETIFITHLLHVATTQPPPLPRWLHS---- 349
5HT4 ------------IIMGCFCLCWAPFFVTNIVDPFID----YTVPGQVWTAFLWLGY---- 302
5HT5 ------------ILIGVFVLCWIPFFLTELISPLCS----CDIPAIWKSIFLWLGY---- 328
5HT6 ------------ILLGMFFVTWLPFFVANIVQAVCD-----CISPGLFDVLTWLGY---- 310
5HT7 ------------IIVGAFTVCWLPFFLLSTARPFICGTSCSCIPLWVERTFLWLGY---- 374
: : .::: .
5HT1A ------SNSLLNPVIYAYFNKDFQNAFKKIIKCKFCRQ---------------------- 422
5HT1B ------LNSLINPIIYTMSNEDFKQAFHKLIRFKCTS----------------------- 390
5HT1D ------LNSLINPIIYTVFNEEFRQAFQKIVPFRKAS----------------------- 377
5HT1E ------VNSLINPLLYTSFNEDFKLAFKKLIRCREHT----------------------- 365
5HT1F ------LNSLINPLIYTIFNEDFKKAFQKLVRCRC------------------------- 366
5HT2A ------LSSAVNPLVYTLFNKTYRSAFSRYIQCQYKENKKP-LQLILVNTIPALAYKSSQ 423
5HT2B ------VSSGVNPLVYTLFNKTFRDAFGRYITCNYRATKSVKTLRKRSSKIYFRNPMAEN 424
5HT2C ------VCSGINPLVYTLFNKIYRRAFSNYLRCNYKVEKKPPVRQIPRVAATALSGRELN 412
5HT3A IAWLLCLREQSTSQRPPATSQATKTDDCSAMGNHCSHMGGPQDFEKSPRDRCSPPPPPRE 410
5HT3B -----CLRGDTDADRPRVEPRAQR-----AVVTESSLYG----------EHLAQPGTLKE 380
5HT3C ----LLLHCTSPGRCCPTAPQKGNK----GLGLTLTHLPGP----KEPGELAGKKLGPRE 397
5HT3D ----LLLHCTGQGRCCPTAPQKGNK----GPGLTPTHLPGV----KEPEVSAGQMPGPGE 395
5HT3E ----LLLHCNSPGRCCPTAPQKENK----GPGLTPTHLPGV----KEPEVSAGQMPGPAE 397
5HT4 ------INSGLNPFLYAFLNKSFRRAFLIILCCDDERYRRPSILGQTVPCSTTTING--- 353
5HT5 ------SNSFFNPLIYTAFNKNYNSAFKNFFSRQH------------------------- 357
5HT6 ------CNSTMNPIIYPLFMRDFKRALGRFLPCPRCPRERQASLASPSLRTSHSGPRPGL 364
5HT7 ------ANSLINPFIYAFFNRDLRTTYRSLLQCQYRNINRKLSAAGMHEALKLAERPERP 428
. .
5HT1A ------------------------------------------------------------
5HT1B ------------------------------------------------------------
5HT1D ------------------------------------------------------------
5HT1E ------------------------------------------------------------
5HT1F ------------------------------------------------------------
5HT2A LQMGQKK-----------NSKQDAKTTDNDCSMVALGKQHSEEASKDNSDGVNEKVSCV- 471
5HT2B SKFFKKHGIRNGINPAMYQSPMRLRSSTIQSSSIIL--LDTLLLTENEGDKTEEQVSYV- 481
5HT2C VNIYRHT-----------NEPVIEKASDNEPGIEMQ--VENLELPVNPSSVVSERISSV- 458
5HT3A ASLAVCGLLQELSSIRQFLEKRDEIREVARDWLRVGSVLDKLLFHIYLLAVLAYSITLVM 470
5HT3B V-------WSQLQSISNYLQTQDQTDQQEAEWLVLLSRFDRLLFQSYLFMLGIYTITLCS 433
5HT3C TEPD-----GGSGWTKTQ---------LMELWVQFSHAMDTLLFRLYLLFMASSILTVIV 443
5HT3D AELT-----GGSEWTRAQREHEAQKQHSVELWVQFSHAMDALLFRLYLLFMASSIITVIC 450
5HT3E AELT-----GGSEWTRAQREHEAQKQHSVELWLQFSHAMDAMLFRLYLLFMASSIITVIC 452
5HT4 ------------------------STHVLRDAVECGGQWESQCHPPATSPLVAAQPSDT- 388
5HT5 ------------------------------------------------------------
5HT6 SLQQVLPLPLPPDSDSDSDAGSGGSSGLRLTAQLLLPGEATQDPPLPTRAAAAVNFFNID 424
5HT7 EFVLRACTR---------RVLLRPEKRPPVSVWVLQSPDHHNWLADKMLTTVEKKVMIHD 479
5HT1A ----------------
5HT1B ----------------
5HT1D ----------------
5HT1E ----------------
5HT1F ----------------
5HT2A ----------------
5HT2B ----------------
5HT2C ----------------
5HT3A LWSIWQYA-------- 478
5HT3B LWALWGGV-------- 441
5HT3C LWNT------------ 447
5HT3D LWNT------------ 454
5HT3E LWNT------------ 456
5HT4 ----------------
5HT5 ----------------
5HT6 PAEPELRPHPLGIPTN 440
5HT7 ----------------
Accession numbers of the 5HT receptors used in the protein sequence alignment: 5HT1A - P08908; 5HT1B - P28222; 5HT1D - P28221; 5HT1E - P28566; 5HT1F - P30939; 5HT2A - P28223; 5HT2B - P41595; 5HT2C - P28335; 5HT3A - P28335; 5HT3B - O95264; 5HT3C - Q8WXA8; 5HT3D - Q70Z44; 5HT3E - A5X5Y0; 5HT4 - Q13639; 5HT5 - Q13639; 5HT6 - P50406; 5HT7 - P34969
Predicted General Properties of the 5-HT Receptor (5HT1A) - Primary Sequence Analysis (with ProtParam)b
Number of amino acids: 422
Molecular weight: 46106.8
Theoretical pI: 9.13
Total number of negatively charged residues (Asp + Glu): 31
Total number of positively charged residues (Arg + Lys): 44
Atomic composition: Carbon C 2079 Hydrogen H 3311 Nitrogen N 559 Oxygen O 579 Sulfur S 22
Formula: C2079H3311N559O579S22
Total number of atoms: 6550
Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 57255 Abs 0.1% (=1 g/l) 1.242, assuming ALL Cys residues appear as half cystines Ext. coefficient 56380 Abs 0.1% (=1 g/l) 1.223, assuming NO Cys residues appear as half cystines
Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo).
Instability index: The instability index (II) is computed to be 36.52 This classifies the protein as stable.
Aliphatic index: 100.81
Grand average of hydropathicity (GRAVY): 0.195
Bioinformatics References
a Higgins D., Thompson J., Gibson T. Thompson J. D., Higgins D. G., Gibson T. J.(1994). CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting,position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 22:4673-4680.
b Gasteiger E., Hoogland C., Gattiker A., Duvaud S., Wilkins M.R., Appel R.D., Bairoch A.; Protein Identification and Analysis Tools on the ExPASy Server;
(In) John M. Walker (ed): The Proteomics Protocols Handbook, Humana Press (2005). pp. 571-607 Full text - Copyright Humana Press.
